Orchid aseptic sowing and test tube seedling propagation method and broad-spectrum culture medium adopted

A technology of aseptic seeding and culture medium, applied in botany equipment and methods, horticultural methods, applications, etc., can solve the problems of slow propagation speed, seeds without endosperm, random harvesting and excessive digging, etc., and achieve good growth and quality of seedlings Good, fast germination effect

Inactive Publication Date: 2011-12-21
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to long-term and uncontrolled digging, most orchid species are endangered, and all wild orchid species are protected by the "Convention on International Trade in Endangered Species of Wild Fauna and Flora" (CITES) and trade is restricted or prohibited
Orchids can

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) select the basically ripe and uncracked seeds of the vigorous white flower Phalaenopsis (Phalaenopsis amabilis) when blooming, soak the explants with 70% alcohol for 60 seconds, and then sterilize with 0.1% mercuric chloride solution for 20 minutes, After rinsing with sterile water for 4 times, the fruit was cut open, and the powdered seeds were inoculated on the seed germination medium for cultivation. The seeds germinated to form protocorms in 15 days. At 40 days, the protocorms differentiated into leaves. The seed germination medium contains 1 g of Huabao No. 1 per liter, 0.5 g of peptone, 50 mL of coconut milk, 0.5 g of activated carbon, 80 mg of inositol, 1.5 mg of glycine, 0.05 mg of thiamine hydrochloride (VB1), and pyridoxine hydrochloride (VB6) 0.4mg, niacin 0.4mg, naphthaleneacetic acid (NAA) 0.2mg, sucrose 15g, agar 7g, pH 5.4;

[0021] (2) Transfer the seedlings differentiated from the protocorm by seed germination to the same medium for cultivation, an...

Embodiment 2

[0026] (1) Select the basically mature and uncracked seeds of the robust Aerides rosea during flowering, soak the explants in 75% alcohol for 45 seconds, and then sterilize them with 0.15% mercuric chloride solution for 15 minutes , after rinsing with sterile water for 5 times, cut the fruit, inoculate the powdered seeds on the seed germination medium and cultivate them, and the seeds germinate to form protocorms in 10 days. At 30 days, the protocorms differentiated into leaves. Described seed germination medium contains Huabao No. 1 1.5g per liter, peptone 1g, coconut juice 75mL, gac 0.75g, inositol 100mg, glycine 2mg, thiamine hydrochloride (VB1) 0.1mg, pyridoxine hydrochloride ( VB6) 0.5mg, niacin 0.5mg, naphthaleneacetic acid (NAA) 0.5mg, sucrose 20g, agar 6.5g, pH 5.5;

[0027] (2) Transfer the seedlings differentiated from the protocorm by seed germination to the same medium for cultivation, and 1-2cm high seedlings can be formed in 30 days. The medium used was contain...

Embodiment 3

[0032] 1. (1) Select the basically mature and uncracked seeds of the robust rhizome Paphiopedilum rhizomatosum when blooming, soak the explants with 80% alcohol for 30 seconds, and then disinfect them with 0.2% mercuric chloride solution for 10 After rinsing with sterile water for 6 times, the fruit was cut open, and the powdered seeds were inoculated on the seed germination medium for cultivation, and the seeds germinated to form protocorms in 30 days. At 60 days, the protocorms differentiated into leaves. Described seed germination medium contains Huabao No. 1 2g per liter, peptone 2g, coconut juice 100mL, gac 1g, inositol 120mg, glycine 2.5mg, thiamine hydrochloride (VB1) 0.2mg, pyridoxine hydrochloride (VB6 ) 0.8mg, niacin 0.8mg, naphthaleneacetic acid (NAA) 2mg, sucrose 30g, agar 6g, pH 5.6;

[0033] (2) Transfer the seedlings differentiated from the protocorm by seed germination to the same medium for cultivation, and 1-2cm high seedlings can be formed in 50 days. The ...

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PUM

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Abstract

The invention discloses a breeding method for aseptic seeding and test tube seedling formation of orchids and the adopted broad-spectrum culture medium. In this method, a healthy orchid mother plant is selected, and artificially pollinated when it blooms, and the fruit that develops to maturity after pollination and has not cracked is used as an explant, or the orchid fruit that is basically mature and not cracked is taken from the wild as an explant. Implants undergo reproductive steps such as aseptic sowing, seed germination, strong seedling cultivation, and test-tube seedling transplantation. The specially prepared medium is used for aseptic sowing and seed germination to obtain small plants, and then the small plants are cultivated on the specially prepared strong seedling medium, and the seedlings are hardened under natural light in the greenhouse for 7-14 hours before being released from the bottle. One day, the test-tube seedlings were taken out, the root medium was washed off, and they were planted in a mixed substrate of bark, bluestone and peat, and further cultivated into seedlings. The invention has the characteristics of high seed germination rate, fast seedling growth, good seedling quality, low cost, etc., can obtain a large number of test-tube seedlings in a short period of time, and the transplanting survival rate of the seedlings can be kept above 90%. An effective way can be provided for the production of orchid seedlings.

Description

technical field [0001] The invention relates to a breeding method for aseptic sowing and test tube seedling formation of orchids. Background technique [0002] Orchids are elegant, beautiful and mysterious plants that are widely distributed in various terrestrial ecosystems except the poles and extremely arid desert regions, among which the tropical and subtropical regions are the most abundant. There are about 800 genera and 30,000 to 35,000 species of orchids in the world, which is one of the largest families in angiosperms. my country has a vast territory, superior climate resources, and rich orchid resources. It is known that there are more than 1,300 species of 173 genera, which are mainly distributed in the Yangtze River Basin and the provinces to the south. Due to its unique ornamental value and long flowering period, orchid is one of the most widely cultivated and sold flowers in the world. At present, the annual consumption of orchids in the world has exceeded 3 bi...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 段俊曾宋君吴坤林陈之林张建霞
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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