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Pseudomonas fluorescens CB113 QS system comQ gene deletion mutant strain and application thereof

A technology of Pseudomonas fluorescens and mutant strains, which is applied in the field of microorganisms to achieve a good effect of inhibiting fungi

Inactive Publication Date: 2014-05-21
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the quorum sensing mechanism produced by each Gram-negative bacteria is different, its regulatory proteins are highly homologous, and most of the Gram-negative bacteria currently studied have similar QS systems

Method used

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  • Pseudomonas fluorescens CB113 QS system comQ gene deletion mutant strain and application thereof
  • Pseudomonas fluorescens CB113 QS system comQ gene deletion mutant strain and application thereof
  • Pseudomonas fluorescens CB113 QS system comQ gene deletion mutant strain and application thereof

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Experimental program
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Effect test

Embodiment

[0020] 1. Cloning the comQ gene from the genomic DNA of the CB113 strain

[0021] Primers were designed for the conserved sequence of comQ in bacteria related to the CB113 strain, and the comQ gene sequence of the strain was obtained by PCR. The gene comQ was located and cloned from CB113 strain by comparing the genome sequences and analyzing its function. The full sequence of comQ is as follows:

[0022] 1 atgaaggaga ttgtaagcca aaaaataatg aatcaggact tggaacgata tcttcagaat

[0023] 61 tttattgagt ctaaggatac gtttgagttt gccgatttgg ctcttcatca ttatttagct

[0024] 121 tttaacggtc aggaccaaaa agcaatcgaa ttgctagctg ccggaattga attgcttata

[0025] 181 ctctcattcg atatttatga tgacttagag gatcaagata atggaagtgc tgtctggatg

[0026] 241 aagattgact cgtcaatcgc tttaaatgca gttacagctc tttacaccct aagtatacaa

[0027] 301 gtgatgtgtc aagctagtca tgaaccagaa tttacacaag agatattgaa ttttgcgtta

[0028] 361 caatcaattc aaggccagca tgatgatatt gtgaatgcgc cgcaaactga agaagcctgt

[0029] 421 cttgaaatga tcaaaaa...

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Abstract

The invention provides a pseudomonas fluorescens CB113 QS system comQ gene deletion mutant strain and application thereof, and belongs to the technical field of microorganisms. The pseudomonas fluorescens mutant strain M2 is obtained due to the deletion of comQ gene coding sequence 675bp in pseudomonas fluorescens CB113. Compared with the wild type strain, the pseudomonas fluorescens mutant strain M2 has an advantage in application that the inhibitory activity to pathogenic fungus consisting of exserohilum turcicum and cylindrocarpon destructans is obviously improved. The strain provided by the invention shows the characteristics of relatively high fungus inhibiting characteristics and brings a good way for inhibiting fungus for the prior art.

Description

technical field [0001] The invention relates to a strain of Pseudomonas fluorescens, in particular to a mutant strain of Pseudomonas fluorescens with comQ gene deletion, and belongs to the technical field of microorganisms. Background technique [0002] Bacterial quorum sensing (QS) is an important mechanism for bacteria to communicate intracellularly or intercellularly and coordinate group behavior according to the population density. Although the quorum sensing mechanism produced by each Gram-negative bacteria is different, its regulatory proteins are highly homologous, and most of the Gram-negative bacteria currently studied have similar QS systems. In Pseudomonas fluorescens, comX-comQ-comP-comA is the main signaling pathway in the QS system, which regulates the formation of bacterial competent cells, the production of spores, the formation of biofilms, and the production of antibiotics. The comX gene encodes a 55-amino acid comX pheromone precursor, and comQ is respons...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/21A01N63/00A01P3/00C12R1/39
Inventor 张祥辉王璐潘洪玉赵玉海王秀明
Owner JILIN UNIV
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