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Large-scale preparation method of paecilomyces lilacinus

A technology for Paecilomyces lilacinus and Paecilomyces lilacinus, which is applied in the field of large-scale preparation of Paecilomyces lilacinus, can solve the problems of high environmental requirements, complex production conditions, unfavorable production and the like, and achieves easy thermal insulation, Low equipment dependence, beneficial effects on oxygen supply and exhaust emission

Inactive Publication Date: 2018-09-25
北京四纬生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the preparation method of Paecilomyces lilacinus has high requirements on the environment, complex production conditions and high cost, which is not conducive to large-scale production

Method used

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  • Large-scale preparation method of paecilomyces lilacinus

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preparation example Construction

[0027] A large-scale preparation method of Paecilomyces lilacinus, comprising the steps of:

[0028] Inoculating the Paecilomyces lilacinus strain into the sterilized liquid medium for seed culture to obtain liquid seeds;

[0029] Inoculating the liquid seeds into a solid medium with corn cob powder as the main raw material for solid-state fermentation, the solid medium is sterilized, and the inoculum size is 1%-15%, to obtain a fermentation product;

[0030] The fermented product is made into powder by grinding after drying at low temperature.

[0031] What should be explained here is that the solid culture medium using corncob powder as the main raw material has a high fluffiness, which is conducive to the absorption and maintenance of water, thereby greatly reducing the requirements for the humidity of the culture environment. Corn cob meal is derived from agricultural waste and is inexpensive. In addition, turning waste corn cob powder into treasure has great application...

Embodiment 1

[0049] The invention provides a large-scale preparation method of Paecilomyces lilacinus, comprising the following steps:

[0050] The preserved Paecilomyces lilacinus strain is inoculated in a culture bottle equipped with a PDA slant medium, cultivated at 26°C for 9 days to carry out activation culture, and completes strain activation;

[0051] Inoculate into 1L shake flasks with 400mL of liquid medium, culture at 26°C, 150rpm for 7 days, and the inoculum size is 2%; Inoculate the seed liquid in five 1L shake flasks into a 100L seed fermenter with 60L of liquid medium cultured at 26°C and 150rpm for 6 days; the liquid culture medium is made of the following raw materials in percentage by weight: 1% corn flour, 0.01% urea, 3% glucose, 2% ammonium sulfate, and 2% diammonium hydrogen phosphate , 0.01% yeast powder, and the balance is water.

[0052] What should be explained here is: increasing the sulfur content in the liquid medium helps to increase the sulfur content in the s...

Embodiment 2

[0060] The invention provides a large-scale preparation method of Paecilomyces lilacinus, comprising the following steps:

[0061] The preserved Paecilomyces lilacinus strain is inoculated in a culture bottle equipped with a PDA slant medium, cultivated at 28°C for 6 days for activation and cultivation, and completes the activation of the strain;

[0062] Inoculate into 1L shake flasks with 400mL of liquid medium, culture at 28°C and 150rpm for 5 days, and the inoculum size is 5%; Inoculate the seed liquid in five 1L shake flasks into a 100L seed fermenter with 60L of liquid medium cultured at 28°C and 150rpm for 5 days; the liquid medium is made of the following raw materials in percentage by weight: 0.01% corn flour, 0.01% urea, 0.01% glucose, 0.01% ammonium sulfate, and 0.01% diammonium hydrogen phosphate , 3% yeast powder, and the balance is water. What I want to explain here is: increasing the content of yeast powder helps to make the fermentation effect better.

[0063...

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Abstract

The invention relates to the technical field of biology, and comprises the following steps of inoculating a sterilized fluid nutrient medium with a paecilomyces lilacinus strain to perform seed culture to obtain liquid seed; inoculating a solid nutrient medium, which takes corncob powder as a main raw material with the liquid seed to perform solid state fermentation, wherein the solid nutrient medium is subjected to sterilization processing, and the inoculum size is 1% to 5%, so as to obtain a fermentation product; drying the obtained fermentation product at low temperature and grinding to obtain powder. A large-scale preparation method of paecilomyces lilacinus provided by the invention has the advantages that the cost is low, the environmental requirement is low and the production process is simple.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a large-scale preparation method of Paecilomyces lilacinus. Background technique [0002] The description of the background technology of the present invention belongs to the relevant technology related to the present invention, and is only used to illustrate and facilitate the understanding of the content of the present invention, and should not be understood as the applicant's definite or presumed that the applicant believes that the present invention was filed for the first time. prior art as of the filing date. [0003] Paeci lomyces lilacinus is an important biocontrol pesticide, which plays an important role in human life. [0004] The current preparation method of Paecilomyces lilacinus has high requirements on the environment, complex production conditions and high cost, which is not conducive to large-scale production. Contents of the invention [0005] The purpo...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/79
CPCC12N1/14
Inventor 沈显贵李杰之郭希民沈怡乐
Owner 北京四纬生物科技有限公司
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