Application of compound h2 in the preparation of lipopeptide antibacterial preparations
A technology of compound and lipopeptide, applied in the application field of preparation of lipopeptide bacteriostatic preparation
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[0032] Referring to the accompanying drawings, the present invention will be further described in conjunction with specific embodiments, so as to better understand the present invention.
[0033] 1. Compound separation
[0034] 1.1 Fermentation broth of Bacillus amyloliquefaciens HAB-2
[0035] Inoculate the preserved biocontrol bacteria strain HAB-2 on the lysing broth medium for activation at 28°C, cultivate for 3 days, pick out the soybean grain-sized biocontrol bacteria HAB-2 and add it to the liquid lysing broth medium, Shake the bacteria at 28°C and 200 rpm for 2 days to obtain the fermentation broth. Use a high-speed centrifuge and centrifuge at 10,000 rpm for 10 minutes to remove the bacteria and obtain a sterile fermentation broth.
[0036] 1.2 Butanol extraction method
[0037] Fully mix the fermented liquid and n-butanol at a ratio of 1:1 by volume, stand still for 12 hours, extract with a separatory funnel to obtain n-butanol in the upper layer, and use a rotary ...
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