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Preparation and use of lilac grey streptomycete and active product thereof

A technology of Streptomyces lilacaceae and antibacterial active substances, which is applied in the field of preparation of Streptomyces lilacaceae and its active products, can solve the problems that the potential has not been fully developed and applied, and achieve low cost, stable product quality, The effect of easy operation

Inactive Publication Date: 2009-07-15
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current market share of biopesticides in my country is only 2-3%, and its potential is far from being fully developed and applied.

Method used

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  • Preparation and use of lilac grey streptomycete and active product thereof
  • Preparation and use of lilac grey streptomycete and active product thereof
  • Preparation and use of lilac grey streptomycete and active product thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment one: Screening and identification of bacterial strain xjy

[0036] Through the antibacterial test in the dish, 6 strains were screened out from 51 strains of soil actinomycetes to have inhibitory activity against tomato leaf mold (Fulviafulva), among which strain xjy had the best inhibitory effect. The antimicrobial spectrum of strain xjy was measured and found that its sterile fermentation filtrate had different degrees of inhibitory effect on 23 common plant pathogenic fungi and 6 kinds of bacteria ( figure 1 , A: tomato leaf mold B: wheat sheath blight C: kiwifruit dry blight D: pear rot fungus. ), and the bacterial strain xjy has good subculture antibacterial stability, and can produce β-1,3-glucanase and protease; through the morphological characteristics of the bacterial strain xjy ( figure 2 A: spore; B: hyphae), cultural characteristics, physiological and biochemical characteristics, analysis of cell wall components and 16SrDNA homology sequence ana...

Embodiment 2

[0037] Embodiment two: the preparation method of bacterial strain xjy antibacterial active substance

[0038]First, vibrate on the seed medium (5g peptone per liter, 3g yeast extract, 2g glucose, 1g beef extract, pH7.0, liquid volume 40mL / 250mL) at 28°C, 80r / min for 2 days to make seed liquid; then Add 10% seed liquid in the fermentation medium (every liter contains 2% soybean powder, 2% glucose, 0.8% NaCl, CaCO 3 0.2%, (NH 4 ) 2 SO 4 0.32%, pH 7.0, liquid volume 40mL / 250mL) at 28°C, 180r / mim shake flask culture for 6 days, the fermentation broth was centrifuged twice at 13000rpm for 20min each time to obtain the sterile supernatant.

Embodiment 3

[0039] Embodiment three: the property and structural analysis of the active substance produced by bacterial strain xjy

[0040] Through the stable determination of the sterile supernatant after the fermentation of bacterial strain xjy, it was found that the active substance produced by bacterial strain xjy had better thermal stability ( image 3 , ①A: 50°C 30min; ①B: 50°C 60min; ①C: 50°C 90min; ①D: 50°C 120min; ①E: 50°C 150min; ①F: sterile supernatant; ②A: 100°C 30min; ; C: 90min at 100°C; D: 120min at 100°C; E: 150min at 100°C; F: sterile supernatant), acid-base stability and light UV stability, which are alkaline water-soluble substances; the fermentation of xjy strain Use oxalic acid to adjust the pH value of the sterile supernatant to 2 and precipitate overnight. After suction filtration, use an equal volume of acetone to incubate in a water bath at 50°C for 1 hour. After the acetone is removed by filtration and concentration, the pH is adjusted to 7, and the pretreated an...

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Abstract

The invention belongs to the technical field of biological pesticides, discloses a Streptomyces l avendulae xjy strain which has been collected in the general microorganism center of CCCCM in 8.16.2007 with the collection number: CCMCC No.2130. The invention also discloses a separation and authentication method for the strain, a preparation method for antibiotic active substances of the Streptomyces l avendulae xjy strain, authentication for analysis structures of the active substances, prevention and cure experiments for metabolic active substances of the xjy strain against leaf muld of tomato, powdery mildew of cucumber and phytophthora blight of pepper, and growth-promoting experiments for the growth of the strain. The antibiotic active substances secreted by Streptomyces xjy strain is mainly nucleosides antibiotic having the characteristics of persistent effect and no medicinal hazard as well as having the effects of obviously preventing and curing vegetable diseases and promoting the growth to a certain extent.

Description

technical field [0001] The invention belongs to the technical field of biopesticides, and in particular relates to a preparation method and application of a novel Streptomyces lilacinus griseus and its active product. Background technique [0002] Due to the long-term use of chemical pesticides, plant diseases have adaptive variation to fungicides. Under the long-term selection pressure of some fungicides, obvious resistance can occur, resulting in the failure of chemical control. The application strategies of different fungicides should be considered comprehensively in production, so as to give full play to the effects of various fungicides and avoid the emergence of drug resistance as much as possible. Since Waksman and Henrici established the genus Streptomyces in 1943, Streptomyces has become the genus with the largest number of actinomycetes and the largest variety of antibiotics, with more than 1,000 species and varieties. [0003] As an important group of biopesticid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04C07D405/04A01N63/02A01N43/40A01P3/00A01P21/00C12R1/56
Inventor 黄丽丽姜云康振生何其瑞
Owner NORTHWEST A & F UNIV
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