Application of yingluo mycin in preparing drug resisting plant pathogenic fungi

A technology of enromycin and antimycin, which is applied in the field of application of enluomycin in the preparation of anti-phytopathogenic fungal drugs, can solve the problems that the anti-fungal activity of the same compound has not been seen, and achieve significant anti-phytopathogenic fungi. The effect of fungal action

Inactive Publication Date: 2010-05-05
MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, apart from this article, there have been no relevant reports on the antifungal activity of compounds with the same structure as engromycin

Method used

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  • Application of yingluo mycin in preparing drug resisting plant pathogenic fungi
  • Application of yingluo mycin in preparing drug resisting plant pathogenic fungi
  • Application of yingluo mycin in preparing drug resisting plant pathogenic fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] : the fermentation of englomycin producing bacteria

[0032] (yeast extract 0.4%, malt extract 1%, glucose 0.4%, agar 1.2%, pH7.2) streptomyces strain Streptomyces albidoflavus I07A-0182 grown on the slant was inoculated in the seed medium (glucose 0.5%, yeast extract 0.5%) ,, peptone 0.5%, beef extract 0.5%, corn steep liquor 0.4%, starch 2%, soybean cake powder 1%, CaCO 3 0.4%, CoCl 2 0.002%, pH 7.2) 50ml / 250ml shake flask, cultured on a rotary shaker at 28°C for 36hrs, then transferred to a 1L / 5L fermentation medium (the formula is the same as the seed medium) 1L / 5L shake flask with a 5% inoculation amount , cultivated on a rotary shaker at 28° C. for 72 hours, and harvested a total of 40 L of fermentation broth.

Embodiment 2

[0033] : the extraction of Yingluomycin

[0034] After centrifugation (4000r / min, 20min) of 40 liters of fermentation broth of Streptomyces albidoflavus I07A-0182, the supernatant was extracted with an equal volume of ethyl acetate, and the organic phase was washed with anhydrous Na 2 SO 4 Dehydration and concentration under reduced pressure gave about 20 g of an oily tan crude extract.

Embodiment 3

[0035] : the separation of Enromycin

[0036] The above crude extract was dry-loaded on a forward silica gel chromatography column (250g, 100-200 mesh, column volume 3x40cm), using cyclohexane-ethyl acetate 19:1, 4:1, 2:1, 1:1 500 ml each, graded elution, activity tracking showed that cyclohexane-ethyl acetate 2:1 part showed strong activity against rice blast fungus.

[0037] The cyclohexane-ethyl acetate 2:1 part was concentrated under reduced pressure to obtain about 1 g of the yellow crude product, which was applied to a reverse silica gel chromatography column (50 g, 40-60 μm, column volume 1×50 cm) by wet method, and methanol- Water carries out graded gradient elution (40% methanol 100ml, 60% methanol 300ml, 75% methanol 400ml, 80% methanol 300ml, 90% methanol 200ml), activity tracking shows that 75% methanol part has stronger activity against rice blast.

[0038] Merge the main active fractions, remove methanol by rotary evaporation, freeze-dry to obtain semi-pure prod...

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Abstract

The invention relates to an application of yingluo mycin in preparing drug resisting plant pathogenic fungi, wherein yingluo mycin is natural source antimycin antibiotics (Antimycin) and is obtained by separating Streptomyces albidoflavus I07A-01824 in fermentation liquor. Experiments prove that the compound has obvious function of resisting plant pathogenic fungi, can be further developed and researched into a high-efficient agricultural antibiotics and can be applied to the fields, such as crop cultivation, food refreshment and the like.

Description

Technical field: [0001] The invention relates to the application of a natural source antimycin antibiotic (Antimycin) in the preparation of medicines against plant pathogenic fungi. Background technique: [0002] Fungi are the most important group of plant pathogens, and about 70% of plant diseases are caused by fungi. During the long-term use of chemical pesticides to control fungal diseases, three major problems have arisen: environmental pollution, increased resistance of pathogenic bacteria, and imbalance of microbial flora in plants. Due to the increasingly prominent side effects of chemical pesticides and the increasing demand for environmental protection and green food, the research and development of biological pesticides that meet the concepts of environmental protection, health and sustainable development has become a top priority. [0003] Agricultural antibiotics have attracted much attention due to their advantages of high efficiency, easy decomposition, no res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/08C07D321/00A01N43/24A01P3/00C12R1/465
Inventor 孙承航韩宁宁闫蕾蕾张玉琴余利岩金文藻
Owner MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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