4results about How to "Solve build problems" patented technology

This invention discloses a method for controlling the oxygen partial pressure and performance of barium titanate ceramics during sintering using coconut shells, comprising the following steps: Step 1: Select coconut shells, clean them, remove the fibers, and dehydrate and dry them using a high-temperature forced-air drying oven; Step 2: Crush the dried coconut shells into small pieces, place them in a crucible, and carbonize them at high temperature in a muffle furnace; Step 3: Take out the high-temperature carbonized coconut shell char, grind it, and sieve it; Step 4: Weigh the coconut shell char and barium titanate powder at a mass ratio of 2~6:100, and then grind them to fully mix the coconut shell char and barium titanate powder; Step 5: Add a binder to the mixed powder, grind it thoroughly, and then press the powder into sheets to obtain a green body of fully mixed coconut shell char and barium titanate; Step 6: Place the pressed green body into a muffle furnace and sinter it at high temperature; Step 7: Obtain the coconut shell char mixed barium titanate ceramic.

The invention relates to a construction method of an OriCiro cell-free cloning system based on an RCR technology, and belongs to the technical field of molecular biology. The construction method comprises the following steps: expression and purification of high-activity Tn5 transposase, transposon-mediated oriC insertion, RCR amplification, traceless excision of oriC transposon and repair cyclization. The construction method provided by the invention provides a rapid, reliable and mutation-burden-free new genome construction and rescue path for the existing research, can solve the difficult problem of construction of large-size and toxic DNA sequence vectors for gene therapy, shortens the research and development and production periods, and improves the production efficiency. Therefore, a powerful tool is provided for the fields of preparation of mRNA vaccines or therapy plasmids, construction of gene therapy vectors, supply of pharmaceutical-grade plasmid DNA, research of a virus molecular mechanism and the like.

The utility model discloses an elastic glue filling device. The filling device comprises a storage piece, a conveying piece, a conveying channel, a pressure adjusting piece and an air pressure control assembly, the storage part is used for storing elastic glue with compressibility; the conveying part is arranged at the bottom of the material storage part, communicates with the material storage part and is used for pressurizing and outputting the elastic rubber; one end of the conveying channel is connected with the output side of the conveying part, and the other end of the conveying channel is at least partially arranged above the storage part and used for enabling the elastic glue to flow back to the storage part; a filling part is arranged in the middle of the conveying channel and is used for filling and outputting the elastic glue; the pressure adjusting part is arranged at the end, away from the conveying part, of the conveying channel. The air pressure control assembly is connected with the pressure adjusting piece and used for providing constant air pressure for the pressure adjusting piece. Through the arrangement, the perfusion stability and precision of the perfusion device are higher.

The utility model provides a positive pressure type anti-foaming solution transfer device, which belongs to the technical field of chemical fluid conveying equipment and comprises a conveying pipe, an air inlet valve and a soft self-locking plug, the soft self-locking plug is provided with a liquid flow channel penetrating through the upper end face and the lower end face, and the conveying pipe is placed in the liquid flow channel. Meanwhile, the two ends of the conveying pipe penetrate through the upper end face and the lower end face of the soft self-locking plug respectively and are arranged outside the soft self-locking plug, the portion, arranged on the upper portion of the soft self-locking plug, of the conveying pipe is a liquid outlet pipe, the portion, arranged on the lower portion of the soft self-locking plug, of the conveying pipe is a liquid inlet pipe, and an air inlet valve is further installed at the upper end of the soft self-locking plug. The device disclosed by the utility model can effectively solve the problem of foam generation in the transfer process of easy-to-foam media such as polymer solutions, surfactants and the like, and has the characteristics of compact structure, strong compatibility and safety in operation.