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Antifungal streptomyces hygroscopicus and application thereof

A water-absorbing Streptomyces and antifungal technology is applied in the field of microorganisms to achieve the effect of promoting plant growth and good development and application prospects

Active Publication Date: 2022-01-21
HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, no one has studied the endophytic actinomycetes of medicinal plants in Wuzhishan Nature Reserve. This kind of unknown resources to be developed has great application potential in medicine and health, biological control and plant growth promotion, and provides a source for the prevention and control of banana wilt disease. new biological control resources

Method used

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  • Antifungal streptomyces hygroscopicus and application thereof
  • Antifungal streptomyces hygroscopicus and application thereof
  • Antifungal streptomyces hygroscopicus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Isolation identification and antibacterial activity screening of embodiment 1 bacterial strain

[0036] 1.1 Test materials

[0037] 1.1.1 Collection of medicinal plant samples

[0038] The medicinal plant specimens were collected from Wuzhishan Nature Reserve in Hainan Province in August 2016 (Table 1-1). Samples of 35 healthy plants, including roots, stems and leaves, were collected. Samples were stored in sterile plastic bags at 4°C.

[0039] Table 1-1 Medicinal plant samples and sampling locations

[0040]

[0041]

[0042] 1.1.2 Test medium

[0043] The test medium mainly includes separation medium (Table 1-2), morphology observation medium (Table 1-3) and physiological and biochemical characteristics medium (Table 1-4).

[0044] Table 1-2 Isolation medium and formula of actinomycetes

[0045]

[0046] Table 1-3 culture characteristics observation medium and its formula

[0047]

[0048] Table 1-4 Media required for observation of physiological an...

Embodiment 2

[0115] Antibacterial activity assay of embodiment 2 bacterial strain PA-4

[0116] 2.1 Test materials

[0117] Pathogens tested: Fusarium oxysporum f.sp. CubenseRace 4, Foc4, Colletotrichum acutatum, Colletotrichum fragariae, Colletotrichum gloeosporioides, Chestnut Cryphonectria parasitica, Fusarium oxysporum f.spcucumerinum, Fusarium oxysporum f.sp.Lycopersici, Fusarium oxysporum f.sp.Cubense race1, Foc1 ), Fusa Humgraminearum, Alternaria tenuissima, Ustilaginoidea oryzae, Pyricularia oryae Cav, Botrytis cinerea , Curvularia fallax, and Curvularia lunata were used for the determination of antibacterial spectrum. The pathogenic bacteria tested above are all strains preserved by the Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences.

[0118] Medium: PDA medium was used for the preparation of Foc4 cells; YE medium was used for activation of Streptomyces; the fermentation medium was optimized soybean meal medium (SLM), and soybea...

Embodiment 3

[0143] The control effect of embodiment 3 streptomyces PA-4 to banana wilt

[0144] 3.1 Test materials

[0145] Test crops: Brazil banana (Musa sp. AAA Cavendish subgroup cv. Brazil) is a susceptible variety, and nutrient cup tissue culture seedlings of the same size at the 3-4 leaf stage were selected for the test.

[0146] Preparation of pathogenic spore suspension: the tested pathogen is Fusarium oxysporum f.sp. Cubense Race 4 (GFP-Foc4) with green fluorescent protein marker, which was provided by the Chinese Academy of Tropical Agricultural Sciences for Environmental and Plant Protection. provided by the Institute. The freshly cultured GFP-Foc4 hyphae were picked and inoculated on PDA solid medium, and cultured at 28°C for 7 days. Scrape and wash the spores with sterile distilled water, filter with sterile filter paper, collect the suspension of pathogenic bacteria spores, count on a hemocytometer, and adjust to a concentration of 6.0×10 with sterile water. 5 cfu / mL, sp...

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Abstract

The invention provides streptomyces hygroscopicus, which is named streptomyces hygroscopicus PA-4 and is preserved in Guangdong Microbial Culture Collection Center on May 24, 2021, and the preservation number is GDMCC No.61679. The strain has stable and broad-spectrum bacteriostatic activity and has a relatively strong bacteriostatic effect on various common crop pathogenic fungi such as fusarium oxysporum, colletotrichum, cryptoscoparia, corynespora, alternaria, fusarium and the like, a fermentation crude extract of the strain can influence normal growth and development of Foc4 mycelia, spores and the like and has a poisoning or inhibiting effect on Foc4 germs. In addition, the streptomyces hygroscopicus disclosed by the invention can also inhibit pathogenic bacteria from infecting plant root systems and promote plant growth, expands a new field for preventing and treating various plant diseases such as fusarium wilt, promoting plant growth and the like, and has a good development and application prospect.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to an antifungal Streptomyces hygroscopicus and its application. Background technique [0002] Banana is a Musa plant of the Musaceae family, mainly distributed in the tropics and subtropics, and is one of the most important fruits in terms of production and trade in the world. The global annual output of bananas is about 148 million tons, and its main production areas are Asia and Central and South America. It is the second largest fruit crop in the world after citrus, and provides staple food for about 400 million people in the world. China is the fourth major producer of bananas in the world, with an output of 11.1698 million tons in 2017. Banana occupies an important position in the domestic market. It is not only a very popular fruit in our country, but also occupies an important position in export products. When the banana industry continued to develop and gradually a...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/28A01P3/00A01P21/00C12R1/55
CPCC12N1/20A01N63/28Y02E50/10
Inventor 云天艳臧小平井涛谢江辉王尉周登博张妙宜陈宇丰
Owner HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
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