Mycobacterium tuberculosis detection liquid phase chip and method for making same

A technology of Mycobacterium tuberculosis and detection solution, which is applied in the field of medicine and biology, can solve the problems of low sensitivity and specificity, and achieve the effects of improved sensitivity, good stability, and simple and easy preparation method

Inactive Publication Date: 2008-07-09
SUREXAM BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0019] Aiming at the low sensitivity and specificity of current methods for detection of Mycobacterium tuberculosis and evaluation of anti-tuberculosis curative effect and the defect that only one antigen can be detected in one reaction, the technical problem to be solved in the present invention is to provide a Mycobacterium tuberculosis detection solution Phase chip, the liquid phase chip simultaneously detects ESAT-6, CFP-10, 38kD protein, 16kD protein, LAM, Ag85B, MPT-64 and TBGL eight Mycobacterium tuberculosis-specific secreted antigens in serum

Method used

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  • Mycobacterium tuberculosis detection liquid phase chip and method for making same
  • Mycobacterium tuberculosis detection liquid phase chip and method for making same
  • Mycobacterium tuberculosis detection liquid phase chip and method for making same

Examples

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Embodiment 1

[0068] Example 1 Preparation of liquid phase chip for rapid detection of Mycobacterium tuberculosis and detection of antigen

[0069] The capture antibodies of the present invention are monoclonal antibodies that can specifically bind to ESAT-6, CFP-10, 38kD protein, 16kD protein, LAM, Ag85B, MPT-64 and TBGL respectively; the detection antibodies are respectively Monoclonal antibody or polyclonal antibody that can specifically bind to ESAT-6, CFP-10, 38kD protein, 16kD protein, LAM, Ag85B, MPT-64 and TBGL. The "ESAT-6, CFP-10, 38kD protein, 16kD protein, LAM, Ag85B, MPT-64 and TBGL" recombinant proteins used in this example were purchased from British SCiPAC company, "ESAT-6, CFP-10, 38kD protein, The capture antibody and detection antibody of 16kD protein, LAM, Ag85B, MPT-64 and TBGL" were purchased from abcam company in the United States.

[0070] In the present embodiment, the formula of described various solutions is as follows:

[0071] 1.50mM MES buffer (pH5.0) formula...

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Abstract

The invention discloses a liquid phase chip for rapidly detecting mycobacterium tuberculosis, which mainly comprises a coated microsphere; detection antibodies respectively marked by biotins; and streptavidin phycoerythrin. The liquid phase chip for detecting specific antigen secreted by mycobacterium tuberculosis has the advantages of high detection efficiency, less sample size, high specificity, high sensitivity, etc. Meanwhile, markers specific secretory antigen secreted by mycobacterium tuberculosis can be combined freely and have convenient usage. The liquid reaction environment facilitates keeping the natural conformation of proteins, so that the reaction between a probe and an object to be detected is faster and completer, thus greatly improving the detection sensitivity and the linear range.

Description

technical field [0001] The invention belongs to the category of medical biology, and in particular relates to a liquid-phase chip for detecting mycobacterium tuberculosis and a preparation method thereof. Background technique [0002] Tuberculosis, caused by Mycobacterium tuberculosis (TB), is one of the most prevalent human infectious diseases in the world today. About 1 / 3 of the world's population and about 2 billion people have been infected with Mycobacterium tuberculosis (MTB). There are 9 million new TB cases and about 3 million deaths from TB every year. There are more than 6 million tuberculosis patients in my country, and at least 1.13 million new tuberculosis cases occur every year, and the annual death toll due to tuberculosis is as high as 250,000, and 75% of tuberculosis cases occur among young adults aged 15-50, seriously affecting the my country's national economy and population quality development. Accurate and timely diagnosis of active pulmonary tuberculo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/546G01N33/533
Inventor 许嘉森林一群杨惠夷
Owner SUREXAM BIO TECH
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