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Streptomyces strain and application thereof

A technology of streptomyces and fungi, applied in the direction of application, bacteria, fungicides, etc., can solve problems such as soil decline, restrictions on the export of agricultural products, threats to people's quality of life, etc., and achieve the effects of promoting plant growth and increasing production, good prevention and control , Significant effect of disease prevention and seedling protection

Active Publication Date: 2009-09-09
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, excessive application and abuse of pesticides have also led to serious problems such as soil decline, environmental pollution, impact on non-target organisms and pest resistance, etc.
Especially in recent years, human and animal poisoning incidents caused by excessive pesticide residues in agricultural products have occurred continuously, seriously threatening the quality of life of the people and restricting the export of agricultural products in my country

Method used

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  • Streptomyces strain and application thereof
  • Streptomyces strain and application thereof
  • Streptomyces strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1. Isolation and identification of Streptomyces strain YL04

[0026] 1. Separation of strains

[0027] Streptomyces strain YL04 is a strain with potential biocontrol effect discovered during the strain isolation of potato early blight leaves collected from the potato base in Keshan County, Heilongjiang in 2004. The specific isolation steps are as follows:

[0028] 1. Cut and sample the part of the disease-health boundary, rinse with sterile water twice, soak in 70% alcohol for 5 seconds to disinfect the surface, rinse once with sterile water, and place it on sterile absorbent filter paper. Let dry.

[0029] 2. Inoculate the disinfected diseased samples on rye sucrose agar medium and incubate at 20°C for 7 days. Seven days later, it was found that while the hyphae of Phytophthora infestans grew on one of the media, another colony was formed. The medium around the colony can produce an obvious inhibition zone, which shows certain resistance to Phytophthora infestans. In...

Embodiment 2

[0084] Example 2. Screening of culture medium and culture conditions of strain YL04

[0085]There are many kinds of media for actinomycetes in common use, such as Gao's No. 1 medium, oatmeal agar medium, Cha's medium, etc., but for different species of actinomycetes and different culture purposes, the most suitable medium different. In this experiment, 44 kinds of culture medium were selected as the test medium, and 1-2 kinds of the most suitable medium for YL04's growth and production were screened out; at the same time, the most suitable bottling was determined when the strain YL04 was cultivated for the purpose of producing the protein. Amount, pH value, shaking speed and incubation time. It provides references for further research on the physicochemical properties, structural characteristics, mechanism of action and industrial fermentation production of antibacterial substances produced by YL04.

[0086] 1. Screening of growth medium

[0087] 1. Transfer the strain YL04 stored...

Embodiment 3

[0113] Example 3 Antibacterial activity of strain YL04 (measurement of antibacterial spectrum)

[0114] 1. The inhibitory effect of strain YL04 metabolite on pathogenic fungi

[0115] In this experiment, 15 species of phytopathogenic fungi from 13 genera were selected, and the Oxford cup method was used to detect the inhibitory effect of YL04 metabolite on pathogenic fungi, in order to clarify the antibacterial biocontrol effect and control spectrum of YL04 bacteria and metabolites. Provide a basis for further research and development and field application of this strain.

[0116] The tested pathogenic fungi are as follows: Pythium aphanidermatum, Phytophthora melonis, Phytophthora copsici, Geaumanomyces graminis, Valsa mali , Rhizoctonia solani, Rhizoctonia cerealisbv, Pyricularia grisea, Verticillium dahliae, Alternaria solani, Alternaria solani Exserohilum turcicum, Bipolaris maydis, Fusarium graminearum, Fusarium oxysporum f.sp. vasinfectum, Botrytis cinerea. The above pathoge...

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Abstract

The invention discloses a Streptomyces sp. strain and the application thereof. The strain provided by the invention is Streptomyces sp. YL04 CGMCC No.2884. The strain YL04 provided by the invention with ideal biocontrol effect and development value can be popularized in large-area fields and mainly solves the problem that increasingly serious damping-off and blight diseases of crop seedlings like vegetables and melons cause serious output reduction or reduced product quality. The invention has significant scientific meaning and application value in enriching strain resources for biocontrol of our country and provides effective measures for achieving high-output and high-quality modern agriculture. The invention meets the national standards of agricultural goods production safety and the need of constructing a non-polluted, green and organic agricultural production base and has certain economic and social benefits.

Description

Technical field [0001] The invention relates to a strain of Streptomyces and its application. Background technique [0002] For centuries, chemical pesticides have played an active role in the prevention and control of plant diseases, pests, weeds, and rodents, and have made important contributions to the stable production, increase in production, recovery of losses and improvement in quality of agriculture. However, excessive application and abuse of pesticides have also led to serious problems such as the decline of soil fertility, environmental pollution, and affecting the resistance of non-target organisms and pests. Especially in recent years, human and livestock poisoning incidents caused by excessive pesticide residues in agricultural products have occurred continuously, which has seriously threatened the people's quality of life and restricted the export of my country's agricultural products. The rampant occurrence of soil-borne diseases and the potential pollution of che...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12P1/04C12R1/465
Inventor 刘西莉卢晓红袁善奎李健强朱春雨刘鹏飞卢志军王红梅韩平黄中乔
Owner CHINA AGRI UNIV
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