Compound improvement medicament for preventing common crabgrass herb, and preparation method thereof
A technology of medicament and teff, applied in the field of agricultural science, can solve the problems of polluted environment and low activity of biological control bacteria, and achieve the effects of improving yield and quality, improving control efficiency and reducing usage
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Embodiment 1
[0023] Embodiment 1: prepare medicament as follows
[0024] 10 parts Curvularia teff QZ2000, 15 parts Anthracnose Col-68, 20 parts Fusarium tansporum ZC201301, 7.5 parts sucrose, 15 parts soybean oil, 10 parts Tween, 15 parts 30% atrazine wettable powder , 15 parts of 10% refined fluazifop-methyl emulsifiable concentrate, 0.3 parts of sodium methylene dinaphthalene sulfonate, and 200 parts of bentonite.
[0025] The preparation steps are as follows:
[0026] (1) Preparation of the spore suspension of Fusarium pachytansporum: Inoculate the Fusarium pachytansporum ZC201301 strain on a PDA plate medium, culture it in the dark at 26°C for 8 days, and inoculate the meristems on the surface of the medium with sterile water. Wash the spores, centrifuge at 5000rpm for 10min to collect the spores, resuspend them with sterile water, and count to 5×10 8 / mL made conidia suspension;
[0027] (2) Preparation of spore suspension of Curvularia teffensis: inoculate Curvularia teffensis QZ2...
Embodiment 2
[0032] Embodiment 2: prepare the compound medicament of following composition (the concentration of sucrose, soybean powder, Tween is preferred):
[0033] 10 parts of Curvularia teff QZ2000, 15 parts of anthracnose Col-68, 20 parts of Fusarium tansporum ZC201301, 15 parts of 30% atrazine wettable powder, 15 parts of 10% refined fluazifop emulsifiable concentrate, sub 0.3 parts of sodium methyl dinaphthalene sulfonate, 200 parts of bentonite.
[0034] Set sucrose to set gradient: 5, 6, 7, 8, 9, 10 parts by weight;
[0035] Set the gradient of soybean oil: 10, 12, 14, 16, 18, 20 parts by weight;
[0036] Set Tween to set gradient: 5, 6.5, 8, 11.5, 13.5, 15 parts by weight;
[0037] The preparation method of the compound medicament is the same as in Example 1.
[0038] Comparing the effect of promoting the infection rate of crabgrass crabgrass in the compound medicament of comparative example 1 and embodiment 2:
[0039]Dilute the medicament prepared above with water at a rat...
Embodiment 3
[0043] Embodiment 3: prepare the compound medicament of following composition
[0044] 5 parts of Curvularia teff, 10 parts of anthracnose, 15 parts of Fusarium pachytans, 5 parts of sucrose, 10 parts of soybean oil, 5 parts of Tween, 10 parts of atrazine, 10 parts of fluazifop-ethyl, 0.1 part of sodium methylene dinaphthalene sulfonate, 150 parts of bentonite.
[0045] The preparation steps are as follows:
[0046] (1) Preparation of the spore suspension of Fusarium pachytansporum: Inoculate the Fusarium pachytansporum ZC201301 strain on a PDA plate medium, culture it in the dark at 27°C for 8 days, and inoculate the meristems on the surface of the medium with sterile water. Wash the spores, centrifuge at 5000rpm for 10min to collect the spores, resuspend them with sterile water, and count to 5×10 7 / mL made conidia suspension;
[0047] (2) Preparation of spore suspension of Curvularia teffensis: inoculate Curvularia teffensis QZ2000 bacterial strains on PDA plate medium, ...
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