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Ginseng endophytic paenibacillus polymyxa and application thereof

A polymyxa spore-like and ginseng technology, applied to ginseng endophytic Paenibacillus polymyxa and its application field, can solve problems such as continuous cropping obstacles, and achieve the effects of strong bacteriostatic ability and broad bacteriostatic spectrum

Active Publication Date: 2013-08-28
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The strain can effectively solve the continuous cropping obstacles of old ginseng land, ginseng, American ginseng and other plants

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: the isolation of endophytic Paenibacillus polymyxa in ginseng of the present invention

[0014] Pick healthy ginseng plants from old ginseng fields, wash the soil on the surface of fresh ginseng with tap water, rinse it with sterile water several times, dry it with sterile filter paper, soak it in 75% ethanol for 1 min, and rinse it with sterile water 4 times, soak in 5% sodium hypochlorite solution for 5 minutes, rinse 4 times with sterile water, then soak in 75% ethanol for 30 seconds, rinse 4 times with sterile water, and dry the remaining water on the surface of ginseng with sterile filter paper. Collect the washing solution of the last washing of ginseng, take 200 μl and spread it on the PDA plate medium, culture it in an incubator at 25°C for two days, observe whether there are colonies on the plate, and use it as a blank control. Cut the aseptically treated ginseng into thin slices of about 1cm×1cm with a sterile scalpel blade, then place it on a PD...

Embodiment 2

[0015] Example 2 Identification of endophytic Paenibacillus polymyxa in ginseng of the present invention

[0016] 1. Morphological characteristics, Gram staining and spore staining identification of endogenous Paenibacillus polymyxa in ginseng

[0017] The colony characteristics, Gram staining and spore staining identification methods of endogenous Paenibacillus polymyxa in ginseng mainly refer to "Berger's Bacterial Identification Handbook" edited by R.E. Buchanan et al. The identification results are as follows: After the bacterium was cultured on LB medium for 24-48 hours at 25°C, the colonies were milky white, opaque, small, mostly 0.5-1mm in diameter, irregularly round or oval, and the surface was not smooth. Raised in the middle, ragged edges, tacky, and unpigmented. After being cultured at 25°C for 24-48 hours on PDA medium, the colony is colorless and transparent, with a diameter of 0.8-1.5mm, round, smooth surface, raised, neat edges, moist and sticky, no pigment pr...

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Abstract

The invention relates to the technical field of biology, and specifically discloses a ginseng endophytic paenibacillus polymyxa and application thereof. The ginseng endophytic paenibacillus polymyxa was preserved in General Microorganism Center of China Microorganism Culture Preservation Management Committee on February 5, 2013. The address is No.3 No. 1 Court, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology Chinese Academy of Sciences. The preservation number is CGMCC No. 7250. The ginseng endophytic paenibacillus polymyxa is a paenibacillus polymyxa strain isolated and identified from a ginseng plant, can be widely and directly applied in plant disease control of ginseng, American ginseng, schisandra chinensis, fritillary bulb, and astragalus, can be applied in the preparation of preventions for treatment of diseases of ginseng, American ginseng and other plants, and can effectively solve the successive cropping obstacle problems of old ginseng grounds, and ginseng, American ginseng and other plants.

Description

technical field [0001] The invention relates to a ginseng endogenous Paenibacillus polymyxa and its application, belonging to the field of biotechnology. Background technique [0002] Ginseng and American ginseng are Araliaceae plants, which are world-renowned precious medicinal plants. The chemical components include ginsenosides, sugars, amino acids, vitamins, proteins, polypeptides, organic acids, fat-soluble components, and nearly 40 kinds of inorganic elements. Clinical and laboratory studies have shown that it has good pharmacological effects in regulating immunity, anti-stress and fatigue, anti-oxidation, anti-inflammation, anti-tumor, lowering blood sugar, and protecting the liver. [0003] Root disease is one of the main factors restricting the yield and quality of ginseng and American ginseng. In my country, the annual yield loss caused by root disease can reach 20%-50%. Weakened the competitiveness of Chinese ginseng and American ginseng products in the internati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00A01P3/00C12R1/01
Inventor 郜玉钢张连学臧埔卢宗志祝彦海李学刘群赵雪亮李丽刘彤
Owner JILIN AGRICULTURAL UNIV
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