Application of compound H2 in preparation of lipopeptide bacteriostatic preparation

Abstract

The invention provides application of a compound H2 in preparation of a lipopeptide bacteriostatic preparation. The structural formula of the compound H2 is shown as a following formula (please see the formula in the description). The compound H2 is obtained by studying and separating the specific active ingredients of fermentation liquid of bacillus amyloliquefaciens HAB-2, has the wide activity on inhibiting tropical pathogenic fungi, has the effect of promoting growth of some plants, has the preservation effect on fresh fruits, can be applied to preparation of the lipopeptide bacteriostatic preparation and prevention and control of plant fungus diseases, particularly can be applied to tomato early blight, watermelon fusarium wilt, banana fusarium wilt, cotton fusarium wilt, rubber tree anthracnose, a rubber tree corynespora leaf fall disease, tea grey blight, mango anthracnose and/or a rubber tree brown root rot disease and the like and is worthy of application and popularization.

Description

technical field [0001] The invention belongs to the field of compounds, and in particular relates to the application of compound H2 in the preparation of lipopeptide antibacterial preparations. Background technique [0002] Bacillus is a kind of Gram-positive strain that produces spores. It is widely distributed, rich in physiological characteristics, easy to isolate and cultivate, and is an important microbial flora in nature. Bacillus can produce a variety of antibacterial active substances, including Non-ribosomal peptide antibiotics synthesized by ribosomal pathway and bacteriocins, enzymes and other active protein antibacterial substances synthesized by ribosomal pathway. [0003] Strain 168 is the model strain of Bacillus and the most thoroughly studied strain at present. Its whole genome was sequenced in 1997. Different from the wild Bacillus, the model strain 168 lost many wild-type characteristics due to the long-term subculture in the laboratory, such as the spont...

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Problems solved by technology

[0004] The target strain of this study is Bacillus amyloliquefaciens HAB-2 (the strain was preserved in the China Type Culture Collection Center on January 27, 2015, and proved to be alive. Its preservation registration number is: CCTCCM2015070, and the address is in Wuhan University) A series of preliminary experiments showed that the biocontrol bacterium HAB-2 has a broad-spectrum activity of inhibiting tropical pathogenic fungi, and can promote the growth of some plants and keep fresh fruits. However, this strain does not have the sfp gene, but through modern With the instrumental analysis method, we have detected the lipopeptide substances—surfactin substances that cannot be obtained due to the lack of sfp in the HAB-2 fermentation broth. Therefore, it is necessary to study the specific active ingredients of this strain, and it is worth further research. Research

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