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One strain forming a block type fanjun fungus, fermentation culturing method and application thereof

A technology of Pantoea agglomeration and fermentation culture, applied in the field of Pantoea agglomerans and its fermentation culture, can solve the problems of reducing disease prevention effect and human health threat, and achieves good disease prevention effect, high action efficiency and biological safety. high effect

Inactive Publication Date: 2006-09-13
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Frequent use of antibiotics can lead to resistance of pathogens, reduce the effectiveness of disease prevention, and also pose a threat to human health

Method used

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  • One strain forming a block type fanjun fungus, fermentation culturing method and application thereof
  • One strain forming a block type fanjun fungus, fermentation culturing method and application thereof
  • One strain forming a block type fanjun fungus, fermentation culturing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1. Isolation, identification and preservation of Pantoea agglomerans B501 CGMCC No. 1655

[0031] 1. Isolation and screening of strains with good antagonistic effect on pathogens

[0032] Pathogen:

[0033] Alternaria alternata is isolated from the surface of the black spot of winter jujube fruit in the field. After purification, it is inoculated on PDA medium (200g potato, 15g glucose, 15g agar, dilute to 1000mL with water), and cultivated at 28℃ for 10-14d Then, with the help of a hemocytometer, the concentration is about 1×10 with sterile water 6 Spore mL -1 The spore suspension.

[0034] fruit:

[0035] Winter jujubes were harvested in Zhanhua, Shandong Province, China. After pre-cooling for 2 days, they were put into PVC bags and kept in refrigerator at -2°C. Jujube fruit hardness 12.53kg / cm 2 , The soluble solid content is 36.8%, and the total acid is 1.2%. Before inoculation, the winter jujube was sterilized with 2% NaOCl for 2 minutes, washed with distilled ...

Embodiment 2

[0057] Example 2: Pantoea agglomerans (Pantoea agglomerans) B501 CGMCC No. 1655 fermentation culture and bacterial preparation

[0058] Pantoea agglomerans (Pantoea agglomerans) B501 CGMCC No. 1655 Erlenmeyer flask, seed tank special liquid medium formula is: tryptone 10g, yeast extract 5g, sucrose 15g, NaCl 10g, dilute to 1000mL with water.

[0059] Pantoea agglomerans (Pantoea agglomerans) B501 CGMCC No.1655 fermenter special liquid medium formula is: sucrose 10g, tryptone 5g, corn flour 20g, soybean flour 20g, wheat bran 20g, Na 2 HPO 4 2g, NaH 2 PO 4 0.2g, MgSO 4 0.3g, ZnSO 4 0.5g, CaCO 3 2g, soak enemy 0.1g, dilute to 1000mL with water.

[0060] 1. Activation of strains

[0061] Pick the original species of Pantoea agglomerans B501 CGMCC No. 1655, inoculate them in LB liquid medium, and culture them on a shaker at 28°C for 1-2 days.

[0062] 2. Triangular flask culture

[0063] The Pantoea agglomerans B501 CGMCC No. 1655 activated in step 1 was inoculated into an Erlenmeyer f...

Embodiment 3

[0069] Example 3. Biocontrol effect detection of Pantoea agglomerans B501 CGMCC No. 1655

[0070] Taking the black spot of jujube, apple gray mold, penicillium, soft rot of pear and green mold of citrus as examples, the detection of Pantoea agglomerans B501 CGMCC No.1655 (hereinafter referred to as "CGMCC No.1655") The control effect of Pantoea agglomerans B501) on plant diseases, the specific detection methods are as follows:

[0071] 1. Detection of the inhibitory effect of Pantoea agglomerans B501 on jujube black spot

[0072] 1. Detect the growth dynamics of Pantoea agglomerans B501 on winter jujube wounds

[0073] Many fruit diseases invade from wounds, so the effective colonization ability of Pantoea agglomerans B501 on winter jujube wounds is a necessary condition to determine its control effect. Four treatments were designed in this experiment: Pantoea agglomerans B501 (1×10 9 cfumL -1 )+Alternaria (the pathogen of jujube fruit black spot disease, 1×10 6 Spore mL -1 ); Pan...

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Abstract

The invention discloses Pantoea agglomerans B50l CGMCC No.1655 and fermenting cultivating method and application. The method includes the following steps: inoculating the Pantoea agglomerans B50l CGMCC No.1655 into triangle bottle to gain seed liquid, inoculating the seed liquid into fermenting jar special cultivating medium for 48-96 hours. It has good effect to avoid disease, high effect efficiency, high biology safety and simple manufacture technology.

Description

Technical field [0001] The invention relates to a strain of Pantoea agglomerans and its fermentation culture method and application. Background technique [0002] In 1989, Gavini et al. (Gavini, F.et al. Transfer of Enterobacter agglomerans (Beijerinck 1888) Ewing and Fife 1972 to Pantoea gen.nov.as Pantoeaagglomerans comb.nov.and description of Pantoea dispersa sp.nov.[J]. Int. J. Syst. Bacteriol, 1989, 39: 337-345.) created the Genus Pantoea (Genus Pantoea), which includes two species: Pantoea agglomerans and Pantoea dispersa. Among them, Pantoea agglomerans is synonymous with Enterobacter agglomerans, Erwinia herbicola and Erwinia milletiae, which are carotenoid-producing The yellow flora was first isolated from plants and seeds, and the flora was later found in a variety of environments. Researchers have conducted a lot of research work on Pantoea agglomerans and found that it has important roles in medicine, agriculture and genetics, mainly in the following aspects: [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02
Inventor 薛梦林
Owner NORTHWEST A & F UNIV
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