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Bactericidal composition for preventing and treating tobacco corynespora leaf spot disease and application thereof

A technology of Corynespora leaf spot and composition, which is applied in the fields of application, fungicide, biocide, etc., can solve the problem of product registration of tobacco Corynesporium leaf spot without composition, and no public composition of Corynespora tobacco Leaf spot disease reports and other issues, to achieve the effect of slowing down the resistance of bacteria and reducing the dosage

Inactive Publication Date: 2022-05-20
ANHUI AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The applicant's preliminary research found that the fungicidal composition containing fludioxonil and prochloraz was disclosed in the prior art, but there was no relevant report on the use of the composition for tobacco Corynesporium leaf spot; and the Pesticide Information Network query, There is also no related product registration for the composition used for tobacco Corynesporium leaf spot

Method used

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  • Bactericidal composition for preventing and treating tobacco corynespora leaf spot disease and application thereof
  • Bactericidal composition for preventing and treating tobacco corynespora leaf spot disease and application thereof
  • Bactericidal composition for preventing and treating tobacco corynespora leaf spot disease and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Fungicide Screening

[0027] The indoor virulence of the tested fungicides to Corynebacterium is determined by the mycelial growth rate method, and the specific methods are as follows:

[0028] (1) Weigh 0.05g of the original drug of the bactericide into a 50mL centrifuge tube, and use a 10mL pipette to absorb 5mL of acetone (the solvent of different original drugs is different, the solvent of most original drugs is acetone, the solvent used to dissolve carbendazim hydrochloric acid) was added into the centrifuge tube to fully dissolve the original drug, and then diluted to 50mL with sterile water, and this solution A was 1000mg / L mother liquor;

[0029] (2) before adding the medicament to the culture medium, shake up the mother liquor, draw a certain amount of the mother liquor from the mother liquor according to the set plate concentration and add it to the PDA medium of 60 mL, and pour it into 3 culture dishes with a diameter of 90 mm on average after mixing. The pl...

Embodiment 2

[0040] Compounding agent screening

[0041] The method of Sun Yunpei was used to determine the combined virulence of different proportions of compounds against Corynebacterium:

[0042] The mother liquor B and C with a concentration of 1000 mg / L were prepared. After mixing 10 mL of mother liquor B and 1 mL of mother liquor C, the mother liquor with a B:C mass ratio of 10:1 was obtained. Similarly, 8:1, 6:1, and 4:1 were obtained. , 2:1, 1:2, 1:4, 1:6, 1:8, 1:10 ratio solutions, the solution concentration is 1000mg / L.

[0043] The experiment was repeated three times.

[0044] Theoretical EC 50 value and synergy coefficient SR, the calculation method is as follows:

[0045]

[0046] Note: a: Content of TC A in the formula; b: Content of TC B in the formula; A1: EC of TC A 50 ;B1: EC of technical drug B 50 .

[0047]

[0048] Note: SR>1.5 means synergistic effect;

[0049] 0.5≤SR≤1.5 is the additive effect;

[0050] SR<0.5 means antagonism.

[0051] The specific ex...

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PUM

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Abstract

The invention belongs to the field of pesticide bactericides, and particularly relates to a bactericidal composition for preventing and treating tobacco corynespora leaf spot and application of the bactericidal composition. Active components in the bactericidal composition consist of fludioxonil and prochloraz, and the mass ratio of fludioxonil to prochloraz is (1: 6)-(1: 10) and (1: 15). Or the active ingredients in the bactericidal composition are composed of epoxiconazole and prochloraz, and the mass ratio of epoxiconazole to prochloraz is 1: 1, 1: 10 or 2: 1. The composition has a remarkable synergistic effect, the use dosage of the active ingredients can be effectively reduced, and generation of drug resistance of germs is slowed down.

Description

technical field [0001] The invention belongs to the field of pesticides and fungicides, in particular to the screening and application of medicaments for preventing and controlling Corynebacterium tobacco leaf spot. Background technique [0002] Tobacco is an important economic crop in my country. Tobacco leaf spot is one of the important diseases that cause yield reduction of tobacco leaves. C. cassiicola is one of the pathogens causing tobacco leaf spot. [0003] Fludioxonil, also known as Fludioxonil, Celebrex, etc. The common English name is fludioxonil, the molecular formula is C12H6F2N2O2, the relative molecular mass is 248.18, and the CAS number is 13141-86-1. chemical pesticides for disease control. Its mechanism of action is by inhibiting glucose phosphorylation-related transfer and inhibiting the growth of fungal mycelium, ultimately leading to bacterial death. Unique mechanism of action, no cross-resistance with existing fungicides. FRAC, the International Act...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/36A01N47/38A01P3/00
CPCA01N43/36A01N47/38Y02A50/30
Inventor 潘月敏孙圣楠羊国根鞠玉亮孙光军孙红权金祥卢志刚
Owner ANHUI AGRICULTURAL UNIVERSITY
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