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Bacterial-wilt-resistant Streptomyces rochei strain and bio-organic fertilizer thereof

A technology for Streptomyces louchei and bacterial wilt, which is applied in the biological field and can solve problems such as food safety, unstable effects, and environmental pollution

Inactive Publication Date: 2016-09-28
GUIZHOU TOBACCO SCI RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commonly used strategies to control bacterial wilt include chemical pesticides, soil improvement, disease-resistant varieties, grafting and crop rotation, etc., but the effects are not stable
In addition, environmental pollution and food safety problems caused by excessive application of chemical pesticides have aroused widespread concern and concern

Method used

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  • Bacterial-wilt-resistant Streptomyces rochei strain and bio-organic fertilizer thereof
  • Bacterial-wilt-resistant Streptomyces rochei strain and bio-organic fertilizer thereof
  • Bacterial-wilt-resistant Streptomyces rochei strain and bio-organic fertilizer thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1 Isolation and identification of antagonistic bacteria L-9 of the present invention

[0015] 1.1 Isolation and screening of antagonistic bacteria L-9

[0016] The uninfected tobacco plants and rhizosphere soil were collected from many tobacco bacterial wilt disease-infected fields in Guizhou Province, and stored at low temperature for isolation of antagonistic bacteria. Tobacco plant sample processing: take rhizome tissue, first sterilize the surface with 2% sodium hypochlorite for 2 minutes, and then wash with sterilized deionized water for 3 to 5 times. For soil samples or treated tobacco plant samples, dilute the soil solution or plant sample solution to 10% according to the dilution coating plate method. -1 ~10 -6 stand-by.

[0017] Spread the soil solution or plant sample solution evenly on Gao’s No. 1 medium, and after cultivating for 24 hours at 30°C in the dark, spray the Ralstonia solanacearum suspension evenly on the plate after the colony grows...

Embodiment 2

[0057] Embodiment 2 The preparation of biological organic fertilizer of the present invention

[0058] The antagonistic bacteria L-9 was cultured in Gaoshi No. 1 liquid medium, and cultured in a shaker at 30° C. at 170 rpm for 48 hours. The organic fertilizer is a mixture of rapeseed meal organic fertilizer enzymatically hydrolyzed and cow dung compost at a weight ratio of 1:1. It has been determined that it contains 33.8% organic matter, 4.3% amino acids, N4.20%, P 2 o 5 2.26%, K2 O1.08%. Add antagonistic bacteria L-9 culture solution to the above-mentioned organic fertilizer at an inoculation weight of 5%, adjust the temperature to 30-45°C, and water content to 30-40%, and ferment for 4-5 days, during which it is constantly turned over. After the fermentation is completed, the water content reaches 34.9%, which is called bio-organic fertilizer (hereinafter referred to as "BIO").

Embodiment 3

[0059] Example 3 The effect of BIO on bacterial wilt resistance in seedling cultivation experiments

[0060] Test varieties: K326 and Honghua Dajinyuan.

[0061] Vermicompost nursery substrate: see patent ZL200710202167.6 for the preparation method.

[0062] 3.1 Experimental design

[0063] 3.1.1 The effect of BIO on the germination rate of tobacco

[0064] 100 K326 seeds or safflower Dajinyuan seeds per plate. The substrate is composed of conventional seedling substrates plus different proportions of vermicompost seedling substrates and BIO. The treatments are shown in Table 1.1. Spread about 1cm thick. Each treatment is repeated 5 times and cultivated under light. Cultivate in a box at 25°C and 75% water, and regularly measure the germination rate.

[0065] Table 1.1 Experimental design for the effect of BIO on tobacco seed germination

[0066]

[0067]

[0068] 3.1.2 Effect of BIO on tobacco seedling growth

[0069] According to the results of the influence of BI...

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Abstract

The invention discloses a bacterial-wilt-resistant antagonistic bacterium strain. The strain is named Streptomyces rochei L-9, and was collected by China General Microbiological Culture Collection Center on September 25th, 2013; and the collection number is CGMCC No.8264. The strain L-9 has high bacterial wilt resistance, and the antagonistic ring radius reaches 15mm. The invention provides a new microbial resource for biological control on bacterial wilt, and overcomes the defects in the prior art.

Description

technical field [0001] The invention belongs to the field of biological technology, in particular to an antagonistic bacteria against bacterial wilt, in particular to a strain of Streptomyces rochei L-9, and also relates to the application of the bacterial strain in resisting bacterial wilt. Background technique [0002] Tobacco bacterial wilt is one of the main diseases of tobacco in tropical and subtropical regions. Currently, it commonly occurs in the Yangtze River Basin and south of China, including Guangdong, Fujian, Taiwan, Hunan, Jiangxi, eastern Guangxi, southern Anhui, Sichuan and Local tobacco-smoking areas in Guizhou have caused serious damage, and outbreaks often occur in individual years, causing devastating losses. In recent years, the incidence and range of damage of the disease has a tendency to expand to the northern smoking areas, and it has occurred in Shandong, Henan, Shaanxi, Liaoning and other provinces, and the damage is serious in some areas. At pres...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01P1/00C05F15/00C05F17/00C12R1/465
CPCC05F3/00C05F11/08C12N1/205C12R2001/465C05F11/00C05F17/00Y02W30/40
Inventor 石俊雄刘艳霞李想孟琳蔡刘体张恒邹焱
Owner GUIZHOU TOBACCO SCI RES INST
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