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Artificial inoculation method for corn northern leaf blight of greenhouse facilities

A technology of maize large spot disease and artificial inoculation, which is applied in the fields of botanical equipment and methods, gardening methods, gardening tools/equipment, etc., can solve problems such as poor disease effect, and achieve the effect of improving disease effect and broad application prospect.

Active Publication Date: 2018-12-21
INST OF PLANT PROTECTION FAAS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the characteristics of temperature change in southern my country and the temperature and humidity environment conditions suitable for corn growth and the occurrence of maize leaf spot, the present invention utilizes the temperature adjustment, heat preservation and moisturizing effects of the facility greenhouse to reduce the temperature in areas with high corn earing stage. The method of scientific late sowing of autumn planting corn was chosen, and through the improvement of artificial inoculation technology under greenhouse conditions, an indoor artificial inoculation method for corn spot disease in greenhouse facilities was proposed. This method can ensure normal growth and earing of corn while ensuring The artificial inoculation of maize spot disease can achieve an ideal and stable incidence effect, and solve the problem that the artificial inoculation effect of maize spot disease is poor due to the high temperature during the heading period of corn in some warm regions in southern my country

Method used

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  • Artificial inoculation method for corn northern leaf blight of greenhouse facilities
  • Artificial inoculation method for corn northern leaf blight of greenhouse facilities
  • Artificial inoculation method for corn northern leaf blight of greenhouse facilities

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Infection effect of susceptible maize varieties in a greenhouse in Fuzhou area after being inoculated with large leaf spot

[0023] 1. The tested strain: Bacillus maize FJNJ20171103 (obtained from field disease samples);

[0024] 2. Test medium: Potato glucose medium (PDA medium): 200g potato, 20g glucose, 17g agar powder, 1000mL water, pH 6.8-7.0;

[0025] 3. Corn varieties susceptible to large spot disease for testing: Jinhuangtian No. 1 and Shangpin.

[0026] 4. Test method:

[0027] (1) Corn planting: in greenhouses in Fuzhou area (such as figure 1 ), sowed on September 9, 2017 (about 30 days later than the normal sowing time of local autumn corn). The vegetable garden soil with good water permeability was used as the planting soil. When the corn seedlings grew to 3 leaves and 1 heart on September 29, they were transplanted at a row spacing of 35×40 cm per hole, and two corn seedlings of the same variety were planted in each hole. normal fertilizer a...

Embodiment 2

[0045] Example 2 Determination of disease resistance of different maize varieties

[0046] 1. Corn varieties for testing: Jinhuangtian No. 1, Shangpin, Yuetian No. 16, Suyunuo 901 and Yongzhen No. 7;

[0047] 2, test method: with embodiment 1.

[0048] 3. Results and analysis

[0049] Table 3 The incidence of different maize varieties after adopting the method of the present invention to inoculate Pseudomonas spp.

[0050]

[0051] As shown in Table 3, the disease index of the five tested maize varieties ranged from 5.28 to 26.06, among which the disease index of the above product, Jinhuangtian 1, and Yuetian 16 were significantly higher than those of Suyunuo 901 and Yongzhen The disease index of No. 7 shows that after adopting the method of the present invention to inoculate the Pseudomonas spp., the resistance of different maize varieties to Pseudomonas spp. can be clearly distinguished.

Embodiment 3

[0052] Example 3 Effects of moisturizing treatment of plants in greenhouses on the pathogenicity after inoculation of P. maize at the end-heart and seedling stages

[0053] 1. The tested corn variety: Jinhuangtian No. 1;

[0054] 2. Test method: On November 29 in the greenhouse, inoculate the corn at the end of the heart leaf stage (sowing time is September 9) and the 6-7 leaf seedling stage (sowing time is October 15). Four groups were designed for the moisturizing treatment of the plants after inoculation, namely, 48 hours of film moisturizing + 7 days of spraying, 9 days of spraying, 48 hours of film moisturizing, and 48 hours of film moisturizing. Investigate the condition. The amount of bacteria sprayed on corn plants at the seedling stage of 6-7 leaves is 20mL / plant, see Example 1 for other methods.

[0055] 3. Result analysis:

[0056] Table 4 Effect of plant moisturizing treatment on the incidence of maize leaf spot

[0057]

[0058] The results showed that the ...

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Abstract

The invention provides an artificial inoculation method for corn northern leaf blight of greenhouse facilities. The method comprises the three steps of planting management of corns in a greenhouse, inoculation treatment of northern leaf blight of corn plants, and management of corn plants after inoculation, wherein under the greenhouse conditions of facilities, autumn corns with the delayed sowingtime being 20 to 30 days are selected as inoculation objects, at the late whorl stage of corns, exserohilum turcicum conidial suspension obtained after cultivating for 1 to 2 days on a PDA culture medium is adopted for spray inoculation, and the moisturizing conditions of the inoculated plants are optimized, so that on the condition of ensuring the normal growth of corn plants, indoor artificialinoculation of corn northern leaf blight reaches an ideal and stable incidence result. The method solves the problem that in some warm southern areas of China, due to the high temperature in the cornearing stage, the incidence result of artificial inoculation of corn northern leaf blight is poor.

Description

technical field [0001] The invention relates to an artificial inoculation method for fungal diseases of crops, in particular to an artificial inoculation method for maize leaf spot in greenhouse facilities. Background technique [0002] Corn has a long history of cultivation in my country and is highly adaptable. It can be grown in temperate, subtropical, and tropical regions. The planting area extends from Heilongjiang Province in the north to Hainan Province in the south. It is the second largest planting area in my country after rice and wheat. Cultivated crops. Umbilocystis maculatum ( Exserohilum turcicum ) caused by maize leaf spot is an important disease in maize production. [0003] Maize spot disease is a medium-low temperature disease, and its occurrence requires high environmental temperature and humidity. When the ambient temperature is 20-25°C and the relative humidity is above 80%, the disease develops rapidly and is prone to large-scale outbreaks. When the te...

Claims

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Application Information

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IPC IPC(8): A01G22/20A01G7/06
CPCA01G7/06A01G22/20
Inventor 杨秀娟代玉立甘林阮宏椿石妞妞杜宜新陈福如
Owner INST OF PLANT PROTECTION FAAS
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