Solid culture medium for improving spore production of Corynespora fungi and culture method

Abstract

The invention relates to a solid culture medium for improving the spore production of Corynespora fungi. The solid culture medium is composed of the following raw materials in parts by weight: 2-8 parts of water, 2-8 parts of soybean powder, 2-8 parts of mung bean powder, 2-8 parts of corn powder and 2-8 parts of wheat bran. A culture method of the solid culture medium includes the steps that: Corynespora fungi are cultured on Sabouraud medium for 15 days, then conidias are eluted with 0.6-1% Tween 80 to make a spore suspension, the concentration of the spore suspension is adjusted to 1x10<6> / ml, and then inoculated into Sabouraud medium without agar, and shake culturing is carried out in a shaker at 27 DEG C and at a speed of 200 r / min for 48h to obtain a seed liquid, the seed liquid is inoculated into a solid culture medium, even stirring is performed, and then culturing is performed in an incubator at 27 DEG C for 15 days to obtain a finished product, namely the solid culture medium. The solid culture medium of the invention has simple raw material and low cost, and the spore yield is up to 4.493x10<10> spores / g after the solid culture medium is adopted to culture Cladosporium fungi.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a solid medium and a culture method for improving the sporulation yield of I. clavulata fungus. Background technique [0002] At present, in my country, there are about 16 species of Isaria fumosorosa recorded, among which Isaria fumosorosea, Isaria cicadae and Isaria cicadae are the most common and most studied. Isariafarinosa) et al. (ZimmermannG, 2008). Among them, I. fumoscens has been used in the biological control of greenhouse pests in North America and Europe. In the prior art, related fungal insecticide products have been registered for greenhouse whitefly (Aleyrodidae), aphids (Aphidoidea), Control of thrips and scale insects (Coccoidea) (Meng Xiangyun, 2011). Isaria sp. belongs to the genus Isaria sp., belongs to the subphylum Pezizomycotina, the class Sondariomycetes, the order Hypocrellales, and the family Conlycipitaceae. -1 belongs to the same gen...

AI Technical Summary

Problems solved by technology

[0003] At present, the sporulation yield of the medium used for cultivating I. clavves is not ideal. It has been reported that the best medium for I. fumigatus is PDA medium, and the optimum temperature for mycelial growth is 30°C. The best sporulation temperature is 25°C (Tian Jing et al., 2014); it has also been reported that a solid-liquid two-phase culture method was used to inoculate I. fumigatus into a Sa-type liquid medium and then inoculate it as a mother liquor into 11 % wheat bran and 76% cottonseed hulls in solid medium, the sporulation can reach up to 2.15×10 9 spores / g (Cao Na, 2018), etc.

The sporulation yield of these culture media for cultivating I. clavulata fungus is not ideal, so there is an urgent need for a culture medium that is beneficial to improving the sporulation yield of I. clavespora fungus

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