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Bacillus amyloliquefaciens and application thereof

A technology of amylolytic spores and bacilli, which is applied in the direction of bacteria, fungicides, and chemicals for biological control, etc., can solve the problems of ineffective increase in corn production, easy resistance of pathogens, and unsafe conditions for humans and animals, and achieve multiple preventions. The occurrence of various diseases, the control effect is good, and the effect of reducing the rate of protrusion

Inactive Publication Date: 2015-12-23
刘洪亮
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of environmental pollution caused by chemical agents in the prevention and treatment of corn leaf spot, unsafe for humans and animals, easy resistance of pathogens, and ineffective effect on increasing corn production, and provides a strain of Bacillus amyloliquefaciens and its application

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  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof

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specific Embodiment approach 1

[0009] Specific embodiment 1: A strain of Bacillus amyloliquefaciens KB1-01 in this embodiment is preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, and the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing. The date is September 12, 2014, and the deposit number is CGMCCNo.9649.

[0010] In this embodiment, Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) KB1-01 was screened out from corn rhizosphere soil samples collected from the seven sub-bureaus of Heilongjiang Agricultural Reclamation Harbin, Hongxinglong, Mudanjiang, Jiusan, Suihua, Bei'an, and Baoquanling. Milky white and opaque, wrinkled and protruding in texture, the shape of the colony is bead-shaped, the center of the colony is raised, and the edges are neat. The DNA of Bacillusamyloliquefaciens KB1-01 was extracted by CTAB method. The 16SrRNA gene was amplified by PCR, and the general primers of the 16SrRNA gene we...

specific Embodiment approach 2

[0012] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the component content of the bacterium per 1 liter of fermentation medium is: 0.1% glucose, 0.4% soybean powder, 0.1% peptone, 0.05% yeast extract, phosphoric acid Potassium dihydrogen 0.12%, magnesium sulfate 0.024% and the rest of water; the fermentation conditions are: temperature 32°C, pH 7.0-7.2, rotation speed 170r / min, culture time 48h, inoculum size 2%. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0013] Embodiment 3: The application of a strain of Bacillus amyloliquefaciens in this embodiment refers to the application of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) KB1-01 to increase the yield of corn while preventing and controlling Bacteria maize.

[0014] In this embodiment, Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) KB1-01 is friendly to the environment and will not cause pathogenic bacteria to develop drug resistance. In addition, the (Bacillus amyloliquefaciens) KB1-01 flora can rapidly reproduce and occupy sites, secrete a variety of antibiotic substances, and prevent A variety of diseases occur; it can be prepared into a high-efficiency, broad-spectrum, non-toxic, and non-polluting ecological biocontrol microbial agent. The application effect of the agent on corn shows that through the field drug efficacy test, 40 days after tasseling has a large effect on corn. The control effect of spot disease was 80.8%, which was significantly higher ...

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Abstract

The invention relates to bacillus amyloliquefaciens and application thereof. The invention aims to solve the problems of the existing chemical agent for controlling leaf blight of corn that the environment is polluted, pathogenic bacteria easily generate drug resistance, and the yield increase effect of corn is not good. The bacillus amyloliquefaciens provided by the invention is bacillus amyloliquefaciens KB1-01 and is collected in China General Microbiological Culture Collection Center (CGMCC), the collection address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the collection date is September 12, 2014, and the collection number is CGMCC No.9649. The application of the bacillus amyloliquefaciens refers to application of the bacillus amyloliquefaciens in inhibition of leaf blight of corn and increase of yield of corn. By using the bacillus amyloliquefaciens, the control effect on the leaf blight of corn is 80.8%, and the yield increase rate of corn is 18.26%. The bacillus amyloliquefaciens is applied to the agricultural field.

Description

technical field [0001] The invention relates to a strain of bacillus amyloliquefaciens and its application. Background technique [0002] Corn spot disease is also known as streak disease, coal spot disease, dead leaf disease, leaf spot disease and so on. Mainly damage the leaves, leaf sheaths and bracts of corn. Water-soaked blue-gray spots first appear on the leaves, and then expand along the veins to both ends, forming large spots with dark brown edges, light brown or blue-gray centers. Late lesions often split longitudinally. In severe cases, the lesions merge and the leaves turn yellow and die. When wet, there are a large number of gray-black mold layers on the lesions. The lower leaves are affected first. On single-gene disease-resistant varieties, it shows as chlorotic lesions, small lesions, parallel to the veins, yellow-green or light brown in color, dark brown around, and some appear as necrotic spots. [0003] The control methods of maize leaf spot mainly in...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01P3/00A01P21/00C12R1/07
Inventor 刘洪亮王慧于琳
Owner 刘洪亮
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