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Preparation method for dichloro substituted II-type halogenated polyketone compound and antibacterial activity application

A technology of polyketide compounds and chlorine substitution, which is applied in the field of microorganisms, can solve the problems of low yield, defects in preparation methods, and small yield, and achieve the effect of accelerating metabolism and improving fecundity

Active Publication Date: 2018-04-20
ZUNYI MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0007] However, due to the defect of the preparation method, the yield obtained is relatively small, and the yield is also low. At the same time, because the yield is very small (about 7mg for each compound), only the drug-resistant Staphylococcus aureus activity has been done.

Method used

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  • Preparation method for dichloro substituted II-type halogenated polyketone compound and antibacterial activity application
  • Preparation method for dichloro substituted II-type halogenated polyketone compound and antibacterial activity application
  • Preparation method for dichloro substituted II-type halogenated polyketone compound and antibacterial activity application

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Embodiment Construction

[0029] The present invention will be described in further detail below by means of specific embodiments:

[0030] Preparation method of dichloro-substituted type II halogenated polyketides

[0031] Step 1, activation of Streptomyces sp.FJS31-2 bacterial strain

[0032] Take out the strains preserved on the glycerol slant from the -80°C refrigerator, dig out 1 ring of spores of the strain Streptomyces sp. FJS31-2 with a sterile inoculation loop, and inoculate the 11cm-diameter basal medium plate with a cross-hatch, and let it stand at 28°C Cultivate for 3 days, pick a single colony and subculture to the third generation to scale up the culture.

[0033] Step 2, fermentation and cultivation of fermentation products

[0034] Streptomyces sp.FJS31-2 strains were fermented on improved GYD solid medium, and 150mL of fermentation medium was packed in a 500mL Erlenmeyer flask, and agar powder was added to each bottle according to the mass of agar powder and 1.8% of the volume of the...

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Abstract

The invention discloses a preparation method for a dichloro substituted II-type halogenated polyketone compound zunyimycin A, wherein the compound is derived from a secondary metabolite of a streptomyces sp.FJS31-2 strain (with the strain preservation number of CGMCC4.7321), and finally, a structure is identified and determined after strain activation, expansion culture and isolation. The compoundcan be used in treatment of diseases infected by gram-positive bacteria such as staphylococcus aureus (MRSA) and drug-resistant bacteria thereof, staphylococcus epidermidis, bacillus subtilis and thelike, is used in treatment of diseases infected by gram-negative bacteria such as super-spectrum beta-lactamase escherichia coli (ESBL), proteusbacillus vulgaris, bacterium burgeri and the like, andis used for treatment of diseases infected by fungi, such as candida albicans.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a preparation method and application of antibacterial activity of a dichloro-substituted halogenated type II polyketide compound, in particular to a preparation method and antibacterial activity of a dichloro-substituted halogenated type II polyketide compound zunyimycin A active application. Background technique [0002] Natural products derived from Streptomyces often have good biological activity and drug potential, and are an important source of antibiotics. However, with the abuse of antibiotics, the emergence of many "super bacteria" has brought huge challenges to the clinical treatment of diseases such as pathogenic bacterial infections, and some super bacteria have no cure. In view of the current increasingly severe antibacterial situation, increasing the research and development of antibiotics, exploring and developing new antibiotics has become a top priority, a...

Claims

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Application Information

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IPC IPC(8): C12P17/18A61K31/357A61P31/04A61P31/10C12R1/465
CPCA61K31/357C12P17/181Y02A50/30
Inventor 钱声艳杨彩玲刘建国王倩王苗王方远
Owner ZUNYI MEDICAL UNIVERSITY
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