92 results about "Triterpenoid Compound" patented technology

The invention relates to a triterpenoid saponin compound, a preparation method thereof and application of the triterpenoid saponin compound in preparation of anti-neuroinflammation drugs. The triterpenoid saponin compound has a structure as shown in a formula I. An in-vitro anti-neuroinflammation activity experiment proves that the triterpenoid saponin compound has an obvious inhibition effect on lipopolysaccharide (LPS)-induced BV2 cell inflammation, and can be used as a novel anti-neuroinflammation drug and a neurodegenerative disease treatment drug. Formula I

The invention provides an antrodia camphorata sporocarp extract, which is prepared from the following raw materials in parts by weight: 20 to 24 parts of wild ganoderma lucidum extract, 8 to 12 parts of herba taraxaci and 8 to 12 parts of bolete. The antrodia camphorata sporocarp extract disclosed by the invention contains various physiologically active components such as triterpenoids, polysaccharides, superoxide disproportionated enzymes, adenosine, proteins (containing immune proteins), vitamins, trace elements, nucleic acid, lectin, amino acid, sterols, lignin, blood pressure stabilizing substances and the like; the traditional Chinese medicine composition can relieve various tumors such as hangover, gastrointestinal discomfort, heatstroke, hypertension, virus infection, diabetes mellitus, nephritis, liver cirrhosis and liver cancer, has the effects of resisting oxidation, resisting inflammation, inhibiting hypertension, protecting liver nerves, inhibiting tumor growth and the like, can bidirectionally regulate immunity and protect the liver, can also treat tumors at multiple targets, and has a wide application prospect. The discomfort of a patient in an anti-tumor process is reduced.

The application relates to the technical field of natural medicinal chemistry, and specifically discloses a triterpenoid compound and application, and a preparation method of the compound. The preparation method comprises the following steps: drying and crushing schisandra chinensis roots to obtain dried and crushed schisandra chinensis roots, extracting the dried and crushed schisandra chinensis roots by using 70% ethanol water solution under negative pressure cavitation, combining and concentrating the extract to obtain a crude extract, separating the crude extract by using a D101 macroporous adsorption resin, eluting the crude extract by using water, 30% ethanol, 70% ethanol and 90% ethanol in sequence, collecting a 70% ethanol elution part, performing MCI column chromatography on the 70% ethanol elution part, performing gradient elution on the 70% ethanol elution part by using methanol-water, collecting a depigmentation sample, performing ODS column chromatography on the depigmentation sample, performing gradient elution on the depigmentation sample by using water-methanol, collecting a target component, and purifying the target component by using a preparative high performance liquid chromatograph to obtain the target compound. The application separates a novel triterpenoid compound from schisandra chinensis roots for the first time, expands the development range of medicinal resources of schisandra chinensis plants, simultaneously provides a novel structure mother nucleus and a candidate active molecule for anticancer drug research and development, and enriches the research and development reserves of antitumor natural medicines.

The application discloses a norchizhiol triterpenoid compound, a preparation method and application thereof. The norchizhiol triterpenoid compound norchizhiol A can be quickly prepared from Ganoderma lucidum. The test proves that the norchizhiol A can inhibit the accumulation of triglyceride in the differentiation process of 3T3L1 preadipocytes. The norchizhiol A can also effectively reduce the weight increase and liver coefficient of high-fat-diet-induced obese mice, regulate the blood lipid level of the obese mice, and significantly intervene and improve the lipoprotein distribution of the body. The compound can be used for preparing anti-obesity and lipid-lowering medicines.

Sesterterpenoid compounds and an extract L01 of Leucosceptrum canum, and use thereof in treatment of psoriasis are provided, belonging to the technical field of natural medicinal chemistry. The sesterterpenoid compound of the Leucosceptrum canum has a structure shown in any one of Formulas 9 to 12, which shows a highly rare backbone type, exhibits anti-inflammatory and immunosuppressive activities, and can be used in preparation of an anti-inflammatory drug and an immunosuppressive drug. A Leucosceptrum canum extract L01 having compounds 1 to 12 as characteristic ingredients, and use thereof in preparation of an anti-inflammatory drug and an immunosuppressive drug are further provided.

The application provides a varicella-zoster virus vaccine and application thereof, and belongs to the technical field of vaccines.The varicella-zoster virus vaccine comprises liposome nanoparticles, zoster virus glycoprotein E and an adjuvant encapsulated in the liposome nanoparticles.The adjuvant comprises triterpenoid saponins.In the application, the zoster virus glycoprotein E (gE) is wrapped by the liposome nanoparticles, which can effectively promote antigen-presenting cells to phagocytose and efficiently deliver the antigen, and realize sustained stimulation of the vaccine to the body to produce a specific cellular immune response against VZV-gE.The triterpenoid saponins used in the application can effectively realize cross-presentation of the antigen zoster virus glycoprotein E and induce an antigen-specific cellular immune response.In addition, the cholesterol rich in the liposome nanoparticles can effectively neutralize the cytotoxicity of the triterpenoid saponins, ensuring the safety of the vaccine.

The application provides a key enzyme involved in glycosylation modification of triterpene saponins, and an application thereof, and belongs to the technical field of genetic engineering. The glycosyltransferase gene is screened based on the omics research of the base plant of traditional Chinese medicine Duanxueliu, i.e. clinopodium gracile, and a pET28a-MBP-CcGT-g51295 prokaryotic expression vector is constructed to successfully obtain the glycosyltransferase. It is found that the glycosyltransferase can catalyze Saikogenin A and Saikogenin F to generate Prosaikogenin A and Prosaikogenin F respectively by using a glycosyl donor UDP-fucose; can catalyze Saikogenin A to generate clinopodiside I and clinopodiside X by using UDP-glucose; can catalyze Prosaikogenin A to generate Saikosaponin B1, Saikosaponin c1 and Saikosaponin X by using UDP-glucose; and can catalyze Prosaikogenin F to generate Saikosaponin A and Buddlejasaponin IV by using UDP-glucose, which reveals the catalytic function of the glycosyltransferase and provides a gene component for the biological research of triterpene saponins, and solves the problem of biological resources of rare saponins.

The present application provides vaccines comprising an inactivated influenza virus and an adjuvant comprising a triterpenoid saponin, a sterol, and an immunostimulatory oligonucleotide comprising CpG. Also provided are methods of using such vaccines.

The application discloses a triterpenoid compound, and the structure is (3β, 6α, 10α, 12α, 16β, 24E)-4-methylol-6, 12-bis(acetyloxy)-3, 10, 16-trihydroxy-9, 19-cyclo-9, 10-secolanosta-9(11), 24-dien-26-yl β-D-glucopyranoside. The application obtains a new monomer compound with anticancer activity by separating and screening monomer components in leaf of Astragalus membranaceus Bge. var. mongholicus (Regel) H. The application provides a theoretical basis for further in-depth research on components and medicinal activities of the leaf of Astragalus membranaceus Bge. var. mongholicus (Regel) H, and also expands natural plant resources of anticancer drugs.

Provided are a Gynostemma pentaphyllum-derived triterpenoid saponin compound and a use thereof in the preparation of anti-fatigue products. The provided triterpenoid saponin compound is selected from one of compounds 1-29. Also provided are a use of a triterpenoid saponin compound as represented by general formula I in the preparation of anti-fatigue products. Experiments show that the Gynostemma pentaphyllum-derived triterpenoid saponin compound has significant anti-fatigue activity and can be used for preparing anti-fatigue products.

The invention belongs to the technical field of health-care products, and discloses an oligosaccharide selenium ganoderma lucidum capsule for enhancing immunity and inhibiting tumors and a preparation method, and the content of the capsule is prepared from the following raw materials in parts by mass: 150-160 parts of collagen, 80-85 parts of chitosan oligosaccharide, 110-130 parts of ganoderma lucidum extract, 45-55 parts of selenium-enriched yeast, 50-80 parts of maltodextrin and 7-10 parts of magnesium stearate. The skin barrier can be improved, intercellular communication can be promoted and local immune response can be stimulated through low-molecular peptide of collagen, intestinal flora balance can be adjusted through chitosan oligosaccharide, probiotic growth can be promoted, mucous membrane immunity can be further improved, and finally immune cells can be effectively activated through polysaccharide and triterpenoids in the ganoderma lucidum extract, so that the immunity of a human body is improved. The activity of macrophages and natural killer cells is enhanced, and the multi-target and multi-path immune regulation effect can effectively enhance the defense of the body to pathogenic microorganisms.

This invention discloses an antitumor natural drug composition based on Norway maple and its preparation method, relating to the field of biomedical technology. The compound is selected from at least one of the following structures: 3-O-[β-D-glucopyranosyl-(1→4)-[β-D-galactopyranosyl-(1→2)]]-β-D-glucopyranoside-21-O-acetyl-22-O-angeloyl yrutile alcohol, with the molecular formula C2. 55 H 86 O 23 This invention isolates a class of triterpenoid saponins with a rutinol skeleton from the bark of Norwegian maple, enriching the chemical composition library of maple species. The compounds contain unique oligosaccharide chains and diacyl substitution patterns at C-21 and C-22. One compound has a rare branched acyl group (3-hydroxy-4-methylhexanoyl) at C-21, while the other compound contains an arabinofuranose unit in its C-3 trisaccharide chain. These structural features are rare in natural products and demonstrate excellent structural novelty.

To provide a composition for promoting tight junction formation that promotes formation of tight junctions between epithelial tissue cells.SOLUTION: A composition for promoting tight junction formation for promoting formation of tight junctions between epithelial tissue cells, contains triterpenes and skim milk and / or wheat gluten, thereby solving the problem to be solved.SELECTED DRAWING: None

The invention relates to the technical field of plant extraction, in particular to a method for extracting poria cocos triterpenoids based on a supramolecular solvent and application of the poria cocos triterpenoids. The method comprises the following steps: extracting poria cocos powder by adopting a supramolecular solvent, carrying out solid-liquid separation, and collecting to obtain a liquid containing triterpenoids; the supramolecular solvent consists of a hydrogen bond acceptor, a hydrogen bond donor and water; the hydrogen bond acceptor comprises at least one of betaine and menthol, and the hydrogen bond donor comprises at least one of 1, 2-butanediol, thymol, aminobutyric acid, lactic acid and camphor. The method has the advantages of being high in extraction rate, more environmentally friendly and higher in whitening and anti-inflammatory activity.

The invention provides a method for accurately evaluating ganoderma lucidum spore powder triterpenes. The method comprises the following steps: (1) pretreating ganoderma lucidum spore powder; (2) an ultra-high performance liquid chromatography detection step; (3) obtaining mass spectrum information of the compound; (4) establishing an ultra-high performance liquid chromatography-triple quadrupole mass spectrometry combined analysis method; and (5) a data analysis step. According to the method for accurately evaluating the content of the triterpenes in the ganoderma lucidum spore powder, provided by the invention, accurate analysis of the triterpenes in the ganoderma lucidum spore powder is realized, and a reliable detection technology is provided for evaluation of ganoderma lucidum spore powder raw materials and development of related products.

The invention belongs to the technical field of food processing, and discloses a technology for producing loquat pollen and a solid beverage by adopting a nanotechnology. The process comprises the steps of low-temperature non-thermal synergistic passivation, nano-scale cell wall breaking, active ingredient nano-packaging, flavor directional regulation and control, low-temperature vacuum drying and the like, and through the synergistic effect of a pulsed electric field-ultrasonic composite passivation, chitosan-sodium alginate nano-embedding and a flavor modifier, the flavor is improved; the triterpenoid saponin and flavone are efficiently reserved, the peculiar smell is obviously reduced, and the instant solubility is improved. The biological accessibility of the obtained product is improved by 3.2 times, and the product is stable in color and has a high-value application prospect.

The invention discloses an antiproliferative synergistic antifungal drug composition based on a notopterygium incisum source, and relates to the technical field of drug preparation, the antiproliferative synergistic antifungal drug composition comprises a first active component and a second active component, the first active component is an anti-proliferative active component and is selected from at least one of the following two compounds: class A: giant column alkane sesquiterpenoids; the class B is a cycloartane type triterpenoid compound; the second active component is a synergistic antifungal active component and is composed of an active component A and an active component B; the pharmaceutical composition takes a specific active component from notopterygium incisum as a core, and can accurately induce apoptosis of target cells, efficiently inhibit proliferation of the target cells, remarkably enhance the antibacterial activity of the antifungal drug and greatly reduce the clinical dosage and toxic risk of the antifungal drug; the natural active components are excellent in biocompatibility and high in medication safety, and can synchronously realize dual effects of disease treatment and infection protection.

The invention belongs to the technical field of traditional Chinese medicine pharmacy, and particularly relates to a rhizoma cimicifugae extract grading treatment device. The invention discloses a rhizoma cimicifugae extract grading treatment device, and aims to solve the problems that in the prior art, a rhizoma cimicifugae extract is easy to agglomerate, thermosensitive components are easy to degrade, the grading precision is greatly influenced by flow field fluctuation, and the continuous production stability is poor. The device comprises a multi-dimensional pre-dispersion unit, a low-temperature controlled grading cavity, a dynamic balance centripetal moment adjusting mechanism, a self-adaptive anti-adhesion lining system and a closed-loop circulation flow control system. Through the synergistic effects of high-frequency sound field pre-dispersion, low-temperature jacket temperature control, centrifugation-air resistance dynamic balance separation, micro-vibration adhesion prevention and the like, efficient protection and accurate classification of triterpenoid saponin and phenolic acid components are achieved. The grading precision, the drug effect retention rate and the continuous operation stability can be remarkably improved, and the method is suitable for precise grading of the heat-sensitive traditional Chinese medicine extract.

This invention belongs to the field of pharmaceutical technology and discloses a spirocyclic triterpenoid compound, its preparation method, and its application in the preparation of antidiabetic drugs. This invention uses the whole herb of *Euphorbia humifusa* as the isolation target and employs an efficient and systematic separation and preparation method to enrich terpenoid compounds. A spirocyclic triterpenoid compound was obtained through silica gel, ODS column chromatography, and HPLC. In vitro inhibition of α-glucosidase experiments confirmed that compound I has significant hypoglycemic activity and can be used as an active ingredient in antidiabetic drugs, with broad applications.

The application discloses application of a triperpenoid compound of Ailanthus-type or a glycoside thereof in preparation of a liver-protecting medicine. The application also performs glycosylation modification on the triperpenoid compound of Ailanthus-type to obtain a liver-protecting active triperpenoid glycoside of Ailanthus-type. The compound has potential application value in preparation of a medicine for preventing or treating liver diseases.

This invention belongs to the field of traditional Chinese medicine chemistry and natural product separation technology, specifically relating to a novel triterpenoid compound and its epimerical form from *Eleutherococcus senticosus*, along with its extraction and separation methods and applications. The new compound is named 22α-Hydroxy-19-epi-chiisanogenin, and its epimerical form is named 22α-Hydroxychiisanogenin. The extraction and separation method provided by this invention employs acid hydrolysis / ethanol reflux extraction, macroporous resin column adsorption, silica gel column chromatography, ODS column chromatography, and high-performance liquid chromatography for separation, purification, and preparation. The compound was successfully extracted and separated. This method involves only five steps, is simple and rapid, and mainly uses conventional reagents such as ethanol, dichloromethane, and methanol. The process is simple and environmentally friendly, and the purity of the compound obtained by this method is high, exceeding 90%. Furthermore, studies have shown that this compound has anti-inflammatory effects. Therefore, the compound of this invention can be used as a lead compound for the synthesis of other compounds, as well as a raw material for new drug development and pharmacological activity research, and can also be used to prepare anti-inflammatory drugs.

The invention discloses a class of euphorbia kanane type triterpenoids as well as a preparation method and application thereof. The euphorbia kanane type triterpenoids are extracted and separated from Chinese quassia tree, four of the euphorbia kanane type triterpenoids are new compounds, and the rest of the euphorbia kanane type triterpenoids are known compounds. The applicant finds that most of the compounds show remarkable anti-neuroinflammation activity through activity screening. In an MPTP-induced PD mouse in-vivo model, part of the compounds can significantly improve the impaired movement behavior of PD mice; in addition, MPTP-induced brain tissue inflammatory response can be effectively inhibited, and a protection effect on dopaminergic neurons is achieved.

The invention provides a triterpenoid compound as well as an extraction and separation method and application thereof, and belongs to the technical field of traditional Chinese medicine separation. The preparation method comprises the following steps: by taking mairei as a raw material, extracting with ethanol to obtain a primary extract, dispersing with pure water, and extracting with petroleum ether to obtain a petroleum ether part extract; and then sequentially carrying out silica gel column chromatography, reversed-phase column separation, gel column chromatography and high performance liquid chromatography separation on the petroleum ether part extract, and extracting and separating the multiflorane type triterpenoid compounds as shown in a formula (I) and a formula (II) for the first time. Pharmacological studies show that the triterpenoid provided by the invention has strong inhibitory activity on a plurality of tumor cells, and the minimum IC50 is 0.47 M + / -0.09 M.

The application discloses a composition for inhibiting degradation and odor of ergothioneine, which is compounded by ergothioneine, liquorice extract and cyclodextrin, and the mass ratio of the ergothioneine, the liquorice extract and the cyclodextrin is 1:(0.5-20):(5-200). The composition can improve the stability of the ergothioneine under high-temperature light, light and normal-temperature conditions in a solution and skin care products, inhibit degradation of the ergothioneine, and obviously improve product odor; in addition, the liquorice extract is a plant-derived extract, is widely applied and has good safety, and triterpenoids such as glycyrrhizic acid contained in the liquorice extract can synergistically improve the antioxidant effect of the ergothioneine, so that the composition can be well applied in the field of cosmetics. The application further discloses an application of the composition for inhibiting degradation and odor of ergothioneine.

A method of preparing a triterpenoid compound of formula (I):including a step of converting a compound of formula (II) into the compound of formula (I),wherein R1 and R2 independently represent hydrogen or a protecting group selected from the group consisting of C1-C8 alkyl, allyl, C2-C8 alkenyl, C2-C8 alkynyl, (C6-C12)aryl(C1-C8)alkyl, tri(C1-C8)alkylsilyl, di(C1-C8)alkyl(C6-C12)arylsilyl, di(C6-C12)aryl(C1-C8)alkylsilyl ,tri(C6-C12)arylsilyl, —C(O)R7, and —C(O)OR8, and each of which is substituted with from 0 to 4 substituents independently selected from the group consisting of hydroxy, cyano, halo, halo(C1-C6)alkyl, halo(C1-C6)alkyloxy, (C1-C6)alkylthio, C1-C6 alkyl, C2-C6 alkenyl, C2-C6 alkynyl, C3-C7 cycloalkyl and C1-C6 alkoxy, wherein R7 and R8 are independently C1-C8 alkyl or C6-C12 aryl.

The present invention aims to provide a novel triterpenoid saponin vaccine adjuvant. By means of molecular design, synthesis, activity evaluation, and other means, the adjuvant significantly improves immunostimulatory activity thereof while reducing the potential toxicity thereof and significantly enhancing the stability of a product. Specifically, the adjuvant of the present invention can induce a Th1 / Th2 mixed immune response, effectively improve the ability of an antigen presenting cell (such as a dendritic cell) to uptake, treat, and present an antigen (including but not limited to a microbial pathogen, a cancer cell, etc.), thereby enhancing the cellular immune efficacy and exhibiting great potential for preventing and treating a disease caused by a related pathogen.

The invention belongs to the technical field of natural medicines, and particularly relates to a norkanane type triterpenoid compound as well as an extraction method and application thereof. The structure of the norkanane type triterpenoid is shown as a formula I. The norkanane type triterpenoid is extracted through the following steps: S1, mixing dried and crushed cortex ailanthi with a 95% ethanol solution, performing heating reflux extraction to obtain an extracting solution, and concentrating the extracting solution to obtain an extract; s2, dispersing the extract obtained in the step S1 into water, sequentially extracting with petroleum ether and dichloromethane, collecting dichloromethane extract liquor, and concentrating to obtain a dichloromethane extract; and S3, separating and purifying the dichloromethane extract obtained in the step S2 to obtain a target compound. Experiments prove that the compound disclosed by the invention has good anti-tumor activity and can be applied to preparation of anti-tumor drugs. The extraction method disclosed by the invention is simple, convenient and rapid to operate and has the potential of industrial production.

The application discloses a triterpene glycoside saponin compound or a pharmaceutically acceptable salt, ester or solvate thereof, which is shown as formula I. The application further discloses a preparation method and application of the triterpene glycoside saponin compound shown as formula I.

This invention relates to the field of plant genetic engineering technology, and discloses an AtaSQS gene for the biosynthesis of triterpenoids in Aster tataricus and its applications. The AtaSQS gene includes a complete coding region CDS sequence and a promoter sequence, derived from *Aster tataricus* var. *ji*. The CDS sequence is shown in SEQ ID NO.1. The promoter sequence, 1289 bp in length, is located upstream of the CDS sequence and is used to regulate the spatiotemporally specific expression of the AtaSQS gene. This invention cloned the key gene AtaSQS for the biosynthesis of triterpenoids and its promoter sequence from *Aster tataricus* var. *ji*. It clarified its positive regulatory function on the synthesis of total triterpenoids and asterones, and provided related recombinant expression vectors, host bacteria, and breeding methods. This fills the technical gap in the regulatory mechanism of triterpenoid synthesis in Aster tataricus, and can significantly accelerate the breeding process of Aster tataricus varieties with high triterpenoid content, improve their medicinal quality and economic value, and has important application prospects in the fields of plant genetic engineering and the quality improvement of traditional Chinese medicinal materials.

The invention provides a chlorhematin-containing plant growth regulator as well as a preparation method and application thereof, and relates to the technical field of plant growth regulators. The plant growth regulator containing chlorhematin is prepared from the following raw materials in percentage by mass: 0.2 to 0.5 percent of microencapsulated chlorhematin, 3 to 6 percent of triterpenoid saponin, 1 to 2 percent of oleanolic acid-beta-cyclodextrin inclusion compound, 3 to 6 percent of tea saponin, 2 to 4 percent of sodium lignin sulfonate, 0.2 to 0.5 percent of polyether modified organic silicon defoaming agent, 1 to 3 percent of sodium polyacrylate dispersing agent, 0.5 to 1.5 percent of polyvinyl alcohol and the balance of water. 0.5-1.5% of sodium carboxymethyl cellulose and the balance of pre-gelatinized starch; the microencapsulated hemin wall material consists of sodium alginate, chitosan and gelatin in a mass ratio of 3: (2-3): 1; the triterpenoid saponin is gynostemma pentaphyllum saponin and / or ginsenoside.