Method for co-extracting DNA/RNA (Deoxyribonucleic Acid/Ribonucleic Acid) virus nucleic acid
A RNA virus and co-extraction technology, which is applied in the field of rapid co-extraction of nucleic acid from respiratory specimens based on magnetic beads, can solve the problems of cumbersome steps, great harm to human body, and reduced amount of nucleic acid, and achieve good application prospects and accurate and reliable results
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[0044] Example 2 Detection of housekeeping genes GAPDH and HTN3
[0045] The GAPDH gene is an essential gene to maintain the minimum function of human cells, that is, the housekeeping gene. The amount of DNA in the nucleic acid extracted from the throat swab can be measured by detecting the amount of the GAPDH gene, and the HTN3 gene (Histatin 3) is the saliva The specific marker of mRNA can measure the amount of RNA in the nucleic acid extracted from the throat swab by detecting the amount of the HTN3 gene. The gene sequence is shown in Table 3.
[0046] (1) Co-extraction of housekeeping gene nucleic acid from throat swab specimens
[0047] (1) Sample preparation: Collect 5 throat swab specimens from healthy people, add 1ml of sterilized physiological saline to a sterile tube, shake and mix well, squeeze the cotton swab dry, and immediately transfer all the liquid to a 1.5ml centrifuge tube, Take 200μl and add it to another 1.5ml centrifuge tube, centrifuge for 5min, and disca...
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