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Plant pathogenic fungi antagonistic bacteria strain and its use in control of plant diseases

A plant disease and disease technology, applied in applications, plant growth regulators, botanical equipment and methods, etc., can solve problems such as production constraints, long breeding cycles, and high manpower and funds.

Inactive Publication Date: 2008-08-27
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of chemical agents to prevent and control the disease is particularly difficult, because while killing pathogenic bacteria in the soil, it also inhibits the growth of beneficial bacteria, destroys the micro-ecology of the soil, and at the same time causes great adverse effects on fruits and the environment
The use of disease-resistant varieties is greatly restricted in production due to the long breeding cycle, the need for manpower and funds
There have been many reports on the use of endophytic bacteria to control plant diseases, but there has been no report on the use of endophytic bacteria to control banana wilt at home and abroad.

Method used

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  • Plant pathogenic fungi antagonistic bacteria strain and its use in control of plant diseases
  • Plant pathogenic fungi antagonistic bacteria strain and its use in control of plant diseases
  • Plant pathogenic fungi antagonistic bacteria strain and its use in control of plant diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1, screening and identification of phytopathogenic fungus antagonistic bacteria

[0019] 1. Screening of antagonistic bacteria of plant pathogenic fungi

[0020] The bacterial strain of the present invention is isolated from the inside of a healthy banana plant in Xiqing Farm, Danzhou, Hainan. The specific method is: take 1 g of healthy banana plant tissue, after surface disinfection, put it into a sterilized mortar with 10 mL of sterile water, Add a little sterilized quartz sand to grind evenly, and let it stand for 15 minutes. Take 1mL and dilute to 10 -3 、10 -4 、10 -5 Concentration gradient, take 0.1mL from each concentration gradient suspension, and use a sterile applicator to spread it on NA medium (each 1000mL contains 5g of peptone, 1g of yeast extract, 3g of beef extract, 15g of agar powder, 10g of glucose, pH 7.0 ) plate, and the sterile water that washed the sample last time was used as the control. Inverted culture at 28°C, observed continuous...

Embodiment 2

[0049] Embodiment 2, Bacillus subtilis (Bacillus subtilis) BEB2 CGMCC No.2243 antibacterial spectrum determination

[0050] Take the Bacillus subtilis (Bacillus subtilis) BEB2 CGMCC No.2243 culture solution (28°C, shake Bed cultured for 24 hours, OD 600 The value is about 2.5) and 2mL is poured into 15mL PDA medium cooled to about 45°C, shaken gently to make the Bacillus subtilis (Bacillus subtilis) BEB2 bacterial solution and the medium fully mixed, and poured into a 9cm diameter Petri dish, Cooling is made into flat board, and the test pathogenic bacteria bacterium block (purchased from China Agricultural Microorganism Culture Collection Management Center, preservation number ACCCNo.31272) and banana anthracnose bacteria (purchased from China Agricultural Microbiology Culture Collection Management Center, preservation number ACCCNo.31247), rice blast fungus (purchased from China Agricultural Microorganism Culture Collection Management Center, preservation number ACCCNo.3032...

Embodiment 3

[0053] Embodiment 3, endophytic determination of Bacillus subtilis (Bacillus subtilis) BEB2 CGMCC No.2243

[0054] 1. Acquisition of resistance to chloramphenicol and streptomycin in Bacillus subtilis BEB2 CGMCC No.2243

[0055] Inoculate Bacillus subtilis (Bacillus subtilis) BEB2 CGMCC No.2243 into NB culture solution containing 10 μg / mL chloramphenicol and 10 μg / mL streptomycin, shake the bacteria for about 48 hours until the bacteria liquid becomes cloudy, take a small amount of culture The bacterial solution was inoculated into the NB culture solution containing 20 μg / mL chloramphenicol and 20 μg / mL streptomycin, and the bacterial solution was shaken for about 48 hours until the bacterial solution became cloudy. According to this method, the concentrations of chloramphenicol and streptomycin were gradually increased for cultivation, and finally Bacillus subtilis (Bacillus subtilis) BEB2 CGMCC No. .2243 cells, according to the method in Example 1, confirm that the biologic...

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Abstract

The invention discloses a strain of plant pathogenic fungi antagonistic bacterium and the application of the plant pathogenic fungi antagonistic bacterium in preventing and curing plant diseases. The plant pathogenic fungi antagonistic bacterium is bacillus subtilis BEB2 CGMCC No.2243. The plant pathogenic fungi antagonistic bacterium bacillus subtilis BEB2 CGMCC No.2243 can prevent and cure the plant diseases which are caused by banana vascular wilt pathogen, colletotrichum musae, pyricularia grisea, corynespora leaf-fall disease of hevea rubber, bamboo fusarium axysporum and mango anthracnose pathogen, and the plant pathogenic fungi antagonistic bacterium bacillus subtilis BEB2 CGMCC No.2243 in particular can inter-grow with bananas, can survive in the bananas stably, and can prevent and cure banana panama diseases.

Description

technical field [0001] The invention relates to a plant pathogenic fungus antagonistic bacteria and its application in preventing and treating plant diseases. Background technique [0002] Plant endophytic bacteria refer to bacteria that can enter living plant tissues during their lifetime or at least during a certain period of their lifetime, and their existence does not significantly change the phenotypic characteristics and functions of plant tissues. In 1926, Perotti discovered the existence of endophytic bacteria in the root tissues of many healthy plants, and then the research in this area made great progress, and a variety of endophytic bacteria were found in the tissues of many plants. These endophytic bacteria use plant tissue as their habitat, and often promote growth, prevent diseases and insect pests, fix nitrogen, resist adversity (such as drought, etc.), and resist feeding by animals (insects, nematodes, herbivorous mammals, etc.) , anti-pathogenic fungi and b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/125
Inventor 黄贵修付业勤时涛刘先宝蔡吉苗
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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