Fermenting method for producing natamycin

A technology for producing natamycin and a fermentation method, applied in the field of antibiotics, can solve the problems of high cost and long production cycle, and achieve the effects of low cost, short production cycle and stable output

Inactive Publication Date: 2007-01-10
广州市微生物研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The production of natamycin has existed before...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0021] Setting: 50-liter fermenter, seed tank culture inoculation volume is 5%, fermenter culture inoculation volume is 10%.

[0022] A. The fermentation medium formula (weight percentage) is as follows:

[0023] Nitrogen source uses peptone 0.8%

[0024] The carbon source is glucose 15%

[0025] Potassium salt selection KH 2 PO 4 0.1%

[0026] B. Fermentation process conditions

[0027] (1) Shake flask culture

[0028] The capacity of a 50-liter fermentation tank is 30 liters, so the seed tank seed volume is 30*10%=3 liters, and the shake flask seed volume is 3*5%=0.15 liters. Prepare 0.15 liters of culture medium according to the above formula, and pack them into 500ml Erlenmeyer flasks, each with a volume of 75ml. After packaging, use steam to sterilize, cool to 30℃ and insert the bacteria, the shaker speed is 180rpm, and the temperature is controlled at 30 ℃, pH 5.5, after 26h incubation, combine shake flask seeds.

[0029] (2) Seed pot culture

[0030] In...

example 2

[0034] Setting: 500 liters of fermentation tank, 300 liters of filling volume, 10% of seed tank culture inoculum, fermentation tank

[0035] The culture inoculum amount is 13%.

[0036] A. The fermentation medium formula (weight percentage) is as follows:

[0037] The nitrogen source uses yeast extract 0.6%

[0038] The carbon source is 1.2% sucrose

[0039] Potassium salt uses KCl 0.5%

[0040] B. Fermentation process conditions

[0041] (1) Shake flask culture

[0042] According to the setting, the seed tank seed volume is 300×13%=39 liters, and the shake flask seed volume is 39×10%=3.9 liters.

[0043] According to the medium formula, equipped with 3.9 liters of medium, divided into 500ml Erlenmeyer flasks, each bottle is 100ml, sterilized after packaging, cooled to 23℃ to insert the bacteria, the shaker speed is 280rpm, the culture temperature is 23℃, The pH value is 7.5. After 66 hours of culture, the seeds are combined and bottled.

[0044] (2) Seed pot culture

[0045] In a...

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PUM

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Abstract

The invention relates to the fermenting method for producing natamycin that breeds a streptomycete strain of high yield natamycin, by the method of fermenting metabolizing method to select the fermenting culture medium compounding and fermenting technology condition. The method has the advantages of high yield, low cost, short producing cycle, etc.

Description

Technical field [0001] The invention relates to a fermentation method for producing natamycin, belonging to the technical field of antibiotics. Background technique [0002] Natamycin, also known as Pimaricin, is an important polyene antibiotic. Its molecule is an active cyclic tetraene compound containing more than 3 crystal waters. Appearance is white or cream, it is a kind of tasteless crystalline powder, molecular formula is C 33 H 47 NO 13 , The molecular weight is 665.73. Natamycin is a strong antifungal agent, which can effectively inhibit the growth of yeast and mold and prevent the formation of aflatoxin in filamentous fungi. Natamycin is currently approved as a natural food preservative and widely used in the food industry. [0003] Natamycin has been produced before, but its production cycle is longer and the cost is higher. The natamycin produced by the fermentation culture method of the present invention has strong antibacterial ability, short production period, low ...

Claims

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Application Information

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IPC IPC(8): C12P19/62
Inventor 梁淑娃姚朔影杜少平李运南董加喜明飞平王丽霞李展鹏
Owner 广州市微生物研究所
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