Orchid aseptic sowing and test tube seedling propagation method and broad-spectrum culture medium adopted

Abstract

The invention discloses a breeding method for aseptic seeding and test tube seedling formation of orchids and the adopted broad-spectrum culture medium. In this method, a healthy orchid mother plant is selected, and artificially pollinated when it blooms, and the fruit that develops to maturity after pollination and has not cracked is used as an explant, or the orchid fruit that is basically mature and not cracked is taken from the wild as an explant. Implants undergo reproductive steps such as aseptic sowing, seed germination, strong seedling cultivation, and test-tube seedling transplantation. The specially prepared medium is used for aseptic sowing and seed germination to obtain small plants, and then the small plants are cultivated on the specially prepared strong seedling medium, and the seedlings are hardened under natural light in the greenhouse for 7-14 hours before being released from the bottle. One day, the test-tube seedlings were taken out, the root medium was washed off, and they were planted in a mixed substrate of bark, bluestone and peat, and further cultivated into seedlings. The invention has the characteristics of high seed germination rate, fast seedling growth, good seedling quality, low cost, etc., can obtain a large number of test-tube seedlings in a short period of time, and the transplanting survival rate of the seedlings can be kept above 90%. An effective way can be provided for the production of orchid seedlings.

Description

technical field [0001] The invention relates to a breeding method for aseptic sowing and test tube seedling formation of orchids. Background technique [0002] Orchids are elegant, beautiful and mysterious plants that are widely distributed in various terrestrial ecosystems except the poles and extremely arid desert regions, among which the tropical and subtropical regions are the most abundant. There are about 800 genera and 30,000 to 35,000 species of orchids in the world, which is one of the largest families in angiosperms. my country has a vast territory, superior climate resources, and rich orchid resources. It is known that there are more than 1,300 species of 173 genera, which are mainly distributed in the Yangtze River Basin and the provinces to the south. Due to its unique ornamental value and long flowering period, orchid is one of the most widely cultivated and sold flowers in the world. At present, the annual consumption of orchids in the world has exceeded 3 bi...

AI Technical Summary

Problems solved by technology

However, due to long-term and uncontrolled digging, most orchid species are endangered, and all wild orchid species are protected by the "Convention on International Trade in Endangered Species of Wild Fauna and Flora" (CITES) and trade is restricted or prohibited

Orchids can usually be propagated by division or cuttings, but the propagation speed is slow; although the number of seeds in orchid fruits is large, because the seeds have no endosperm, they need to symbiosis with fungi in the wild to germinate, and the germination rate is extremely low