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Method for increasing yield of lincomycin by co-expressing metK1 and metK2 genes in streptomyces lincolnensis

A lincomycin, co-expression technology, applied in the field of genetic engineering, can solve the problem of unclear influence on the yield of functional lincomycin

Inactive Publication Date: 2018-03-02
ANHUI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the functions of metK1 and metK2 in Streptomyces lincomyces and their effects on lincomycin production are still unclear.

Method used

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  • Method for increasing yield of lincomycin by co-expressing metK1 and metK2 genes in streptomyces lincolnensis
  • Method for increasing yield of lincomycin by co-expressing metK1 and metK2 genes in streptomyces lincolnensis
  • Method for increasing yield of lincomycin by co-expressing metK1 and metK2 genes in streptomyces lincolnensis

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Embodiment 1

[0029] 1.1 Strains, plasmids and growth conditions

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Abstract

The invention discloses a method for increasing the yield of lincomycin by co-expressing metK1 and metK2 genes in streptomyces lincolnensis. The method is characterized in that the metK1 and metK2 genes in the streptomyces lincolnensis are co-expressed through a genetic engineering way, so that an engineering strain with high lincomycin yield of the streptomyces lincolnensis is obtained; and the strain obtained by the technology can be fermented to increase the yield of the lincomycin.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a method for increasing the yield of lincomycin by co-expressing metK1 and metK2 genes in Streptomyces lincoeum. Background technique [0002] Streptomyces can produce abundant secondary metabolites, and these secondary metabolites and their derivatives are widely used, such as antibiotics, anticancer drugs, immunosuppressants, etc. More than half of the antibiotics and anticancer drugs currently used in medicine are produced by Streptomyces ] . Whole-genome sequencing studies have found that Streptomyces contains multiple biosynthetic gene clusters, and these gene clusters are either silent or have weak biosynthetic ability, and need to be screened to obtain high-yielding strains for industrial production. In the past, industrial production strains were mainly obtained by physical or chemical mutagenesis. Traditional mutagenesis techniques are not only time-consum...

Claims

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Application Information

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IPC IPC(8): C12N1/21C12P19/64C12R1/565
CPCC12N9/1085C12P19/64C12Y205/01006
Inventor 张部昌许玉荣柯美兰吴杭
Owner ANHUI UNIVERSITY
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