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Bacillus amyloliquefaciens preparation and application thereof

A technology of amylolytic spores and bacillus, applied in the direction of application, fungicides, biocides, etc., can solve the problems of ineffective increase in corn production, easy resistance of pathogens, and unsafe conditions for humans and animals, and achieve good control effects and long-lasting effects The effect of lengthening and reducing the protrusion rate

Active Publication Date: 2015-12-30
刘洪亮
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems that the existing chemical agents in the prevention and treatment of large spot of corn pollute the environment, are unsafe for humans and animals, the bacteria are prone to drug resistance, and the effect on increasing corn production is not obvious, and a kind of Bacillus amyloliquefaciens is provided Preparations and their applications

Method used

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  • Bacillus amyloliquefaciens preparation and application thereof
  • Bacillus amyloliquefaciens preparation and application thereof
  • Bacillus amyloliquefaciens preparation and application thereof

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specific Embodiment approach 1

[0008] Specific embodiment one: a kind of Bacillus amyloliquefaciens preparation of this embodiment comprises 0.01~0.02% zinc sulfate by mass fraction, 0.01~0.02% boric acid, 0.1~0.2% Tween 20 and the Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) of balance KB1-01 fermented liquid, wherein the component content per 1 liter of the fermentation medium for preparing Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) KB1-01 fermented liquid is: glucose 0.1%, soybean powder 0.4%, peptone 0.1%, yeast extract 0.05% , 0.12% potassium dihydrogen phosphate, 0.024% magnesium sulfate and the rest of water; the fermentation conditions are: temperature 32°C, pH 7.0-7.2, rotation speed 170r / min, incubation time 48h, inoculum size 2%.

[0009] The invention uses Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) KB1-01 to prepare a high-efficiency, broad-spectrum, non-toxic and pollution-free ecological biocontrol microbial agent, which is friendly to the environment and do...

specific Embodiment approach 2

[0010] Specific embodiment two: the difference between this embodiment and specific embodiment one is that the bacillus amyloliquefaciens preparation comprises 0.02% zinc sulfate by mass fraction, the bacillus amyloliquefaciens (Bacillus amyloliquefaciens) of 0.02% boric acid, 0.1% Tween 20 and 99.86% KB1-01 fermented liquid, others are identical with specific embodiment one.

specific Embodiment approach 3

[0011] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that: the Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) KB1-01 is preserved in the General Microorganism Center of China Committee for the Preservation of Microorganisms, and the preservation address is Beijing No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, the preservation date is September 12, 2014, and the preservation number is CGMCCNo.9649. Others are the same as the specific implementation mode 1 or 2.

[0012] The screening method of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) KB1-01 of the present embodiment is: collect corn rhizosphere soil sample 15 from Heilongjiang Province Agricultural Reclamation Harbin, Hongxinglong, Mudanjiang, Jiusan, Suihua, Bei'an, Baoquanling seven sub-bureaus share.

[0013] The collected soil samples were air-dried naturally. Weigh 10g of each soil sample, put it into a triangular flask fille...

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Abstract

A bacillus amyloliquefaciens preparation and its application. The invention relates to a bacillus amyloliquefaciens preparation and its application. The invention aims to solve the problem that an existing chemical agent for controlling northern leaf blight of corn pollutes the environment, is not safe to human and livestock, makes germs easily generate drug resistance and has unconspicuous corn yield-increasing effects. The bacillus amyloliquefaciens preparation contains, by weight, 0.01-0.02% of zinc sulfate, 0.01-0.02% of boric acid, 0.1-0.2% of Tween 20 and the rest of bacillus amyloliquefaciens KB1-01. The application of the bacillus amyloliquefaciens preparation is an application of the bacillus amyloliquefaciens preparation in inhibiting northern leaf blight of corn and increasing corn yield. Control effect on northern leaf blight of corn is 80.8%, and corn yield-increasing rate is 18.26%. The invention is applied in the field of agriculture.

Description

technical field [0001] The invention relates to a bacillus amyloliquefaciens preparation and application thereof. Background technique [0002] Corn spot disease is also known as streak disease, coal spot disease, dead leaf disease, leaf spot disease and so on. Mainly damage the leaves, leaf sheaths and bracts of corn. Water-soaked blue-gray spots first appear on the leaves, and then expand along the veins to both ends, forming large spots with dark brown edges, light brown or blue-gray centers. Late lesions often split longitudinally. In severe cases, the lesions merge and the leaves turn yellow and die. When wet, there are a large number of gray-black mold layers on the lesions. The lower leaves are affected first. On single-gene disease-resistant varieties, it shows as chlorotic lesions, small lesions, parallel to the veins, yellow-green or light brown in color, dark brown around, and some appear as necrotic spots. [0003] The control methods of maize leaf spot mai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P3/00A01P21/00A01N63/00A01N59/14
Inventor 刘洪亮王慧于琳
Owner 刘洪亮
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