Application of piperlongumine in preventing and treating plant pathogenic fungi

A technology of plant pathogenic fungi and piperamide, which is applied in the directions of application, plant growth regulator, botanical equipment and method, etc., can solve problems such as unreported piperamide compounds and the like

Active Publication Date: 2021-12-24
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the research on the anti-tumor activity of Piperamide is the most extensive at present, ...

Method used

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  • Application of piperlongumine in preventing and treating plant pathogenic fungi
  • Application of piperlongumine in preventing and treating plant pathogenic fungi
  • Application of piperlongumine in preventing and treating plant pathogenic fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Application of embodiment 1-piperamide in the prevention and treatment of plant diseases

[0029] 1. The source of Piperamide

[0030] Piperlongumine, Piperlongumine, CAS No. 20069-09-4, purity greater than 98%, purchased from Chengdu Effa Biotechnology Co., Ltd.

[0031] 2. Determination of inhibitory activity of Piperamide to phytopathogenic fungi

[0032] (1) The strains to be tested

[0033] Fusarium oxysporum f.sp cuben, Botrytiscirerea, Fusarium graminearum, Thanatephorus cucumeris, Alternaria alternata, R. blight of cotton ( Rhizoctomiasolani), Peronophytoora litchii, Exserohilum turcicum, Corynespora cassiicola, all of the above pathogens were tested by the Institute of Pesticide Chemical Biology, Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences Provided with applied subject groups.

[0034] (2) Test method

[0035] ①In vitro activity test

[0036] For the agricultural bactericidal activity of Piperamide, the...

Embodiment 2

[0044] The pot experiment of embodiment 2-tomato gray mold

[0045]The present invention uses Piperamide as the bacteriostatic active ingredient, carries out the living potted experiment of tomato cinerea, test method reference (Liu Xia, Li Qian, Wang Yonghong, etc. the bacteriostatic activity of metabolites of Xenobacterium nematophila YL001 bacterial strain .Plant Protection Journal), with a slight improvement, the tomato seeds were sown in flowerpots after germination, and the test began when 3 to 5 true leaves grew. Protection and therapeutic effect all establish 3 concentration treatments, namely 500, 250 and 125mg / L, every treatment repeats 3 times, every 3 pots are a treatment, meanwhile, set 50% iprodione wettable powder (200mg / L) as Drug control, clear water as blank control.

[0046] (1) The method for measuring the protective effect is as follows: spray the test liquid first, and inoculate the spore suspension of Botrytis cinerea after 24 hours.

[0047] (2) The m...

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Abstract

The invention provides application of piperlongumine in preventing and treating the plant pathogenic fungi. The test concentration of the piperlongumine serving as a bactericide for preventing and treating the plant pathogenic fungi is larger than or equal to 200 mg/L; as an antibacterial active component, the piperlongumine can be used for preventing and treating fusarium wilt, botrytis cinerea, gibberellic disease, physalospora piricola, alternaria alternata, peronophythora, northern leaf blight and corynespora fallen leaf, the inhibition rate is 80% or above, and meanwhile, the piperlongumine also has relatively strong protection and treatment effects on botrytis cinerea. Tests show that the piperlongumine has a broad-spectrum bactericidal effect on plant pathogenic fungi, and a foundation is laid for further utilizing piperaceae plant piper longum to create a novel botanical fungicide.

Description

technical field [0001] The invention relates to the technical field of application of botanical fungicides, in particular to the application of Piperamide in the prevention and treatment of plant pathogenic fungi. Background technique [0002] Piperlongumine (piperlongumine), also known as piperlongumine, belongs to amides alkaloid compounds. Its chemical structure is shown below, and its molecular formula is C 14 h 19 NO 5 , with a relative molecular mass of 317.3410, mainly exists in the roots of Piperaceae plants. [0003] In recent years, with the continuous deepening of research, studies have shown that Piperamide has important medicinal value, including for the treatment of tumors, spleen diseases, chronic malaria, viral hepatitis, cough, stomach pain, asthma, bronchitis, Diarrhea, respiratory infection, tongue paralysis and gonorrhea etc. In addition, recent studies have also found that Piperamide has anti-anxiety, anti-angiogenic, anti-platelet aggregation, anti-...

Claims

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Application Information

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IPC IPC(8): A01N43/40A01P3/00
CPCA01N43/40Y02A50/30
Inventor 冯岗张静朱发娣叶火春闫超
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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