Probiotic active soybean milk beverage and preparation method and application thereof
A technology of probiotics and soybean milk, applied in the direction of bacteria, application, and lactobacilli used in food preparation, can solve the problems of single soybean milk products, short storage time, and difficulty in digesting, so as to improve sleep quality, strong fresh-keeping ability, The effect of improving peristalsis
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Embodiment 1
[0035] 1. Strain selection
[0036] 1. Select Lactobacillus plantarum subsp.plantarum, strain collection preparation: CICC 10345, purchased from China Industrial Microorganism Culture Collection Management Center.
[0037] 2. Verify whether Lactobacillus plantarum can produce bacteriocin
[0038] (1) After activation of Lactobacillus plantarum subsp. plantarum, inoculate in CM0006MRS solid medium, culture at 35°C, pH7.5, transfer and purify the culture dish, pick a single colony of the bacteria with an inoculation loop and inoculate in CM0006MRS In the liquid culture medium, shake culture at 35°C for 48 hours, centrifuge the culture solution at 3000r / min, 4°C for 10min, remove the bacteria, keep the supernatant as the fermentation supernatant of the bacteria, and use the acid precipitation method to prepare the crude extract of the bacteria white;
[0039] CM0006MRS medium formula: casein peptone 10.0g, beef extract 10.0g, yeast powder 5.0g, glucose 5.0g, sodium acetate 5.0g...
Embodiment 2
[0053] This embodiment provides a probiotic active soymilk beverage, which is basically the same as that of Embodiment 1, except that:
[0054] In S1, the dosage of each raw material is: 60g of soybeans, 5g of carrots, 15g of white sugar, 6g of fried wild jujube kernels, and 390ml of water;
[0055] In S2, the inoculum amount of Lactobacillus plantarum subsp. plantarum was 1%, the fermentation temperature was 30° C., the fermentation time was 2.5 hours, and the pH was 4.8.
Embodiment 3
[0057] This embodiment provides a probiotic active soymilk beverage, which is basically the same as that of Embodiment 1, except that:
[0058] In S1, the dosage of each raw material is: 80g of soybeans, 6g of carrots, 20g of white sugar, 8g of fried jujube seeds, and 570ml of water;
[0059] In S2, the inoculum amount of Lactobacillus plantarum subspecies is 2%, the fermentation temperature is 35°C, the fermentation time is 1.5h, and the pH is 5.4.
[0060] The mouthfeel, color, and smell of the probiotic active soymilk beverage prepared in Examples 1-3 were described by sensory evaluation, and 30 volunteers were selected for evaluation and scoring, and the average score was the final score of the sample. The sensory identification evaluation test was carried out according to the sensory evaluation principles, and the sensory evaluation principles are shown in Table 2.
[0061] Table 2 Sensory evaluation criteria of probiotic active soy milk beverage
[0062]
[0063] ...
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