Bacillus amyloliquefaciens preparation and application thereof
A technology of amylolytic spores and bacillus, applied in the direction of application, fungicides, biocides, etc., can solve the problems of ineffective increase in corn production, easy resistance of pathogens, and unsafe conditions for humans and animals, and achieve good control effects and long-lasting effects The effect of lengthening and reducing the protrusion rate
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specific Embodiment approach 1
[0008] Specific embodiment one: a kind of Bacillus amyloliquefaciens preparation of this embodiment comprises 0.01~0.02% zinc sulfate by mass fraction, 0.01~0.02% boric acid, 0.1~0.2% Tween 20 and the Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) of balance KB1-01 fermented liquid, wherein the component content per 1 liter of the fermentation medium for preparing Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) KB1-01 fermented liquid is: glucose 0.1%, soybean powder 0.4%, peptone 0.1%, yeast extract 0.05% , 0.12% potassium dihydrogen phosphate, 0.024% magnesium sulfate and the rest of water; the fermentation conditions are: temperature 32°C, pH 7.0-7.2, rotation speed 170r / min, incubation time 48h, inoculum size 2%.
[0009] The invention uses Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) KB1-01 to prepare a high-efficiency, broad-spectrum, non-toxic and pollution-free ecological biocontrol microbial agent, which is friendly to the environment and do...
specific Embodiment approach 2
[0010] Specific embodiment two: the difference between this embodiment and specific embodiment one is that the bacillus amyloliquefaciens preparation comprises 0.02% zinc sulfate by mass fraction, the bacillus amyloliquefaciens (Bacillus amyloliquefaciens) of 0.02% boric acid, 0.1% Tween 20 and 99.86% KB1-01 fermented liquid, others are identical with specific embodiment one.
specific Embodiment approach 3
[0011] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that: the Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) KB1-01 is preserved in the General Microorganism Center of China Committee for the Preservation of Microorganisms, and the preservation address is Beijing No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, the preservation date is September 12, 2014, and the preservation number is CGMCCNo.9649. Others are the same as the specific implementation mode 1 or 2.
[0012] The screening method of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) KB1-01 of the present embodiment is: collect corn rhizosphere soil sample 15 from Heilongjiang Province Agricultural Reclamation Harbin, Hongxinglong, Mudanjiang, Jiusan, Suihua, Bei'an, Baoquanling seven sub-bureaus share.
[0013] The collected soil samples were air-dried naturally. Weigh 10g of each soil sample, put it into a triangular flask fille...
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