Feed additive for improving reproductive capacity of boars by using 25-OH-VD3 and preparation method of feed additive

Abstract

The invention relates to the technical field of feed additives, and discloses a feed additive for improving the reproductive capacity of boars by using 25-OH-VD3 and a preparation method of the feed additive. The liquid composition agent comprises a plurality of composite vitamins, glycine, arginine, threonine, asparaginic acid, methionine, menadione sodium bisulfite, carnitine hydrochloride, glutamine, a liquid stabilizer sorbitol and purified water; the solid combination agent comprises 25-OH-VD3, vitamin A, vitamin E, arginine, glycine and an auxiliary material glucose. Starting from the overall thought of health, metabolism and the like, the problems of weak stress resistance, physique degeneration, reproductive capacity reduction and the like of breeding boars in modern pig raising are solved, the feed additive prepared by taking independently researched and developed high-quality 25-OH-VD3 as a main active substance achieves the comprehensive efficacy of resisting oxidation, enhancing immunity and improving reproductive capacity in modern pig raising, and the feed additive is suitable for large-scale production. Therefore, the reproductive capacity of the boars is improved.

Description

Technical Field

[0001] The invention relates to the technical field of feed additives, in particular to a feed additive utilizing 25-OH-VD3 to improve the reproductive capacity of boars and a preparation method thereof. Background Art

[0002] With the continuous and widespread application of artificial insemination technology for pigs in my country in recent years, the utilization rate of excellent breeding boars has been greatly improved, and the number of breeding boars raised has been greatly reduced. Especially with the widespread application of artificial insemination technology in intensive large-scale pig production, the semen quality of breeding boars has increasingly directly affected the development benefits of pig farms. Therefore, improving the semen quality of breeding boars has become an urgent problem to be solved in the actual production of pig farms, and it is also of great significance to the promotion of modern pig farming.

[0003] 25-OH-VD3 (25-OH-VD3) is vitamin D3 activated by the liver in animals. Vitamin D can achieve all-round regulatory effects on animal metabolism, strong bones, strong reproduction, stress resistance and improving the immune system. Especially in recent years, intensive large-scale pig farming has been accompanied by strict artificial insemination, and the burden of boar breeding has continued to increase, highlighting many reproductive disorders. It is necessary to solve the problem through the strengthening of abnormal nutrition and immune measures. Liquid feed is a new type of feed form that has gradually emerged in recent years. Liquid feed has incomparable advantages over dry feed in terms of increasing pig feed intake, feed conversion rate and growth performance.

[0004] The invention patent "An additive for improving the quality of boar semen and its application" (CN 113197264A), the additive contains 50-100 parts of proanthocyanidins, 100-150 parts of curcumin, 100-200 parts of lauric acid, 10-50 parts of vitamin E, 20-50 parts of oregano oil, 10-50 parts of thymol, 50-100 parts of sodium butyrate, 50-200 parts of Chinese herbal medicine extract, and 100-500 parts of carrier; the invention patent "Application of calcium hydroxymethionine and 25-hydroxyvitamin D3 in improving pig growth performance and reproductive performance (CN107183378A), calcium hydroxymethionine and 25-OH-VD3 are used in combination in feed at the same time, and calcium hydroxymethionine and 25-OH-VD3 produce a synergistic effect, which can significantly improve the weight gain of suckling piglets and improve the quality of boar semen.

[0005] At present, the existing technology usually improves the fertility of boars by providing trace nutrients, but lacks the joint reinforcement of the health and fertility of boars, which can easily affect the fertility of boars; therefore, it does not meet the existing needs. In this regard, we propose a feed additive that uses 25-OH-VD3 to improve the fertility of boars and a preparation method thereof. Summary of the invention

[0006] The present invention provides a feed additive for improving boar fertility by utilizing 25-OH-VD3 and a preparation method thereof, which has the beneficial effect of improving boar fertility by jointly strengthening the health and fertility of boars, and solves the problem that the prior art mentioned in the above background technology usually improves boar fertility by providing trace nutrients, but lacks joint strengthening of the health and fertility of boars, thus easily affecting the fertility of boars.

[0007] The present invention provides the following technical scheme: a feed additive for improving the fertility of boars by using 25-OH-VD3, the additive comprising a liquid composite agent, the liquid composite agent comprising the following components by weight: 2-4.5 parts of complex vitamins; 0.5-2 parts of glycine; 0.5-2 parts of arginine; 0.1-1.5 parts of threonine; 0.6-2.0 parts of aspartic acid; 0.3-2.2 parts of methionine; 0.01-0.10 parts of sodium bisulfite menadione; 0.1-1.2 parts of carnitine hydrochloride; 0.5-2.0 parts of glutamine; 6.0-20 parts of liquid stabilizer sorbitol; 60-90 parts of purified water, a total of 100 parts.

[0008] As an optional scheme for the feed additive of improving boar fertility by using 25-OH-VD3 described in the present invention, the compound vitamin 2-4.5 parts comprises 1.5-3 parts of nicotinamide with a content of 99.0%; 0.1-0.4 parts of thiamine nitrate with a content of 98.0%; 0.1-0.5 parts of riboflavin with a content of ≥98.0%; 0.1-0.5 parts of pyridoxine hydrochloride with a content of ≥98.0%; 0.001-0.004 parts of cyanocobalamin with a content of ≥99.0%; and 0.01-0.04 parts of biotin with a content of ≥98.0%.

[0009] As an optional scheme of feed additive for improving boar fertility by using 25-OH-VD3 according to the present invention, wherein: the liquid composition has a glycine content of ≥98.5%; an arginine content of ≥98.5%; a threonine content of ≥98.5%; an aspartic acid content of ≥99.0%; a methionine content of ≥98.5%; and a sodium bisulfite menadione content of ≥50.0%;

[0010] The liquid combination is prepared by heating 1000 parts of purified water to 20°C-50°C; firstly, adding 6.0-20 parts of antioxidants and 1.9-5.4 parts of water-soluble vitamins in sequence, stirring while adding the materials until they are completely dissolved; then, adding 2.5-11.7 parts of amino acids, stirring while adding the materials; finally, stirring at 20°C-50°C for 10-30 minutes, and the liquid becomes red or orange.

[0011] As an optional scheme for a feed additive for improving boar fertility by using 25-OH-VD3 according to the present invention, the additive also includes a solid composition, which includes the following components by weight: 5-12 parts of 25-OH-VD3 with a content of 0.05%; 1.5-2.5 parts of vitamin A with a content of 500,000 IU / g; 3-5 parts of vitamin E with a content of 50%; 0.1-0.5 parts of arginine with a content of 98.5%; 0.1-0.5 parts of glycine with a content of 98.5%; and 80-90 parts of glucose as an auxiliary material, totaling 100 parts.

[0012] As an optional scheme for a feed additive for improving boar fertility by using 25-OH-VD3 described in the present invention, wherein: the 25-OH-VD3 5-12 parts is a 25-OH-VD3 diluent with a specification of 0.05% created by the unit of the present invention, which is prepared by dissolving 25-hydroxy-7-dehydrocholesterol in a mixed solvent system comprising a polar solvent and a non-polar solvent, wherein the volume ratio of the polar solvent to the non-polar solvent is 9-4:1, adding an antioxidant, and reacting under light; monitoring the reaction process by high performance liquid chromatography, controlling the raw material conversion rate to 32-39%, separating and recovering 25-hydroxy-7-dechlorocholesterol after the reaction, and then heating the remaining reactant after separating 25-hydroxy-7-dehydrocholesterol for isomerization, and then freezing for recrystallization to obtain 25-OH-VD3 crystals with a purity of ≥94%.

[0013] As an optional feed additive scheme for improving boar fertility by using 25-OH-VD3 as described in the present invention, wherein: under normal temperature environment, 1 kg of the solid composition is added per ton of feed, and 20 mL of the liquid composition is added per boar per day; in high temperature seasons, 1.5 kg of the solid composition is added per ton of feed, and 40 mL of the liquid composition is added per boar per day.

[0014] A method for preparing a feed additive for improving boar fertility by using 25-OH-VD3, wherein: the liquid combination is prepared by mixing corresponding components, the solid combination is prepared by mixing corresponding components, and the additive is used in combination with the liquid combination and the solid combination.

[0015] The present invention has the following beneficial effects:

[0016] 1. The feed additive and preparation method for improving boar fertility by using 25-OH-VD3 use the 25-OH-VD3 product created and synthesized by independent domestic leading technology as the main effect product. Starting from the overall idea of ​​improving boar health and metabolism, while strengthening the main effect of 25-OH-VD3, it selects unique other vitamins, trace elements and targeted active substances to construct a companion-type optimized combination, which enhances the extensive functional effectiveness of 25-OH-VD3 and works together to solve the production problems of modern pig farming, such as the decline in semen quality and shortened utilization cycle of boars, thereby improving boar fertility.

[0017] 2. The feed additive and preparation method for improving the reproductive capacity of boars using 25-OH-VD3 use innovative 25-OH-VD3 as the main active substance, expand the traditional use of vitamin D as the "golden partner" of calcium and phosphorus metabolism, and apply the research results in recent years that vitamin D can achieve the regulation of more than 2,000 genes in almost all tissues and organs of the animal body, thereby improving the comprehensive functions of 25-OH-VD3 in modern pig farming, such as anti-oxidation, strong immunity and improving reproductive capacity.

[0018] 3. The feed additive and preparation method for improving the reproductive capacity of boars using 25-OH-VD3 adopt a companion liquid combination agent and a solid combination agent for use in combination. Compared with normal feed, different feeding methods are adopted according to the characteristics of different active substances. It has incomparable advantages in reducing costs and increasing efficiency, such as improving the feed conversion rate and growth performance of breeding boars, enhancing reproductive performance, and reducing the waste of trace nutrients. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 The present invention is a flow chart for preparing the liquid composition. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] Embodiment 1: This embodiment aims to promote the solution of the existing technology, which usually improves the fertility of boars by providing trace nutrition, but lacks the joint strengthening of the health and fertility of the boars, which easily affects the fertility of the boars. Figure 1A feed additive for improving the reproductive capacity of boars by using 25-OH-VD3, including a liquid composition and a solid composition. The liquid composition includes the following components by weight: 2.5-4.0 parts of complex vitamins; 0.8-2 parts of glycine; 0.6-2 parts of arginine; 0.1-1.5 parts of threonine; 0.7-2.0 parts of aspartic acid; 0.8-2.0 parts of methionine; 0.05-0.10 parts of sodium bisulfite menadione; 0.3-1.2 parts of carnitine hydrochloride; 0.8-2.0 parts of glutamine; 8.0-20 parts of liquid stabilizer sorbitol; 60-85 parts of purified water, totaling 100 parts.

[0022] 2.0-4.0 parts of complex vitamins, including 1.7-3 parts of nicotinamide with a content of 99.0%; 0.1-0.3 parts of thiamine nitrate with a content of 98.0%; 0.1-0.4 parts of riboflavin with a content of ≥98.0%; 0.1-0.4 parts of pyridoxine hydrochloride with a content of ≥98.0%; 0.001-0.004 parts of cyanocobalamin with a content of ≥99.0%; and 0.01-0.03 parts of biotin with a content of ≥98.0%.

[0023] The liquid combination preparation has a glycine content of ≥98.5%, an arginine content of ≥98.5%, a threonine content of ≥98.5%, an aspartic acid content of ≥99.0%, a methionine content of ≥98.5%, and a sodium bisulfite menadione content of ≥50.0%.

[0024] The preparation of the liquid combination is based on parts by weight: heat 1000 parts of purified water to 20°C-50°C; first add 6.0-20 parts of antioxidants and 1.9-5.4 parts of water-soluble vitamins in sequence, stirring while adding until completely dissolved; then add 2.5-11.7 parts of amino acids, stirring while adding; finally stir at 20°C-50°C for 10-30 minutes, and it will become a red or orange liquid.

[0025] The solid composition comprises the following components by weight: 35-10 parts of 25-OH-VD with a content of 0.05%; 2.0-2.4 parts of vitamin A with a content of 500,000 IU / g; 4-5 parts of vitamin E with a content of 50%; 0.1-0.5 parts of arginine with a content of 98.5%; 0.1-0.5 parts of glycine with a content of 98.5%; and 80-90 parts of glucose as an auxiliary material, totaling 100 parts.

[0026] Furthermore, 5-10 parts of 25-OH-VD3 is a 0.05% 25-OH-VD3 diluent created by the present invention, and the purity of the 25-OH-VD3 crystals obtained by chemical synthesis technology is ≥94%.

[0027] A method for preparing a feed additive for improving the reproductive capacity of boars by using 25-OH-VD3. The liquid composite agent is prepared by mixing corresponding components, and the solid composite agent is prepared by mixing corresponding components. The additive is a liquid composite agent and a solid composite agent used in combination. Under normal temperature, 1 kg of the solid composite agent is added per ton of feed, and 20 mL of the liquid composite agent is added per boar every day; in high temperature seasons, 1.5 kg of the solid composite agent is added per ton of feed, and 40 mL of the liquid composite agent is added per boar every day.

[0028] In this embodiment: the 25-OH-VD3 product created and synthesized by independent domestic leading technology is used as the main effect product. Starting from the overall idea of ​​improving boar health and metabolism, while strengthening the main effect of 25-OH-VD3, unique other vitamins, trace elements and targeted active substances are preferably selected to construct a companion-type optimized combination, thereby enhancing the wide range of functional benefits of 25-OH-VD3, and working together to solve the production problems of modern pig farming such as the decline in semen quality and shortened utilization cycle of boars, thereby improving the fertility of boars, and solving the existing technology as much as possible. The fertility of boars is usually improved by providing trace nutrients, but there is a lack of joint strengthening of the health and fertility of boars, which easily affects the fertility of boars.

[0029] Embodiment 2: This embodiment is an optional solution of Embodiment 1. For details, please refer to Figure 1 The liquid composition comprises the following components by weight: 3-4.0 parts of complex vitamins; 1-2 parts of glycine; 1-2 parts of arginine; 1-1.5 parts of threonine; 1-2.0 parts of aspartic acid; 1-2.0 parts of methionine; 0.08-0.10 parts of sodium bisulfite menadione; 0.5-1.2 parts of carnitine hydrochloride; 1-2 parts of glutamine; 10.0-20 parts of liquid stabilizer sorbitol; 60-85 parts of purified water, totaling 100 parts.

[0030] The contents of the multivitamins and other active substances are the same as those in Example 1, and the preparation of the liquid combination is the same as that in Example 1.

[0031] The solid composition comprises the following components by weight: 6-11 parts of 25-OH-VD3 with a content of 0.05%; 1.8-2.4 parts of vitamin A with a content of 500,000 IU / g; 4-5 parts of vitamin E with a content of 50%; 6-10 parts of 25-OH-VD3 with a content of 0.05%; 0.1-0.5 parts of arginine with a content of 98.5%; 0.1-0.5 parts of glycine with a content of 98.5%; and 80-88 parts of glucose as an auxiliary material, totaling 100 parts.

[0032] 25-OH-VD3 5-10 parts are used as in Example 1.

[0033] Embodiment 3: This embodiment is an optional solution of Embodiment 1. For details, please refer to Figure 1 The liquid composition comprises the following components by weight: 3.5-4.2 parts of complex vitamins; 1.5-2 parts of glycine; 1-2 parts of arginine; 1.2-2.0 parts of threonine; 1.5-2.0 parts of aspartic acid; 1-2.0 parts of methionine; 0.08-0.10 parts of sodium bisulfite menadione; 0.5-1.2 parts of carnitine hydrochloride; 1-2 parts of glutamine; 10.0-20 parts of liquid stabilizer sorbitol; 60-80 parts of purified water, totaling 100 parts.

[0034] The contents of the multivitamins and other active substances are the same as those in Example 1, and the preparation of the liquid combination is the same as that in Example 1.

[0035] The solid composition comprises the following components by weight: 8-12 parts of 25-OH-VD3 with a content of 0.05%; 2-2.4 parts of vitamin A with a content of 500,000 IU / g; 4-6 parts of vitamin E with a content of 50%; 6-10 parts of 25-OH-VD3 with a content of 0.05%; 0.1-0.5 parts of arginine with a content of 98.5%; 0.1-0.5 parts of glycine with a content of 98.5%; and 80-88 parts of glucose as an auxiliary material, totaling 100 parts.

[0036] 25-OH-VD3 5-10 parts are used as in Example 1.

[0037] 1. Experimental Example 1: Effects of a feed additive utilizing 25-OH-VD3 to improve boar fertility on the fertility and antioxidant properties of boars in normal temperature seasons.

[0038] 1) Materials and methods

[0039] 1.1 Experimental Materials: The feed additives used in the feeding experiment were prepared according to the feed additive preparation methods in Examples 1, 2 and 3 of the present invention.

[0040] 1.2 Experimental animals and diets

[0041] In the normal temperature season (the experimental period was March 6-May 7, 2023), 40 Duroc boars aged 16-20 months with similar weight and semen quality before the experiment were selected and randomly divided into 4 groups, 1 control group and 3 experimental groups, 10 pigs in each group, and 1 pig in each repetition. The experimental period was 9 weeks. The control group was fed a basic diet, and the three experimental groups (experimental group 1, experimental group 2 and experimental group 3) added the additives of the solid combination agent + liquid combination agent of Example 1, Example 2 and Example 3 to the basic diet respectively.

[0042] The basal diet was a corn-soybean meal type diet, and the feed was formulated with reference to the nutritional levels recommended by NRC. See Table 1 for details, and Table 2 for the experimental design.

[0043] Table 1 Basic diet and nutritional level

[0044]

[0045]

[0046] Table 2 Dietary feeding design for experimental groups

[0047]

[0048] 1.3 Index determination

[0049] (1) Semen quality analysis

[0050] At the end of the 3rd, 6th and 10th week of the experimental period, boar semen was collected by manual semen collection to test semen quality. The semen collection period was 8:00-10:00 in the morning. Computer-assisted semen analysis system (CASA) was used to detect semen quality-related parameters (semen volume, sperm density, sperm motility, deformity rate and sperm forward motility) for analysis. The specific operations are as follows: 1. Preheat the CASA detection system and preheat the constant temperature heating table at 37℃; 2. Use a pipette to draw 10μL of semen sample and inject it into the sperm detection plate cavity; 3. Place the sperm detection plate on the heating table and let it stand at 37℃ for 3min; 4. Transfer the sperm detection plate to the detection system stage, select the initial field of view after injection, and click to start the test. The system automatically selects 5 different fields of view to collect and analyze the sperm status and movement in the field of view, and record various index data.

[0051] The three measurement results of each sample in the experimental period were combined into one sample data for statistical analysis of differences.

[0052] (2) Analysis of semen antioxidant indicators

[0053] On the day when the experiment ended, 10 mL of semen was retained in a glass centrifuge tube. After static precipitation, 2 mL of the upper layer of seminal plasma was taken and stored at -15°C for testing. The total antioxidant capacity (T-AOC) content in seminal plasma was determined by colorimetry, the malondialdehyde (MDA) content was determined by thiobarbituric acid method, the superoxide dismutase (SOD) activity was determined by xanthine oxidase assay, and the glutathione peroxidase (GSH-Px) activity was determined by colorimetry. The above indicators were determined by the kit of Nanjing Jiancheng Bioengineering Research Institute, and the specific operations were carried out according to the instructions.

[0054] The statistical data were analyzed using conventional difference test methods.

[0055] 2) Results and analysis

[0056] 2.1 Effect of the additive of the present invention on the semen quality of boars

[0057] Table 3 Effect of the additive of the present invention on the semen quality of boars

[0058]

[0059] As shown in Table 3, the additives of the high-efficiency companion type liquid combination agent and solid combination agent of the present invention, and the micronutrient functional combination agent can significantly increase the semen collection amount of boars in normal temperature season, increase the density of semen, improve sperm motility, reduce the sperm deformity rate, and increase the proportion of sperm forward movement. It has a significant effect on enhancing the reproductive capacity of boars.

[0060] 2.2. Effect of the additive of the present invention on the antioxidant function of boar seminal plasma

[0061] Table 4 Effect of the additive of the present invention on the antioxidant function of boar seminal plasma

[0062]

[0063] As can be seen from Table 4, the additive of the present invention can significantly improve the antioxidant function of boars in normal temperature seasons and improve the levels of total antioxidant capacity, superoxide dismutase, glutathione peroxidase, etc. in seminal plasma.

[0064] 2. Experimental Example 2: Effects of the additive of the present invention on the reproductive capacity and antioxidant performance of boars in hot seasons.

[0065] 1). Materials and methods

[0066] 1.1 Experimental Materials: The feed additives used in the feeding experiment were prepared according to the feed additive preparation methods in Examples 1, 2 and 3 of the present invention.

[0067] 1.2 Experimental animals and diets

[0068] In the high temperature season (the experimental period was July 2-August 26, 2023), 32 Duroc boars of about 18 months of age and similar weight and semen quality before the experiment were selected and randomly divided into 4 groups, 1 control group and 3 experimental groups, 8 pigs in each group, and 1 pig in each repetition. The experimental period was 8 weeks (July-August). The control group was fed a basic diet, and the three experimental groups (experimental group 1, experimental group 2 and experimental group 3) added the additives of the solid combination agent + liquid combination agent of Example 1, Example 2 and Example 3 to the basic diet respectively.

[0069] The basic diet was a corn-soybean meal type diet, and the feed was formulated with reference to the nutritional level recommended by NRC nutrient requirements. For details, see Table 1 of Experimental Example 1, and for details of the experimental design, see Table 5.

[0070] Table 5. Daily feeding design of experimental groups during high temperature experimental period

[0071]

[0072] 1.3 Index determination

[0073] (1) Semen quality analysis

[0074] At the end of the 2nd, 4th, 6th and 8th week of the experimental period, boar semen was collected by hand to test the semen quality. The semen collection period was 8:00-10:00 am.

[0075] (2) Analysis of semen antioxidant indicators

[0076] Same as Experimental Example 1;

[0077] 2) Results and analysis

[0078] 2.1 Effect of the additive of the present invention on the semen quality of boars

[0079] Table 6 Effect of the additive of the present invention on the semen quality of boars in the high temperature period

[0080]

[0081] As shown in Table 6, the additive of the present invention significantly increases the amount of semen collected from boars in the hot season, increases the density of semen, improves sperm motility, reduces the sperm deformity rate, and increases the proportion of sperm forward movement. It has a significant application effect on enhancing the reproductive capacity of boars in the hot season.

[0082] 2.2. Effect of the additive of the present invention on the antioxidant function of boar seminal plasma

[0083] Table 7 Effect of the additives of the present invention on the antioxidant function of boar semen during high temperature period

[0084]

[0085]

[0086] As can be seen from Table 7, the additive of the present invention can significantly improve the antioxidant function of boars in hot seasons, increase the total antioxidant capacity of seminal plasma, the levels of superoxide dismutase, glutathione peroxidase, etc., and reduce the malondialdehyde content of seminal plasma.

[0087] 3. Conclusions of the experiment and production application of the present invention

[0088] The present invention uses high-quality 25-OH-VD3 independently developed by the inventor as the main active substance. The feed additive developed using 25-OH-VD3 to improve boar fertility has a significant promoting effect on the fertility and antioxidant properties of boars in both normal and high temperature seasons, especially in high temperature seasons.

[0089] The above is only one of the preferred embodiments of the present invention. For technicians in the field of pig farming, based on the technical principles of the present invention and combined with the specific reality of their own enterprises, especially the physical condition and health of breeding sows, they can make several further optimizations and improvements to improve the technical effects of the practical application of the present invention.

[0090] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0091] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the technical principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A feed additive for improving boar fertility by using 25-OH-VD3, comprising a liquid composition and a solid composition, characterized in that: The liquid composition comprises a variety of complex vitamins, glycine, arginine, threonine, aspartic acid, methionine, sodium menadione bisulfite, carnitine hydrochloride, glutamine, liquid stabilizer sorbitol, and purified water; The solid composition comprises 25-OH-VD3, vitamin A, vitamin E, arginine, glycine and auxiliary material glucose.

2. A feed additive for improving boar fertility by using 25-OH-VD3 according to claim 1, characterized in that: The liquid composition comprises the following components by weight: 2-4.5 parts of complex vitamins; 0.5-2 parts of glycine; 0.5-2 parts of arginine; 0.1-1.5 parts of threonine; 0.6-2.0 parts of aspartic acid; and 0.3-2.2 parts of methionine. 0.01-0.10 parts of sodium bisulfite menadione; 0.1-1.2 parts of carnitine hydrochloride; 0.5-2.0 parts of glutamine; 6.0-20 parts of liquid stabilizer sorbitol; 60-90 parts of purified water, totaling 100 parts.

3. A feed additive for improving boar fertility by using 25-OH-VD3 according to claim 2, characterized in that: The said multivitamin 2-4.5 parts are 1.5-3 parts of nicotinamide with a content of 99.0%; 0.1-0.4 parts of thiamine nitrate with a content of 98.0%; 0.1-0.5 parts of riboflavin with a content of ≥98.0%; 0.1-0.5 parts of pyridoxine hydrochloride with a content of ≥98.0%; 0.001-0.004 parts of cyanocobalamin with a content of ≥99.0%; 0.01-0.04 parts of biotin with a content of ≥98.0%.

4. A feed additive for improving boar fertility by using 25-OH-VD3 according to claim 2, characterized in that: The liquid composition is prepared by weight: 1000 parts of purified water are heated to 20°C-50°C; first, 6.0-20 parts of antioxidants and 1.9-5.4 parts of water-soluble vitamins are added in sequence, and stirred while adding the materials until they are completely dissolved; then, 2.5-11.7 parts of amino acids are added, and stirred while adding the materials; finally, the mixture is stirred at 20°C-50°C for 10-30 minutes until it becomes a red or orange liquid.

5. A feed additive for improving boar fertility by using 25-OH-VD3 according to claim 1, characterized in that: The solid composition comprises the following components by weight: 5-12 parts of 25-OH-VD3 with a content of 0.05%; 1.5-2.5 parts of vitamin A with a content of 500,000 IU / g; 3-5 parts of vitamin E with a content of 50%; 0.1-0.5 parts of arginine with a content of 98.5%; 0.1-0.5 parts of glycine with a content of 98.5%; and 80-90 parts of auxiliary material glucose, totaling 100 parts.

6. A feed additive for improving boar fertility by using 25-OH-VD3 according to claim 1, characterized in that: Under normal temperature conditions, 1 kg of the solid composition is added per ton of feed, and 20 mL of the liquid composition is added per boar per day; in high temperature seasons, 1.5 kg of the solid composition is added per ton of feed, and 40 mL of the liquid composition is added per boar per day.

7. A method for preparing a feed additive for improving boar fertility by using 25-OH-VD3, used for the feed additive for improving boar fertility by using 25-OH-VD3 as claimed in claim 1, characterized in that: The liquid composition is prepared by mixing its corresponding components, the solid composition is prepared by mixing its corresponding components, and the additive is used in combination with the liquid composition and the solid composition.

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