221 results about "Triterpenoid Compound" patented technology

The invention relates to the technical field of pharmaceutical preparations, in particular to a multiple nano emulsion adjuvant and a preparation method thereof. The multiple nano emulsion adjuvant is prepared from 2.73 to 6.37 g of an oil phase matrix based on 100 g of a raw material system, 0.10 to 0.25 g of a sterol compound; 0.212 to 0.424 g of a nonionic surfactant; 0.00050 to 0.00200 g of a triterpenoid saponin type active adjuvant; 0.010 to 0.020 g of an anionic polymer modifier; 0.005 to 0.015 g of a cationic polymer modifier; 70-90 g of a buffer solution; the total mass of the raw materials is 100 g. The nano emulsion adjuvant prepared by the invention can be stored for 6 months at 2-8 DEG C under a low sterol condition, the particle size change does not exceed + 5%, no crystallization or layering is generated, and the stability of the active adjuvant is remarkably improved.

The invention discloses a gynostemma pentaphyllum transcription factor GpMYC65 and application thereof. The nucleotide sequence of the gynostemma pentaphyllum transcription factor GpMYC65 is as shown in SEQ ID NO. 1. The invention also discloses a preparation method of the gynostemma pentaphyllum transcription factor GpMYC65. The amino acid sequence of the protein encoded by the gynostemma pentaphylla transcription factor GpMYC65 is as shown in SEQ ID NO. 2. The heavy recombinant vector contains a gynostemma pentaphylla transcription factor GpMYC65. The invention relates to application of a Gynostemma pentaphyllum transcription factor GpMYC65 in regulating and controlling the content of dammarane type triterpenoid saponin in Gynostemma pentaphyllum. The application comprises the following steps: step 1, constructing an overexpression vector containing a transcription factor GpMYC65 encoding gene; 2, transforming the overexpression vector into gynostemma pentaphyllum leaves by using an agrobacterium-mediated method to obtain genetic transformation hairy roots, and screening and identifying positive hairy roots of an overexpression transcription factor GpMYC65; and 3, carrying out liquid culture on the screened positive hairy roots to obtain transgenic hairy roots of which the dammarane type triterpenoid saponin content is higher than that of the wild hairy roots. The content of dammarane type triterpenoid saponin can be remarkably increased by overexpressing the transcription factor GpMYC65 in the fiveleaf gynostemma herb hairy roots.

The invention relates to an extraction method, a modification method and application of dendrobium nobile outer vesicles. The method comprises the following steps: placing dendrobium nobile leaves in a phosphate buffer solution, crushing and separating to ensure that plant cells are fully broken and outer vesicles are released; then a differential centrifugation method is combined with sucrose gradient centrifugation, finally, the outer vesicles in a sucrose solution with the mass concentration of 30-45% are selected, and the extracted dendrobium nobile outer vesicles are moderate in mass and high in purity. Then cholesterol structural analogues such as sterol compounds and triterpenoid saponin active molecules are embedded into a phospholipid bilayer of the dendrobium nobile outer vesicles to modify the dendrobium nobile outer vesicles, and the modification strategy can effectively improve the stability of the dendrobium nobile outer vesicles in a complex physiological environment; and the treatment effect can be enhanced through the synergism of active molecules and endogenous components of the vesicles.

A method for preparing nanoparticles of triterpenoid small molecules co-assembled with Ce6 and copper ions wrapped in cancer cell membranes and its application. The present invention prepares a pure natural co-assembled nanotransmission system with new morphological characteristics and multiple biological activities - terpenoid compound and photosensitizer co-assembled nanoparticles. In the composite nanoassembly, triterpenoid compounds and photosensitizer Ce6 are assembled into nanoparticles, providing effective and safe chemotherapy and photodynamic therapy. Subsequently, Cu is complexed on the surface of the nanoparticles. 2+ , when Cu is introduced 2+ When activated, the reduced PDT effect caused by excessive glutathione in the tumor consuming reactive oxygen species can be counteracted. Furthermore, it can enhance CDT therapy through a Fenton-like catalytic reaction with overexpressed H₂O₂ at the tumor site. Finally, these molecules are encapsulated by the tumor cell membrane. By constructing CM@OABACe6 / CuNPs with homologous targeting, a triple synergistic platform for cancer treatment using PDT, chemotherapy, and CDT has been created.

This invention discloses tea saponin E3, its isolation and purification method, and its application as a killer of moon jellyfish polyps, belonging to the field of natural product chemistry. The structure of tea saponin E3 is shown below. Derived from plants, it is a natural triterpenoid saponin compound. Compared with tea saponin, it exhibits stronger killing activity against moon jellyfish polyps. Furthermore, its single component and well-defined structure facilitate standardized application and evaluation of potential ecological risks in the biocontrol of moon jellyfish polyps.

The invention belongs to the technical field of medicines, and particularly relates to a triterpenoid extracted from edible and medicinal fungus ganoderma lucidum, a preparation method of the triterpenoid and application of the triterpenoid in the aspect of resisting Alzheimer's disease. The triterpenoid compound is shown as a structural formula I. The triterpenoid compound provided by the invention has remarkable acetylcholin esterase inhibitory activity, is an ideal candidate compound of an acetylcholin esterase inhibitor which is novel in structure and efficient in activity, and has a good application prospect in the aspect of treatment of Alzheimer's disease. # imgabs0 #

The invention discloses a pharmaceutical composition for treating or improving Parkinson's disease and application of the pharmaceutical composition. The triterpenoid saponin compounds and the nervonic acid in different proportions are combined, and experiments prove that multiple indexes of Parkinson mice can be remarkably improved when the triterpenoid saponin compounds and the nervonic acid are combined in a specific proportion, and the effect of synergistically improving the Parkinson disease or the progress of the Parkinson disease is achieved. Therefore, the pharmaceutical composition disclosed by the invention can be used for more effectively treating or improving the Parkinson's disease, and has a wide application prospect in the field of treatment of the Parkinson's disease.

The invention discloses an engineered glycosyl transferase and application thereof in efficient preparation of triterpenoid saponin. The engineered glycosyl transferase is obtained by mutating serine at the 15th site of UGT74AC1 enzyme into alanine, mutating histidine at the 47th site into aspartic acid, mutating leucine at the 48th site into valine, mutating alanine at the 180th site into serine, mutating histidine at the 180th site into aspartic acid, mutating histidine at the 180th site into aspartic acid, mutating histidine at the 180th site into aspartic acid, mutating histidine at the 180th site into aspartic acid The mutant is obtained by mutating serine at the 332 site into proline, phenylalanine at the 367 site into tryptophan and lysine at the 420 site into arginine. According to the invention, the glycosyl transferase with high catalytic activity is obtained by modifying an enzyme engineering technology, and good application of the glycosyl transferase in synthesis of triterpenoid saponin is realized. The glycosyl transferase disclosed by the invention can also be coupled with sucrose synthase, or coupled with N-acetylhexosamine kinase and N-acetylglucosamine-1-uridine phosphate transferase, and acetylglucosamine is used as a glycosyl donor, so that cascade production of triterpenoid saponin with lower cost and higher efficiency is realized.

The invention discloses arabinose glycosyl transferase PnUGT73B4 and application thereof to preparation of panax japonicus saponin IV, the amino acid sequence of the arabinose glycosyl transferase is shown as SEQ ID NO: 1, under the catalysis of the arabinose glycosyl transferase PnUGT73B4, UDP-arabinose is added to a glucuronic acid group on the C3 site of panax japonicus saponin IVa, and the panax japonicus saponin IV is generated; according to the method, the requirement for raw material plants can be reduced, agricultural land is saved, the product is single, preparation of the panax japonicus saponin IV is facilitated, the biosynthesis route of oleanane type triterpenoid saponin is analyzed, and a breakthrough solution is provided for biosynthesis of the panax japonicus saponin IV.

The present invention discloses a preparation method of Ganoderma lucidum spore oil and Grifola frondosa soft capsules based on a cold preparation method. First, Grifola frondosa extract, selenium-enriched yeast, squalene, and Ganoderma lucidum spore oil are subjected to high-speed shear nano-processing and then set aside; subsequently, gelatin is subjected to low-temperature gelation with glycerol, water, and cocoa shell extract to obtain a gelatin solution, and finally, soft capsules are prepared. While ensuring that gelatin is fully dissolved, the present invention effectively avoids the impact of traditional gelation temperature on gel strength, viscosity, three-dimensional structure, production stability, and other properties and application effects. At the same time, the Ganoderma lucidum spore oil and squalene, Grifola frondosa extract, and selenium-enriched yeast are cleverly combined to significantly increase the content of triterpenoid compounds, a characteristic component in the contents, thereby enhancing the liver's metabolic detoxification ability and the body's anti-inflammatory and immunomodulatory effects.

The invention relates to the technical field of concrete materials, in particular to a formula of plateau high-cold high-speed railway bridge pier column anti-crack concrete which comprises the following components: 280-320 kg / m < 3 > of Portland cement, 80-100 kg / m < 3 > of mineral powder, 60-80 kg / m < 3 > of coal ash, 800-850 kg / m < 3 > of machine-made sand with the fineness of 2.6-3.0, 900-1000 kg / m < 3 > of broken stone, 140-160 kg / m < 3 > of water and 4.0-5.0 kg / m < 3 > of polycarboxylic acid water reducing agent. The total use amount of the retarding components in the water reducing agent is 0.8-1.2 kg / m < 3 >, the air entraining agent comprises triterpenoid saponin and a liquid air entraining agent, the total use amount is 0.02-0.05 kg / m < 3 >, and the use amount of the polypropylene fiber is 0.9-1.2 kg / m < 3 >. Through a low water-binder ratio, a composite gelling system, fiber reinforcement and seasonal regulation and control composite admixture technology, the compressive strength, crack resistance and freeze-thaw resistance are remarkably improved, and the composite admixture is suitable for bridge engineering in plateau alpine regions.

The present invention discloses a fermentation method for Antrodia camphorata mycelium and an auxiliary composition for fermentation of Antrodia camphorata. The method provided by the present invention comprises the following steps: S1. subjecting an Antrodia camphorata strain to liquid fermentation for 60-120 hours; S2. adding precursors to the fermented Antrodia camphorata strain liquid fermentation broth, and continuing the fermentation for more than 120 hours to obtain Antrodia camphorata mycelium; the precursors being coenzyme Q0, p-hydroxybenzoic acid, linalool, and α-terpineol, and the fermentation broth after adding the precursors having a coenzyme Q0 content of 100-500 mg / L, a p-hydroxybenzoic acid content of 20-300 mg / L, a linalool content of 0.1-0.5 mg / L, and an α-terpineol content of 0.3-0.7 mg / L. This method adds precursor substances of specific composition and concentration to the liquid fermentation broth of Antrodia cinnamomea at a certain time and combines them with each other, which is beneficial to the secondary metabolism of Antrodia cinnamomea and promotes the synthesis of triterpenoid compounds, thereby increasing the yield. Compared with the existing technology, the yield of triterpenoid compounds produced by Antrodia cinnamomea mycelium is increased, the culture cycle is shortened, and it is more suitable for large-scale production.

The present invention provides triterpenoid compounds in Ganoderma sinense fermented mycelia. The structural formulas of the triterpenoid compounds are Structural Formulas 1, 3, 4, and 7. The present invention also provides the application of the triterpenoid compounds in Ganoderma sinense fermented mycelia as a drug for preventing or treating inflammation; the triterpenoid compounds in the Ganoderma sinense mycelia are one or more of the compounds with Structural Formulas 1-8. The present invention for the first time conducts an anti-inflammatory activity test on the triterpenoid compounds isolated and purified from Ganoderma sinense mycelia, and finds that the compounds with Structural Formulas 1-8 can all inhibit the release of TNF-α and IL-6, and have significant anti-inflammatory activity.

The present invention discloses the application of Ganoderma lucidum lanostane triterpenoids in the preparation of CYP metabolic enzyme inhibitors, belonging to the technical field of pharmaceuticals. The structural formula of this compound is as follows: #imgabs0# The present invention also provides the application of lanostane triterpenoids in the preparation of CYP metabolic enzyme inhibitors, and evaluates their inhibitory effects on various subtypes of CYP enzymes and the intervention of drug metabolism. The lanostane triterpenoids provided by the present invention can effectively inhibit the activities of various CYP metabolic enzymes, and can be used as combination drugs in inhibiting the hepatic metabolism of clinical drugs, providing a reference for the clinical use of Ganoderma lucidum.

The invention belongs to the field of traditional Chinese medicine chemistry and natural product separation, and particularly relates to extraction and separation of a novel triterpenoid compound 25-Etherxy-dammar-20 (22) Z-ene-3beta, 6alpha, 12beta-triol and application of the novel triterpenoid compound 25-Etherxy-dammar-20 (22) Z-ene-3beta, 6alpha, 12beta-triol in myocardial protection drugs. The compound is named as 25-Etherxy-dammar-20 (22) Z-ene-3beta, 6alpha, 12beta-triol, and the structural formula of the compound is shown in the description. The invention provides an extraction and separation method of the compound. The extraction and separation method mainly comprises the following steps: sequentially carrying out solvent extraction, macroporous adsorption resin enrichment, silica gel column chromatography, ODS medium-pressure column chromatography, high performance liquid chromatography separation and purification and the like on ginseng and other traditional Chinese medicine processed products and hydrolysates. The structure of the compound is determined through comprehensive analysis of a high-resolution mass spectrum (HRMS), one-dimensional nuclear magnetic resonance (1H NMR, 13C NMR) and a two-dimensional nuclear magnetic resonance spectrum (such as HSQC, HMBC, ROESY and the like), and the compound is a newly discovered dammarane type triterpene compound. Experiments show that the compound and the composition thereof have a remarkable protection effect on myocardial cell hypoxia / reoxygenation reperfusion injury, and can be used for preparing drugs for preventing or treating myocardial ischemia reperfusion injury and other related diseases.

The invention relates to the technical field of biology, and discloses high-activity black fungus dry powder as well as a preparation and a preparation method thereof, and the black fungus dry powder is prepared by the following steps: selecting high-quality black fungus in northeast as a raw material, and performing primary washing, fine washing, color protection and draining pretreatment at normal temperature in a dark place; carrying out compound enzymolysis by using cellulase and pectinase under a low-temperature acidic condition; power frequency ultrasonic-assisted extraction is set; drying under a vacuum freezing condition until the moisture is less than or equal to 8%; according to the process disclosed by the invention, the raw materials are selected, and multi-step cooperative treatment of low-temperature enzymolysis wall breaking, ultrasonic enhanced mass transfer, vacuum freeze-drying keep-alive and sterile post-treatment is combined, so that active ingredients of polysaccharide, melanin and triterpenoids are fully reserved, and the process is simple and convenient to operate, low in cost and suitable for large-scale production. Therefore, the extraction efficiency and bioavailability are improved, the hygienic safety and storage stability of a finished product are guaranteed, and a foundation is laid for subsequent development of diversified black fungus preparations.

The invention relates to a triterpenoid saponin compound, a preparation method thereof and application of the triterpenoid saponin compound in preparation of anti-neuroinflammation drugs. The triterpenoid saponin compound has a structure as shown in a formula I. An in-vitro anti-neuroinflammation activity experiment proves that the triterpenoid saponin compound has an obvious inhibition effect on lipopolysaccharide (LPS)-induced BV2 cell inflammation, and can be used as a novel anti-neuroinflammation drug and a neurodegenerative disease treatment drug. Formula I

The invention provides an antrodia camphorata sporocarp extract, which is prepared from the following raw materials in parts by weight: 20 to 24 parts of wild ganoderma lucidum extract, 8 to 12 parts of herba taraxaci and 8 to 12 parts of bolete. The antrodia camphorata sporocarp extract disclosed by the invention contains various physiologically active components such as triterpenoids, polysaccharides, superoxide disproportionated enzymes, adenosine, proteins (containing immune proteins), vitamins, trace elements, nucleic acid, lectin, amino acid, sterols, lignin, blood pressure stabilizing substances and the like; the traditional Chinese medicine composition can relieve various tumors such as hangover, gastrointestinal discomfort, heatstroke, hypertension, virus infection, diabetes mellitus, nephritis, liver cirrhosis and liver cancer, has the effects of resisting oxidation, resisting inflammation, inhibiting hypertension, protecting liver nerves, inhibiting tumor growth and the like, can bidirectionally regulate immunity and protect the liver, can also treat tumors at multiple targets, and has a wide application prospect. The discomfort of a patient in an anti-tumor process is reduced.

The application relates to the technical field of natural medicinal chemistry, and specifically discloses a triterpenoid compound and application, and a preparation method of the compound. The preparation method comprises the following steps: drying and crushing schisandra chinensis roots to obtain dried and crushed schisandra chinensis roots, extracting the dried and crushed schisandra chinensis roots by using 70% ethanol water solution under negative pressure cavitation, combining and concentrating the extract to obtain a crude extract, separating the crude extract by using a D101 macroporous adsorption resin, eluting the crude extract by using water, 30% ethanol, 70% ethanol and 90% ethanol in sequence, collecting a 70% ethanol elution part, performing MCI column chromatography on the 70% ethanol elution part, performing gradient elution on the 70% ethanol elution part by using methanol-water, collecting a depigmentation sample, performing ODS column chromatography on the depigmentation sample, performing gradient elution on the depigmentation sample by using water-methanol, collecting a target component, and purifying the target component by using a preparative high performance liquid chromatograph to obtain the target compound. The application separates a novel triterpenoid compound from schisandra chinensis roots for the first time, expands the development range of medicinal resources of schisandra chinensis plants, simultaneously provides a novel structure mother nucleus and a candidate active molecule for anticancer drug research and development, and enriches the research and development reserves of antitumor natural medicines.

The application provides a microcapsule of triterpenoid compounds of Radix Morindae Officinalis and a preparation method thereof. The preparation method is as follows: taking Radix Morindae Officinalis powder as raw material, first extracting with ethanol under ultrasonic, then extracting with ethyl acetate to obtain triterpenoid compounds of Radix Morindae Officinalis; mixing malt dextrin and gum arabic, dissolving in water to obtain a wall material solution; adding the triterpenoid compounds of Radix Morindae Officinalis into the wall material solution, then adding glycerol monostearate, stirring to obtain an emulsion; spray drying the emulsion to obtain the microcapsule of triterpenoid compounds of Radix Morindae Officinalis. The application also provides application of the microcapsule in preparation of antibacterial drugs or antioxidant active drugs. The extracted triterpenoid compounds of Radix Morindae Officinalis and the microcapsule based on the compounds have good antibacterial and antioxidant activities; through the microencapsulation method, the microcapsule has good water solubility, can avoid being attacked by gastric acid, has the effect of being released fully in the small intestine, and has the advantages of high sustained-release stability and high effective component utilization rate.

The application discloses a norchizhiol triterpenoid compound, a preparation method and application thereof. The norchizhiol triterpenoid compound norchizhiol A can be quickly prepared from Ganoderma lucidum. The test proves that the norchizhiol A can inhibit the accumulation of triglyceride in the differentiation process of 3T3L1 preadipocytes. The norchizhiol A can also effectively reduce the weight increase and liver coefficient of high-fat-diet-induced obese mice, regulate the blood lipid level of the obese mice, and significantly intervene and improve the lipoprotein distribution of the body. The compound can be used for preparing anti-obesity and lipid-lowering medicines.

The present invention belongs to the field of plant genetic engineering technology, and in particular to the application of a Centella asiatica glycosyltransferase gene in catalyzing the glycosylation of asiatic acid and madecassic acid. The nucleotide sequence of the gene is as shown in SEQ ID No.1, and the protein encoded by it can catalyze the C-28 position of asiatic acid and madecassic acid to carry out glucose group modification to produce asiatic acid monoglucoside and madecassic acid monoglucoside, and prefers catalysis madecassic acid. The present invention, by building a metabolic regulatory network, widely and accurately screens the above-mentioned gene, resolves the glycosylation process of catalyzing asiatic acid and madecassic acid, for the in vitro synthesis of Centella asiatica triterpenoid saponins and industrial production process to improve asiatic acid and madecassic acid glycosylation efficiency and asiaticoside and madecassin yield provide important reference basis, and provide important theoretical support for large-scale production of Centella asiatica active saponins.

Sesterterpenoid compounds and an extract L01 of Leucosceptrum canum, and use thereof in treatment of psoriasis are provided, belonging to the technical field of natural medicinal chemistry. The sesterterpenoid compound of the Leucosceptrum canum has a structure shown in any one of Formulas 9 to 12, which shows a highly rare backbone type, exhibits anti-inflammatory and immunosuppressive activities, and can be used in preparation of an anti-inflammatory drug and an immunosuppressive drug. A Leucosceptrum canum extract L01 having compounds 1 to 12 as characteristic ingredients, and use thereof in preparation of an anti-inflammatory drug and an immunosuppressive drug are further provided.

The application provides a varicella-zoster virus vaccine and application thereof, and belongs to the technical field of vaccines.The varicella-zoster virus vaccine comprises liposome nanoparticles, zoster virus glycoprotein E and an adjuvant encapsulated in the liposome nanoparticles.The adjuvant comprises triterpenoid saponins.In the application, the zoster virus glycoprotein E (gE) is wrapped by the liposome nanoparticles, which can effectively promote antigen-presenting cells to phagocytose and efficiently deliver the antigen, and realize sustained stimulation of the vaccine to the body to produce a specific cellular immune response against VZV-gE.The triterpenoid saponins used in the application can effectively realize cross-presentation of the antigen zoster virus glycoprotein E and induce an antigen-specific cellular immune response.In addition, the cholesterol rich in the liposome nanoparticles can effectively neutralize the cytotoxicity of the triterpenoid saponins, ensuring the safety of the vaccine.

The present invention provides a stable emulsion of a triterpenoid compound in a water-in-oil self-emulsifying type and a preparation method thereof. The emulsion uses the triterpenoid compound as an emulsifier. The preparation method includes the following steps: dispersing triterpenoid compound particles in a liquid edible vegetable oil under continuous stirring at a specific temperature to obtain a triterpenoid compound dispersion system; mixing the triterpenoid compound dispersion system obtained in step 1 with an aqueous phase in a certain proportion, and obtaining a stable W / O emulsion after treatment under specific homogenization conditions, and immediately storing it in a sealed manner. The present invention uses the triterpenoid compound as an emulsifier or a gelling agent to stabilize the emulsion or the high internal phase emulsion gel, and the obtained stable emulsion improves the bioavailability of the triterpenoid compound.

The application provides a key enzyme involved in glycosylation modification of triterpene saponins, and an application thereof, and belongs to the technical field of genetic engineering. The glycosyltransferase gene is screened based on the omics research of the base plant of traditional Chinese medicine Duanxueliu, i.e. clinopodium gracile, and a pET28a-MBP-CcGT-g51295 prokaryotic expression vector is constructed to successfully obtain the glycosyltransferase. It is found that the glycosyltransferase can catalyze Saikogenin A and Saikogenin F to generate Prosaikogenin A and Prosaikogenin F respectively by using a glycosyl donor UDP-fucose; can catalyze Saikogenin A to generate clinopodiside I and clinopodiside X by using UDP-glucose; can catalyze Prosaikogenin A to generate Saikosaponin B1, Saikosaponin c1 and Saikosaponin X by using UDP-glucose; and can catalyze Prosaikogenin F to generate Saikosaponin A and Buddlejasaponin IV by using UDP-glucose, which reveals the catalytic function of the glycosyltransferase and provides a gene component for the biological research of triterpene saponins, and solves the problem of biological resources of rare saponins.

The invention discloses a dammarane type triterpenoid compound or a pharmaceutically acceptable salt thereof. The dammarane type triterpenoid compound has a structural formula as shown in a formula I which is described in the specification. The triterpenoids which are extracted and separated from a gynostemma pentaphyllum medicinal material and have a new chemical structure have an obvious effect of promoting ulcer healing, the application range of gynostemma pentaphyllum can be expanded by developing the triterpenoids into medicines for treating diseases and symptoms related to ulcer, and the utilization value of gynostemma pentaphyllum plant resources is improved. # imgabs0 #

The present application provides vaccines comprising an inactivated influenza virus and an adjuvant comprising a triterpenoid saponin, a sterol, and an immunostimulatory oligonucleotide comprising CpG. Also provided are methods of using such vaccines.

The invention discloses biomedical application of 3, 29-dibenzoyl karounitriol, and particularly relates to application of 3, 29-dibenzoyl karounitriol in inhibition of physiological activities of coronavirus and application of 3, 29-dibenzoyl karounitriol in preparation of drugs for preventing and treating coronavirus infectious diseases. The invention proposes that the triterpenoid 3, 29-dibenzoyl karounitriol can inhibit the replication of porcine epidemic diarrhea virus, porcine transmissible gastroenteritis virus and porcine delta coronavirus for the first time; experimental data show that the compound can effectively prevent and treat porcine epidemic diarrhea and has a good application prospect in the domestic pig breeding industry.

To provide wound-healing formulations containing solid birch bark extracts that may be applied to the skin without worsening the patient's condition.SOLUTION: A solid birch bark extract comprises at least about 70 wt.% of betulin, and one or more triterpenes selected from the group consisting of betulinic acid, oleanolic acid, erythrodiol, and lupeol.SELECTED DRAWING: None