A plurality of indolocarbazole alkaloids having strong insect disinfestation activities, and production strain thereof
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A technology of indolecarbazole and insecticidal activity, which is applied in the field of several indolecarbazole alkaloids with strong insecticidal activity and the production strains, achieving the effects of stable characteristics, low production cost and good development prospects
Inactive Publication Date: 2015-05-06
王林
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[0003]Because the impact of pests on cultivated plants is more significant than that of diseases, the market demand for pesticides is stronger, but in the existing research and development of pesticides, strong insecticide leads The probability of compounds is far less than that of lead compounds with antibacterial activity, that is, compounds with strong insecticidal activity are difficult to obtain in nature, and there is an urgent need to develop new resources. The research on terrestrial microorganisms is earlier, and the probability of discovering new agricultural microbial resources is significantly reduced. Marine The special habitat determines that the metabolic type of microorganisms is different from that of terrestrial microorganisms, and the probability of discovering new pesticide compounds will be greatly increased. Marine microorganisms have attracted much attention as a new resource for discovering new pesticide compounds
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Embodiment 1
[0015] The indolecarbazole alkaloid compound producing strain CQT14-24 is a marine habitat Streptomyces nitrosporeus, the strain is artificially cultivated, the fermented product is obtained and extracted by conventional chemical methods, and four indolecarbazole alkaloids can be obtained and 1 anthraquinone compound, characterized in that it comprises the steps of:
[0016] 1) The bacterial strain CQT14-24 with its preservation number as CGMCC No.4607 was placed in the following solid medium for cultivation for 5 days, and then transferred to the fermentation broth of the same formula for fermentation and cultivation. The formulation of the medium was: soluble starch 15- 20g, 150-200ml of soybean juice, 100-150ml of sea mud juice, KNO 3 1.1-1.5g, K 2 HPO 4 0.4-0.8g, MgSO 4 0.5-0.8g, NaCl, 12-20g, KCl0.04-0.09g, MgCl 2 0.23-0.50g, FeSO4 7H 2 O0.01-0.03g, multivitamin 1mL / L, water 650-750ml, pH7-7.5, do not add agar as culture medium, add agar 16-19g as solid medium, 25℃-2...
Embodiment 2
[0035] Feeding Pieris rapae, Brevicoryne brassicae, Hyphantria cunea, Plutella xyllostella, Mythimna separata, and Helicoverpa armigera to 2-3 instars by conventional methods First, the above five compounds were diluted to 20 μg / ml with 1% DMSO solution, cabbage and corn leaves were beaten into leaf butterflies, soaked in the test drug solution for 5 seconds, then taken out to dry, and put into the test samples starved for 5 hours respectively. The insects were reared at constant temperature in the insect culture room, and each treatment was repeated 3 times. The growth and development of the test insects were observed, weighed every 24 hours, and the weight of the test insects was recorded to calculate the inhibition rate of the growth and development of the test insects. After 96 hours, the mortality rate was corrected, and the pupation rate of the surviving test insects was observed with Pieris rapae as the target pest.
[0036] Insecticidal activity of 15 compounds in tabl...
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Abstract
The present invention discloses a plurality of indolocarbazole alkaloids having strong insect disinfestation activities, and a production strain of the indolocarbazole alkaloids. The indolocarbazole alkaloid compound production strain CQT14-24 is a marine Streptomyces nitrosporeus CQT14-24 strain, wherein the strain is subjected to artificial culture, and the obtained fermentation product is extracted by the conventional chemical method so as to obtain four indolocarbazole alkaloids and an anthraquinone compound. According to the present invention, the indolocarbazole alkaloids and the one anthraquinone compound provide strong killing effects on a variety of cultivated plant pests, particularly the pests having characteristics of spreading in agriculture and forestry production and serious harm and comprising Pieris rapae, Brevicoryne brassicae, Hyphantria cunea, Plutella xyllostella, Mythimna separate and Helicoverpa armigera, wherein the relative mortality rate achieves 81.9-100%, the polypide is seriously reduced, the growth inhibition rate is 100%, and the imago pupation rate is reduced by 70.4-86.7%; and the strain is subjected to acclimation through the artificial culture, and has characteristics of stable feature, easy culture, simple and feasible operation method, no environmental pollution, short period, cheap raw materials, low production cost, good development prospect, and easy industrialization achieving.
Description
technical field [0001] The invention belongs to the field of marine microorganisms, specifically several indolecarbazole alkaloids with strong insecticidal activity and the strains produced, which are preserved in the General Microbiology Center (CGMCC) of the China Microbiological Culture Collection Management Committee, and the address is Beijing No. 3 Beichen West Road, Chaoyang District, Institute of Microbiology, Chinese Academy of Sciences, date of deposit: February 25, 2011, deposit number CGMCC No.4607, classification name Streptomyces nitrosporus. Background technique [0002] my country is a large agricultural country, and the export of agricultural products occupies an important proportion in the national economy. In recent years, the international community has paid more and more attention to the problem of pesticide residues. The direct and indirect losses caused by the export of China's agricultural products amounted to more than billions. At the same time, th...
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