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A kind of bacillus amyloliquefaciens preparation and application thereof

A technology for dissolving amyloid spores and bacilli, applied in the application, fungicides, biocides and other directions, can solve the problems of ineffective corn yield increase, easy generation of drug resistance by bacteria, unsafe human and livestock, etc. The effect of lengthening and reducing the protruding tip rate

Active Publication Date: 2017-10-27
刘洪亮
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems that the existing chemical agents in the prevention and treatment of large spot of corn pollute the environment, are unsafe for humans and animals, the bacteria are prone to drug resistance, and the effect on increasing corn production is not obvious, and a kind of Bacillus amyloliquefaciens is provided Preparations and their applications

Method used

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  • A kind of bacillus amyloliquefaciens preparation and application thereof
  • A kind of bacillus amyloliquefaciens preparation and application thereof
  • A kind of bacillus amyloliquefaciens preparation and application thereof

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specific Embodiment approach 1

[0008] Specific embodiment one: a kind of Bacillus amyloliquefaciens preparation of this embodiment comprises 0.01~0.02% zinc sulfate by mass fraction, 0.01~0.02% boric acid, 0.1~0.2% Tween 20 and the Bacillus amyloliquefaciens (Bacillus amyloliquefaciens ) KB1-01 fermented liquid, wherein the component content per 1 liter of the fermentation medium for preparing Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) KB1-01 fermented liquid is: glucose 0.1%, soybean powder 0.4%, peptone 0.1%, yeast extract 0.05 %, 0.12% potassium dihydrogen phosphate, 0.024% magnesium sulfate and the rest of water; the fermentation conditions are: temperature 32°C, pH 7.0-7.2, rotation speed 170r / min, incubation time 48h, inoculum size 2%.

[0009] The invention uses Bacillus amyloliquefaciens KB1-01 to prepare a high-efficiency, broad-spectrum, non-toxic and non-polluting ecological biocontrol microbial agent, which is environmentally friendly and does not cause pathogenic bacteria to develop...

specific Embodiment approach 2

[0010] Specific embodiment two: what this embodiment is different from specific embodiment one is that the Bacillus amyloliquefaciens preparation comprises 0.02% zinc sulfate by mass fraction, 0.02% boric acid, 0.1% Tween 20 and 99.86% Bacillus amyloliquefaciens (Bacillus amyloliquefaciens ) KB1-01 fermentation broth, the others are the same as in Embodiment 1.

specific Embodiment approach 3

[0011] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the Bacillus amyloliquefaciens KB1-01 is preserved in the General Microorganism Center of China Committee for the Preservation of Microorganisms, and the preservation address is Beijing No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, the city, the preservation date is September 12, 2014, and the preservation number is CGMCC No.9649. Others are the same as the specific implementation mode 1 or 2.

[0012] The screening method of Bacillus amyloliquefaciens (Bacillusa myloliquefaciens) KB1-01 in this embodiment is as follows: collect corn rhizosphere soil samples from seven branches of Heilongjiang Agricultural Reclamation Harbin, Hongxinglong, Mudanjiang, Jiusan, Suihua, Bei'an, and Baoquanling 15 servings.

[0013] The collected soil samples were air-dried naturally. Weigh 10g of each soil sample, put it into a triangular flask filled with 90ml of sterile water and glass bea...

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Abstract

A preparation of bacillus amyloliquefaciens and its application. The invention relates to a preparation of bacillus amyloliquefaciens and its application. The purpose of the present invention is to solve the problems that the existing chemical agents in the prevention and treatment of maize leaf blotch pollute the environment, are unsafe for humans and animals, the pathogens are prone to drug resistance, and the effect of increasing corn yield is not obvious. The invention comprises 0.01-0.02% zinc sulfate, 0.01-0.02% boric acid, 0.1-0.2% Tween 20 and the balance of Bacillus amyloliquefaciens KB1-01 according to the mass fraction. The application of the present invention refers to the application of increasing the yield of corn while inhibiting the maize leaf blight. The control effect of the present invention on maize leaf spot is 80.8%, and the yield increase rate of maize is 18.26%. The invention is applied in the field of agriculture.

Description

technical field [0001] The invention relates to a bacillus amyloliquefaciens preparation and application thereof. Background technique [0002] Corn spot disease is also known as streak disease, coal spot disease, dead leaf disease, leaf spot disease and so on. Mainly damage the leaves, leaf sheaths and bracts of corn. Water-soaked blue-gray spots first appear on the leaves, and then expand along the veins to both ends, forming large spots with dark brown edges, light brown or blue-gray centers. Late lesions often split longitudinally. In severe cases, the lesions merge and the leaves turn yellow and die. When wet, there are a large number of gray-black mold layers on the lesions. The lower leaves are affected first. On single-gene disease-resistant varieties, it shows as chlorotic lesions, small lesions, parallel to the veins, yellow-green or light brown in color, dark brown around, and some appear as necrotic spots. [0003] The control methods of maize leaf spot mai...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/02A01P3/00A01P21/00A01N63/00A01N59/14
Inventor 刘洪亮王慧于琳
Owner 刘洪亮
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