Plant tissue culture bacteriostatic (antiseptic) agent containing botanical fungicide

A botanical fungicide and plant tissue culture technology, applied in the field of plant tissue culture, can solve problems such as weak research on botanical fungicides, achieve great application and promotion value, environmental and human and animal safety, and reduce or prevent fungal and bacterial contamination Effect

Inactive Publication Date: 2012-01-11
TIANJIN BINCHENG LONGDA GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the research on botanical insecticides has been more in-depth, including insecticidal plant resources and distribution, insecticidal active ingredients, mode of action, mechanism of action, and commercialization; while research on botanical fungicides is relatively weak

Method used

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  • Plant tissue culture bacteriostatic (antiseptic) agent containing botanical fungicide
  • Plant tissue culture bacteriostatic (antiseptic) agent containing botanical fungicide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Induction of embodiment 1 'green apple' arrowroot shoot tip bunched buds

[0066] (1) Induction of clustered buds (this step is implemented under aseptic conditions in Example 1):

[0067] (1) Get the young side buds or terminal buds of 'green apple' arrowroot plants as explants;

[0068] (2) Soak the lateral buds or terminal buds in detergent liquid for 10 minutes, and rinse them with running water for 20 minutes;

[0069] (3) On the ultra-clean workbench, it can be soaked for 10 minutes with 10% bacteriostatic (killing) agent of the present invention, then treated with 75% alcohol for 15 seconds, 0.1% mercuric chloride for 7 to 8 minutes, and rinsed with sterile water for 5 minutes. Immerse in sterile water for later use;

[0070] (4) Cut off redundant bracts with a sterilized scalpel on the pretreated side buds or terminal buds, leaving only smaller shoot tips and bud tips, which are inoculated on the induction medium containing bacteriostatic agents. Cultivate an...

Embodiment 2

[0077] The strong seedling rooting culture of embodiment 2 Green Apple' arrowroot cluster buds

[0078] Embodiment 2 is to implement under relatively less sterile conditions. In this step, glass bottles and plastic cups (bottles, boxes) are used as culture containers, the culture medium can save the high-pressure sterilization procedure, the inoculation can not need ultra-clean workbench, and it is separated from the strict aseptic operating environment, which simplifies the process of plant tissue culture and seedling cultivation , Improve work efficiency and greatly reduce the cost of seedling cultivation.

[0079] (1) Prepare rooting medium for strong seedlings containing bacteriostatic agents: After the culture medium is boiled and dissolved, add 2.0ml / 1L of hormones and bacteriostatic agents for strong seedlings of plants to take root in the medium, and then divide them into washed and Dry culture contents (such as: glass bottles, plastic cups, plastic bottles, plastic b...

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Abstract

Provided is a plant tissue culture bacteriostatic (antiseptic) agent containing botanical fungicide. The bacteriostatic (antiseptic) agent comprises isothiazolinone, nisin and aqueous extracts of the Chinese herbal medicines of terminalia, groundsel and aromatic madder. When the bacteriostatic (antiseptic) agent is applied in plant tissue culture, 0.35 to 0.5% of the bacteriostatic (antiseptic) agent is added into a medium, which enables fungal and bacterial contamination to a culture to be effectively reduced or prevented and poses little adverse influence on plant growth, thereby enhancing the success rate of plant tissue culture; the bacteriostatic (antiseptic) agent can also be applied in open type plant culture, e.g., in the rooting phase of sound seedlings during a late plant culture stage, the bacteriostatic (antiseptic) agent is directly added into a medium, and the medium can be inoculated in indoor environment with bacteria or a few bacteria without undergoing the step of autoclaving or operating on a superclean bench, thereby simplifying seedling culture in plant tissue culture, enhancing working efficiency, saving a great amount of energy and reducing production cost for plant tissue culture seedlings; therefore, the bacteriostatic (antiseptic) agent has a critical actual application value.

Description

technical field [0001] The invention belongs to the application technology of plant tissue culture in the field of biotechnology, and relates to a plant inhibitor ()bactericide containing a botanical fungicide suitable for plant tissue culture, which can effectively reduce or prevent fungal and bacterial pollution. It can be widely used in the fields of plant biotechnology such as rapid propagation of plant tissue culture, establishment of transgenic and regenerated plant systems, and the like. Background technique [0002] The factory production of plant tissue culture seedlings is developing rapidly in our country, and the techniques of tissue culture are becoming more and more perfect. However, different degrees of contamination often occur in tissue culture. It is understood that many scholars have difficulty obtaining sterile vaccines at the stage of primary culture, or although sterile vaccines have been obtained in primary culture, a large amount of contamination or ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01N65/22A01N43/80A01N43/90A01N65/12A01N65/08A01P1/00A01P3/00
Inventor 夏时云张友强李雁鹏邢洁吴金凤宫宇李楠
Owner TIANJIN BINCHENG LONGDA GROUP
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