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Alternaria alternate inoculation method

A technology of tobacco red spot bacteria and diseased strains, applied in the field of biochemistry, can solve the problems of tobacco being difficult, unfavorable for large-area inoculation, etc., and achieves the effects of high difficulty in solving, favorable for large-area inoculation, and convenient operation.

Active Publication Date: 2019-12-31
GUIZHOU TOBACCO SCI RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, it is difficult to inoculate tobacco with Alternaria Alternaria using the above scheme, and it is not conducive to large-scale inoculation

Method used

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  • Alternaria alternate inoculation method

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Embodiment Construction

[0023] The principles and features of the present invention are described below in conjunction with the accompanying drawings, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0024] The embodiment is basically as attached figure 1 Shown:

[0025] Tobacco Alternaria inoculation method in the present embodiment, comprises the following steps:

[0026] S1: Obtain the strains to be tested;

[0027] S2: Cultivate the test strain on the culture medium at a preset temperature for a preset time to obtain a conidia petri dish;

[0028] S3: Store the conidia culture dish in an environment at 4°C;

[0029] S4: Obtain last year's sick plant or tobacco leaf of Alternaria spp. and crush it to obtain diseased plant powder;

[0030] S5: The conidia culture dish includes conidia and conidium medium, and the conidia, conidia medium and diseased plant powder are mixed to obtain inoculated spores;

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Abstract

The invention relates to an alternaria alternate inoculation method, in particular to the field of biochemistry. The method includes steps: S1, acquiring a strain for testing; S2, culturing the strainfor testing on a culture medium at a preset temperature by preset time to obtain a conidium petri dish which comprises conidia and a meristem culture medium; S3, keeping the conidium petri dish in anenvironment at 4 DEG C; S4, acquiring alternaria alternate infected plants or tobacco leaves obtained last year, and grinding into infected plant powder; S5, mixing the conidia, the meristem culturemedium and the infected plant powder to obtain inoculation spores; S6, acquiring to-be-inoculated plants, and when lower leaves of the to-be-inoculated plants start to mature, spreading the inoculation spores on middle and lower mature leaves of the to-be-inoculated plants. By adoption of the scheme, the technical problem of how to lower alternaria alternate inoculation difficulty is solved, and the method is applicable to alternaria alternate inoculation.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to a method for inoculating tobacco Alternaria Alternaria. Background technique [0002] Tobacco Alternaria Alternaria is a leaf fungal disease caused by Alternaria. In order to prevent and control tobacco Alternaria Alternaria, artificial inoculation is required. In order to solve this problem, in the prior art, the disease-resistant variety Jingyehuang is planted in the protective row around the identified varieties in the disease nursery field. When the lower leaves begin to mature, the Alternaria Alternaria pathogen isolated and cultivated on the medium is made into 1 % glucose spore suspension (spore concentration is 104-106 / mL), spray inoculation in the middle and lower mature leaves of each tested variety, delayed harvesting or not harvesting the foot leaves. The condition investigation was carried out one day before each harvest, and a total of 3 investigations were carried out....

Claims

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Application Information

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IPC IPC(8): A01G7/00A01G7/06
CPCA01G7/00A01G7/06
Inventor 孔德钧吴春陈尧林英超王志红陈懿
Owner GUIZHOU TOBACCO SCI RES INST
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