Wettable powder of streptomyces roseoflavus biological bactericide

A technology of Streptomyces roseus flavum and biological fungicides, applied in the direction of fungicides, biocides, chemicals for biological control, etc., can solve the problems of high cost, large unit dosage, long time, etc., to reduce packaging and The effect of transportation cost, reduced unit consumption, and strong resistance to adversity

Inactive Publication Date: 2013-06-05
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the defects of the above two patent applications are: in the previous patent, the use of microbial secondary metabolites as pesticides or their lead compounds requires the exact structure of the secondary metabolites to be clarified
The secondary metabolites of the strain play a major role in the fermentation broth of the 0116 strain, and the cost and cycle of separating, purifying and identifying the components of the fermentation broth are relatively high and the cycle is long, so chemical synthesis cannot be carried out, nor can it mass production
At the same time, since the main component of the fermentation liquid is water, the unit consumption of the fermentation liquid directly applied to the field is large, resulting in high packaging and tr

Method used

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  • Wettable powder of streptomyces roseoflavus biological bactericide
  • Wettable powder of streptomyces roseoflavus biological bactericide
  • Wettable powder of streptomyces roseoflavus biological bactericide

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Embodiment Construction

[0040] Step 1: After culturing the 0116 strain on PDA medium at 28° C. for 4-5 days, inoculate it into the seed medium at a ratio of 1%. The culture condition is: 28° C. / 200 r, culture for 3 days.

[0041] The seed medium is: 3% peanut cake powder, 4.5% starch, 1.5% glucose, 0.75% sodium chloride, 0.3% alcohol master paste, 0.9% ammonium sulfate, 0.03% potassium dihydrogen phosphate, 0.375% calcium carbonate, 0.105% Ferrous sulfate, 0.105% zinc sulfate, 3% sucrose, 0.3% fructose, pH natural;

[0042] Step 2: Mix the seed culture solution and diatomaceous earth at a ratio of 1.5:1, put it in a 18×24cm disk for solid fermentation at 28°C for three days, turn the disk once a day, and dry it after three days;

[0043] Step 3: Dry the above solid fermentation product in a forced air drying oven under the following conditions: 35°C, 72-120h.

[0044] Step 4: add 4% sodium lignin sulfonate to the dried fermentation product, mix thoroughly, and then pass through an AO type airflow m...

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Abstract

The invention discloses wettable powder of a streptomyces roseoflavus biological bactericide, which adopts diatomite as a matrix and comprises the following components: not less than 108 propagules of streptomyces roseoflavus strain 0116, and 4% of a wetting dispersant. It is determined that diatomite is used as a carrier and sodium lignosulphonate is used as the wetting dispersant. In addition, the strain wettable powder storage stability is determined by regular detection of the viable bacterial count, and results show that after strain wettable powder storage for 24 months, the viable bacterial count is more than 80%. The invention has the characteristics of short development period, low cost, simple process, and capability of being developed into various dosage forms, and is suitable for large-scale production. Since propagules of streptomyces roseoflavus strain 0116 is used as the main component of the wettable powder, the invention is suitable for large-scale production, and when compared with strain fermentation liquor, the invention greatly shortens the field using amount, and reduces packaging and transport cost.

Description

Technical field: [0001] The invention relates to a diatomite-based Streptomyces roseus flavum biological fungicide wettable powder for preventing and controlling plant diseases. Background technique [0002] At present, the prevention and control of plant diseases is mainly based on chemical pesticides. Their long-term use has caused great selection pressure on pathogenic bacteria and easy to produce drug resistance. Moreover, the high residue and high pollution of chemical pesticides have a serious impact on the environment and the safety of people and animals. Therefore, bio-pesticides with low toxicity and low residue have become a major trend in the development and production of pesticides. Hebei Agricultural College Students' Defense Office applied for two patents on biocontrol preparations on November 2, 2005 and June 9, 2009, namely "a biological fungicide and its preparation method, application number / patent number: 200510117421" And "a biological fungicide wettabl...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01N25/14A01P3/00
Inventor 李亚宁刘大群赤国彤李爱霞孟庆芳张立荣李星张娜张汀
Owner HEBEI AGRICULTURAL UNIV.
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