Efficient disease-resistant authenticated inoculum for corn leaf blight and preparation method thereof

Abstract

The invention provides an efficient disease-resistant authenticated inoculum for corn leaf blight and a preparation method thereof. The inoculum comprises the following raw materials in proportion byweight: 100 g of sorghum, 0.20 to 0.25 g of magnesium sulfate, 0.20 to 0.25 g of sodium phosphate and 15 to 30 g of corn leaves. The preparation method comprises the following steps: (1) screening ofcorn leaf blight virulent bacterial strains; (2) storage of corn leaf blight virulent bacterial strains; (3) preparation of secondary cultures; (4) preparation of the inoculum; and (5) cultivation ofthe inoculum. The inoculum has the advantages that the obtained bacterial strains for authenticating corn leaf blight disease resistance are bacterial strains with high pathogenicity, high purity andhigh activity, and guarantees are provided for authenticating corn disease resistance inoculation; the work efficiency is greatly improved compared with a plate medium; the infection of gnats and infectious microbes is avoided, so that the high-quality inoculum finished product is obtained; the cultivation of the inoculum and a cultivation condition thereof are beneficial for generation of corn leaf blight germ conidium, so that the inoculum is high in spore formation speed, large in spore formation quantity and long in spore formation period.

Description

technical field [0001] The invention belongs to the technical field of plant pathogenic fungus research, and in particular relates to an inoculum for identification of high-efficiency disease resistance of maize spot disease and a preparation method thereof. Background technique [0002] Corn (Zea mays L.) is the main grain and feed crop in China, and it is also an important raw material for light industry and pharmaceutical industry. Its planting area and total output are second only to wheat and rice, ranking third. [0003] Southern corn leaf blight (SCLB) is one of the most common maize diseases in the world, and it is an important leaf disease in warm and humid maize production areas in my country. After tasseling, it mainly damages corn leaves, leaf sheaths and bracts. In mild cases, the thousand-grain weight will be reduced, and in severe cases, ears will rot or droop, seeds will turn black, and the germination rate will decrease. Generally, it can reduce corn yield by...

AI Technical Summary

Problems solved by technology

[0005] In the disease resistance identification experiment, usually randomly isolated Pythora maize is used as the test strain, but sometimes the strains used are not strong in pathogenicity, and the effect of disease resistance identification is not ideal; in the process of pathogen isolation, dilute single spore isolation, it is not sure whether the single cell pure culture is obtained

In the preservation of pathogens, PDA medium is often used for cultivation and preservation, but because of the high nutrition of PDA medium, the growth of pathogens is vigorous, and the vitality of the strains is easy to decline after preservation.