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A kind of inoculation method of tobacco brown spot bacteria

A technology of tobacco red spot bacteria and strains, applied in the field of biochemistry, can solve the problems of tobacco difficulty and unfavorable large-scale inoculation

Active Publication Date: 2021-08-24
GUIZHOU TOBACCO SCI RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, it is difficult to inoculate tobacco with Alternaria Alternaria using the above scheme, and it is not conducive to large-scale inoculation

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  • A kind of inoculation method of tobacco brown spot bacteria

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Embodiment Construction

[0023] The principles and features of the present invention are described below in conjunction with the accompanying drawings, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0024] The embodiment is basically as attached figure 1 Shown:

[0025] Tobacco Alternaria inoculation method in the present embodiment, comprises the following steps:

[0026] S1: Obtain the test strain;

[0027] S2: Cultivate the test strain on the culture medium at a preset temperature for a preset time to obtain a conidia petri dish;

[0028] S3: Store the conidia culture dish in an environment at 4°C;

[0029] S4: Obtain last year's sick plant or tobacco leaf of Alternaria spp. and crush it to obtain diseased plant powder;

[0030] S5: The conidia culture dish includes conidia and conidium medium, and the conidia, conidia medium and diseased plant powder are mixed to obtain inoculated spores;

[0031] S6: ...

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Abstract

The invention relates to a method for inoculating tobacco Alternaria Alternaria, in particular to the field of biochemistry. The method comprises the following steps: S1: obtaining the test strain; S2: cultivating the test strain on the culture medium with a preset temperature until a preset time to obtain a conidia culture dish; S3: placing the conidia culture dish Preserved in an environment of 4°C; S4: Obtain the residual strain or tobacco leaf of Alternaria Alternaria from the previous year and pulverize it to obtain powder of the diseased plant; S5: the conidia culture dish includes conidia and conidia medium, mix the Conidia, the conidia medium and the diseased plant powder are obtained to inoculate spores; S6: obtain the plant to be inoculated, and when the lower leaves of the plant to be inoculated begin to mature, sprinkle the inoculated spores on the plant to be inoculated Inoculate the middle and lower mature leaves of the plant. This solution solves the technical problem of how to reduce the difficulty of inoculation of Alternaria spp., and is suitable for inoculation of Alternaria spp.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to a method for inoculating tobacco Alternaria Alternaria. Background technique [0002] Tobacco Alternaria Alternaria is a leaf fungal disease caused by Alternaria. In order to prevent and control tobacco Alternaria Alternaria, artificial inoculation is required. In order to solve this problem, in the prior art, the disease-resistant variety Jingyehuang is planted in the protective row around the identified varieties in the disease nursery field. When the lower leaves begin to mature, the Alternaria Alternaria pathogen isolated and cultivated on the medium is made into 1 % glucose spore suspension (spore concentration is 104-106 / mL), spray inoculation in the middle and lower mature leaves of each tested variety, delayed harvesting or not harvesting the foot leaves. The condition investigation was carried out one day before each harvest, and a total of 3 investigations were carried out....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/00A01G7/06
CPCA01G7/00A01G7/06
Inventor 孔德钧吴春陈尧林英超王志红陈懿
Owner GUIZHOU TOBACCO SCI RES INST
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