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Nonabsorbent streptomyces ahygroscopicus strain BWL58 for preventing and treating banana vascular wilt and application thereof

A technology for streptomyces hygroscopicus and banana wilt, applied in the direction of application, bacteria, and fungicides, can solve the problems of affecting the effect of biocontrol and weak antagonistic ability of pathogenic bacteria, and achieve good control effect, good development and application prospects, and cultivation conditions simple effect

Inactive Publication Date: 2012-01-25
HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the biological control measures for banana wilt that have been reported or applied for patents use Bacillus as a biocontrol strain. These strains have many advantages as biocontrol bacteria, but there is also a serious deficiency, that is, the antagonistic ability of the strain itself to pathogenic bacteria Not strong, which may affect its biological control effect

Method used

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  • Nonabsorbent streptomyces ahygroscopicus strain BWL58 for preventing and treating banana vascular wilt and application thereof
  • Nonabsorbent streptomyces ahygroscopicus strain BWL58 for preventing and treating banana vascular wilt and application thereof
  • Nonabsorbent streptomyces ahygroscopicus strain BWL58 for preventing and treating banana vascular wilt and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Isolation and identification of strains

[0031] The biocontrol bacterium among the present invention is a strain of Streptomyces screened and isolated from the virgin forest soil of Bawangling, Hainan Island, which has a strong inhibitory effect on Fusarium oxysporum f.sp.cubense. On May 9, 2011, it was deposited in the General Microorganism Center (CGMCC) of China Committee for Culture Collection of Microbial Cultures (CGMCC), the preservation number is CGMCC No.4837, and the classification name is Streptomyces ahygroscopicus.

[0032] The isolation and screening process of bacterial strain BWL58 is as follows: the plant rhizosphere soil is collected from Bawangling virgin forest in Hainan Island, then the soil sample is air-dried, diluted with sterile water to an appropriate multiple, and spread on the screening medium. The screening medium is Add potassium dichromate, nalidixic acid and cycloheximide PDA solid medium, culture at 28°C for more than 5 days, after the ...

Embodiment 2

[0036] Liquid bacterial agent and preparation method thereof

[0037] The liquid microbial agent containing the above-mentioned bacterial strain BWL58 provided in this example is prepared by the following method:

[0038] (1) strain activation, the bacterial strain BWL58 is inoculated on the solid medium, and the medium is a nutrient agar medium;

[0039] (2) Seed culture, inoculate the activated bacteria into the liquid medium, and the formula is: 2% glucose, 1% soybean cake powder, 0.2% yeast extract, 0.3% NaCl, CaCO 3 0.1%, K 2 HPO 4 0.05%, the rest is water; pH value 7.0-7.2; under 28 ℃, 160 rev / min shake flask fermentation and culture in Erlenmeyer flask for 72 hours. After the seed cultivation process, the liquid fermentation can be carried out directly;

[0040] (3) Liquid fermentation, the seed liquid is added to the liquid medium by 6% (volume percentage content), and the formula is: glucose 2%, soybean cake powder 1%, soluble starch 2%, yeast extract 0.2%, NaCl 0...

Embodiment 3

[0042] Solid bacterial agent and preparation method thereof

[0043] The solid microbial agent containing the above bacterial strain BWL58 provided in this example is prepared by the following method:

[0044] (1) Strain activation: inoculate the bacterial strain BWL58 on a solid medium, and the medium is a nutrient agar medium;

[0045] (2) Seed culture: Inoculate the activated strain into the liquid medium, and the formula is: 1% glucose, 1% soybean cake powder, 0.2% soybean peptone, 0.2% yeast extract, 0.3% NaCl, CaCO 3 0.1%, K 2 HPO 4 0.05%, the rest is water, what adopts in the present embodiment is preferred culture medium, other culture medium also can, there is no special restriction; pH value 7.0-7.2; Under 28 ℃, triangular flask 160 rev / min shakes the flask Ferment and cultivate for 72 hours. In fact, if there are enough strains prepared in step (1), you can directly carry out solid fermentation without going through the seed cultivation process;

[0046] (3) So...

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Abstract

The invention discloses a nonabsorbent streptomyces strain BWL58 for preventing and treating a banana vascular wilt. The strain BWL58 is classified and named as nonabsorbent streptomyces ahygroscopicus, and is preserved in the China General Microbiological Culture Collection Center on May 9, 2011; the preservation number is CGMCC No. 4837; the strain has strong antagonistic capability to the pathogenic bacteria of the banana vascular wilt and can be used for preventing and treating the banana vascular wilt; the invention further discloses a microbial inoculum containing the strain, a preparation method and application thereof; and by using the microbial inoculum, not only can the banana vascular wilt be inhibited, but also the microbial inoculum has a certain growth promotion effect on a plant.

Description

technical field [0001] The invention relates to a bacterial strain for preventing and treating banana wilt and its application, in particular to a non-hygroscopic streptomyces strain BWL58 for preventing and controlling banana wilt and its application. Background technique [0002] Banana wilt is a devastating soil-borne disease in banana production caused by Fusarium oxysporum f.sp.cubense infection. There are 4 physiological races of this pathogenic bacteria, and the two physiological races No. 1 and No. 4 are distributed in my country. The No. 4 physiological race is the most harmful to the banana industry in my country. It has been found in Hainan, Guangdong, Yunnan, and Fujian. Part of the banana planting area occurred on a large scale, and the affected area has exceeded 500,000 mu. In Guangdong and Hainan where the banana wilt disease is serious, it is no longer possible to grow bananas on a large scale and have to switch to other crops. [0003] Banana Fusarium wilt ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/465
Inventor 曾涛曾会才陈汉清莫坤联李戈
Owner HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
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