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Diterpene compound extracted from branches and leaves of croton and use thereof

A technology for compounds and diterpenoids, applied in the field of diterpenoids, can solve the problems that have not yet been reported in the literature, and achieve the effect of clear action material basis, favorable for export and foreign exchange earning, and easy to accept.

Inactive Publication Date: 2011-05-11
马英
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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Method used

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  • Diterpene compound extracted from branches and leaves of croton and use thereof
  • Diterpene compound extracted from branches and leaves of croton and use thereof
  • Diterpene compound extracted from branches and leaves of croton and use thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Get 100kg of croton branches and leaves, add 90% ethanol to reflux and extract 2 times, each 1 hour, add 800kg90% ethanol for the first time, add 600kg90% ethanol for the second time, filter, combine the extracts, the extracts reclaim the ethanol and Concentrate to the dilute extract with a relative density of 1.05, pass the dilute extract through D101 macroporous adsorption resin, first elute with 30% ethanol until it is colorless, discard the 30% ethanol eluate, and then use 90% ethanol Elute until colorless, collect the 90% ethanol eluate, recover the ethanol, and obtain the extract. Add this extract to a silica gel column with silica gel as a sample, chloroform-methanol gradient elution, collect the eluate, detect it with thin-layer chromatography, and combine it to obtain two parts A and B. Part A is passed through Sephadex LH-20 gel column, eluted with methanol, collect the eluate, identify by thin layer chromatography, combine, recover methanol, and then pass thr...

Embodiment 2

[0032] Take 200g of the 12-O-Tigloylphorbol-13-(2-methylbutyrate), add 200ml of absolute ethanol to dissolve, add 50ml of Tween-80 and glycerin and 1000ml of sterile water, mix well, and use the existing emulsion The preparation process is to prepare the pesticide containing the emulsion of 12-O-Tigloylphorbol-13-(2-methylbutyrate).

Embodiment 3

[0034] Take 200g of the 12-O-Acetylphorbol-13-(2-methylbutyrate), add 200ml of absolute ethanol to dissolve, add 50ml of Tween-80 and glycerin and 1000ml of sterile water, mix well, and use the existing emulsion The preparation process is to prepare the pesticide containing the emulsion of 12-O-Acetylphorbol-13-(2-methylbutyrate).

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PUM

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Abstract

The invention discloses a diterpene compound extracted from branches and leaves of croton. The diterpene compound comprises 12-O-Tigloylphorbol-13-(2-methy lbutyrate) and 12-O-Acetylphorbol-13-(2-methy lbutyrate). The invention further discloses the use of the diterpene compound in preparing biopesticide for killing tobacco mosaic virus and phytopathogen. Experiments prove that the diterpene compound extracted from branches and leaves of croton, including the 12-O-Tigloylphorbol-13-(2-methy lbutyrate) and the 12-O-Acetylphorbol-13-(2-methy lbutyrate), can remarkably stop tobacco mosaic virus from invasion and proliferation and restrain cucumber wilt disease fungus, wheat scab pathogen, magnaporthe grisea, thanatephorus cucumeris, bipolaris maydis, exserohilum turcicum and other phytopathogens.

Description

Technical field [0001] The invention belongs to the technical field of biopesticides, and relates to diterpenoids extracted from croton branches and leaves, and also relates to the application of the diterpenoids. Background technique [0002] At present, my country's agriculture is facing increasingly fierce international competition. In terms of agricultural product trade, strict and harsh pesticide residue standards are becoming a "green barrier" for my country's agricultural product export; on the other hand. With the continuous improvement of people's living standards, edible " "Pollution-free" agricultural and sideline products have also become a must for the people of the country. Compared with chemical pesticides, biopesticides have the advantages of being safe to humans, animals and non-target organisms, good environmental compatibility, not easy to develop resistance, easy to protect biodiversity, and have a wide range of sources. Therefore, the development and app...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/013C07C69/33C07C67/56A01N37/06A01N37/02A01P1/00A01P3/00
Inventor 马英
Owner 马英
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