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Streptomyces lunalinharesii ZJNU968 strain and applications thereof

A technology of streptomyces and strains, applied in the field of biotechnology agricultural production, can solve problems affecting the control effect, polluting the environment, and pesticide residues

Active Publication Date: 2017-11-17
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical control is currently the most effective way to control tobacco red spot disease, but this method will cause pesticide residues, pollute the environment, and affect human health
In addition, chemical control will also make tobacco Alternaria resistance, which will affect the control effect

Method used

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  • Streptomyces lunalinharesii ZJNU968 strain and applications thereof
  • Streptomyces lunalinharesii ZJNU968 strain and applications thereof
  • Streptomyces lunalinharesii ZJNU968 strain and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Isolation and screening of Streptomyces lunarinharesii ZJNU968 strain from soil.

[0020] (1) Culture medium:

[0021] Gaoshi No. I liquid medium: soluble starch 20g, KNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 0.5g, NaCl0.5g, FeSO 4 0.01g, distilled water 1000ml, pH 7.2-7.5.

[0022] Potato dextrose agar medium (PDA): 200g potato, 20g glucose, 20g agar, 1000ml water, natural pH.

[0023] (2) Isolation and purification of actinomycetes strains

[0024] 16 soil samples were collected from Wucheng District, Jinhua City, Zhejiang Province, and the soil suspension was prepared by gradient dilution method, and 10 -3 、10 -4 、10 -5 Spread 0.1ml of the soil dilution solution on the Gaoshi No. 1 medium plate, let it stand at room temperature for 30 minutes, and then place it upside down in a constant temperature incubator at 28°C for cultivation. The outgrown actinomycetes were transferred to a new Gaulier I medium plate for further purification, and the finally obtaine...

Embodiment 2

[0031] The production method of embodiment 2 streptomyces Streptomyces lunarinharesii ZJNU968 strain fermented liquid:

[0032] Inoculate the Streptomyces lunarinharesii ZJNU968 strain preserved on the slant of the test tube onto the Gaoshi No. 1 medium plate, and then culture it at 28°C for 3 days; use a sterile puncher to punch out a 6mm diameter bacterial block on the plate with antagonistic bacteria , and wash the thalline with sterile water, insert it into a 250ml Erlenmeyer flask equipped with 30ml of Gaoshi No. 1 liquid medium, cultivate it in a shaker at a speed of 180r / min and a temperature of 28°C for 144h, and obtain Streptomyces lunarinharesiiZJNU968 strain after centrifugation fermentation broth.

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Abstract

The invention belongs to the field of biotechnological agricultural production, and particularly relates to a Streptomyces lunalinharesii ZJNU968 strain and applications thereof, wherein the strain is preserved in the China General Microbiological Culture Collection Center (the address is Institute of Microbiology, Chinese Academy of Sciences, 3# Court No.1, Beichen West Road, Chaoyang District, Beijing.) on November 11, 2013, and has the preservation number of CGMCC No:8465. According to the present invention, the Streptomyces lunalinharesii ZJNU968 strain and the fermentation broth thereof can be used for preventing and controlling tobacco brown spot.

Description

technical field [0001] The invention belongs to the field of biotechnology agricultural production, and in particular relates to Streptomyces lunalinharesii ZJNU968 strain and application thereof. Background technique [0002] Tobacco red spot (Alternaria alternata) is a fungal leaf spot disease in the mature stage of tobacco, which not only affects the yield of flue-cured tobacco, but also affects the smoking quality of tobacco leaves. The disease is more common in tobacco producing areas and is one of the most threatening diseases in tobacco production. Chemical control is currently the most effective method to control tobacco red spot disease, but this method will cause pesticide residues, pollute the environment and affect human health. In addition, chemical control will also make tobacco Alternaria resistance, which will affect the control effect. [0003] As people's requirements for the safety of tobacco and tobacco products are getting higher and higher, reducing t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/465
CPCA01N63/10C12N1/20C12N1/205C12R2001/465
Inventor 张艳军张萍华
Owner ZHEJIANG NORMAL UNIVERSITY
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