Sfp gene-deleted biocontrol bacteria as well as fermentation technology and application thereof

Abstract

The invention provides bacillus amyloliquefaciens HAB-2, which has the collection number of CCTCC M 2015070. Although the key lipopeptide synthetase gene sfp gene is deleted, the surfactin substances can still be generated, indicating that the strain has a specific and distinctive biosynthetic pathway and has a remarkably high research value. The bacillus amyloliquefaciens HAB-2 has very strong ability of inhibiting pathogenic fungi, has a wide antibacterial spectrum and can inhibit multiple kinds of pathogenic fungi while the antibacterial effect is good and the antibacterial rate is high. Further research finds that the active ingredient can be directly obtained through simple treatment on the fermentation broth of the strain, and the antibacterial active ingredient is stable and has very high activity in strong acid and strong alkaline environments; the antibacterial activity is not changed after 24 hours of ultraviolet irradiation, and high temperature does not influence the antibacterial active ingredient; and the fermentation broth of the biocontrol bacteria realizes certain growth promoting effect on a plant.

Description

[0001] technical field [0002] The invention belongs to the field of microorganisms, and in particular relates to a biocontrol bacterium lacking an sfp gene and its fermentation process and application. Background technique [0003] Bacillus is a kind of Gram-positive strain that produces spores. It is widely distributed, rich in physiological characteristics, easy to isolate and cultivate, and is an important microbial flora in nature. Bacillus can produce a variety of antibacterial active substances, including Non-ribosomal peptide antibiotics synthesized by ribosomal pathway and bacteriocins, enzymes and other active protein antibacterial substances synthesized by ribosomal pathway. [0004] Strain 168 is the model strain of Bacillus and the most thoroughly studied strain at present. Its whole genome was sequenced in 1997. Different from the wild Bacillus, the model strain 168 lost many wild-type characteristics due to the long-term subculture in the laboratory, such ...

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Problems solved by technology

Different from the wild Bacillus, the model strain 168 lost many wild-type characteristics due to the long-term subculture in the laboratory, such as the spontaneous mutation of the sfp gene, which made the 168 strain lose the production of lipopeptides The ability of similar compounds, thus lacking the ability of antibacterial

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