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Application of dicycloplatin in preparation of antiviral drug and antibacterial drug

A technology of antiviral and antibacterial drugs, applied in the direction of antibacterial drugs, antiviral agents, active ingredients of heavy metal compounds, etc., can solve problems such as serious physical injury of patients

Active Publication Date: 2014-11-05
北京默加农生物技术发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, in order to kill cancer cells, it is usually necessary to carry out continuous administration within a certain period of time and maintain periodic treatment, but large doses and long-term treatment will cause great harm to the patient's body during the process of killing cancer cells
There are no reports showing that dicycloplatin can be used as an antibacterial and antiviral drug or as an adjunct to antibacterial and antiviral drugs

Method used

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  • Application of dicycloplatin in preparation of antiviral drug and antibacterial drug
  • Application of dicycloplatin in preparation of antiviral drug and antibacterial drug
  • Application of dicycloplatin in preparation of antiviral drug and antibacterial drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 dicycloplatin anti-phage test

[0022] 1. Test materials

[0023] Phage: Phage E13, provided by the Fifth Institute of Military Science;

[0024] Dicycloplatin injection: 10mg / mL, provided by Beijing Xingda Science System Company;

[0025] Indicator bacteria: Escherichia coli;

[0026] Indicator culture medium: LB medium;

[0027] Positive control drug: ribavirin, 10mg / mL.

[0028] 2. Inhibition of indicator bacteria by dicycloplatin

[0029] The bicycloplatin injection was serially diluted with sterile distilled water to make a concentration of 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 、10 -8 、10 -9 dicycloplatin diluent (concentration unit: mg / mL); Dicycloplatinum is diluted with distilled water to avoid Cl in the culture medium or solution - , EDTA, and pH value adversely affect the antiviral and / or antibacterial effects of dicycloplatin, thereby making the test results more accurate.

[0030] Take 0.9mL of dicycloplatin diluent of e...

Embodiment 2

[0036] Example 2 Dicycloplatinum anti-bimycobacterium test

[0037] 1. Test materials

[0038] Strains: Mycobacterium tuberculosis H 37 Rv, drug-resistant Mycobacterium tuberculosis (i.e., drug-resistant Mycobacterium tuberculosis strains resistant to isoniazid, streptomycin sulfate, rifampicin, and ethambutol), fast-growing nontuberculous mycobacteria, all from Virology State Key Laboratory (Wuhan University);

[0039] Dicycloplatin solution: 5mg / mL, provided by Beijing Xingda Science System Company;

[0040] Culture medium for each strain: modified Roche medium;

[0041] Positive control drug: rifampicin, 5mg / mL.

[0042] 2. Dicycloplatin against mycobacteria

[0043] Separately culture the above-mentioned Mycobacterium tuberculosis H 37 After Rv, drug-resistant Mycobacterium tuberculosis, and non-tuberculosis Mycobacteria, a 1 mg / mL bacterial solution was prepared.

[0044] The bicycloplatin solution (5 mg / mL) was diluted with sterile distilled water to prepare dicyc...

Embodiment 3

[0058] Embodiment 3 bicycloplatin anti-hepatitis B virus test

[0059] 1. Test materials

[0060] Cells: HepG2.2.15, provided by the State Key Laboratory of Virology (Wuhan University);

[0061] Dicycloplatin injection: 10mg / mL, provided by Beijing Xingda Science System Company;

[0062] Positive control drug: lamivudine.

[0063] 2. Dicycloplatin against hepatitis B virus

[0064] Carry out gradient dilution to bisbicycloplatin injection with sterile distilled water, respectively make the concentration of 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 、10 -8 dicycloplatin diluent (concentration unit: mg / mL).

[0065] After the HepG2.2.15 cells were cultured to the logarithmic growth phase, they were digested with trypsin into a single cell suspension and diluted to 10 5 cells / mL, inoculated in 96-well cell culture plate, 100 μL per well, three duplicate wells for each concentration, cultured at 37°C for 24 hours, and then added 200 μL of dicycloplatin diluents of the abo...

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Abstract

The invention provides application of dicycloplatin in preparation of an antiviral drug and / or antibacterial drug and application of the dicycloplatin in preparation of an antiviral adjuvant drug and / or antibacterial adjuvant drug. Viruses related in the invention are RNA viruses such as hepatitis B virus, hepatitis C virus, human immunodeficiency virus and influenza virus, and the antibacterial drug is resistant to mycobacterium tuberculosis and / or nontuberculosis mycobacteria, Research results prove that dicycloplatin has the effect of resisting multiple bacteria and viruses, especially has obvious inhibitory effect on drug-resistance bacteria, no obvious cytotoxicity and high medication safety.

Description

technical field [0001] The invention relates to the application of dicycloplatinum, in particular to the application of dicycloplatinum in the preparation of antiviral drugs and antibacterial drugs. Background technique [0002] Dicycloplatin (Dicycloplatin) is a relatively stable supramolecular compound composed of carboplatin and cyclobutanedioic acid through four hydrogen bonds. ) and 1,1-cyclobutane dicarboxylic acid (Bis-[1,1-cyclobutane dicarboxylic acid diaminoplatin(Ⅱ)]complex). Studies have shown that bicycloplatin has the characteristics of broad spectrum, high efficiency, low toxicity, low drug resistance, low cross-resistance and good penetration, and is a new generation of supramolecular anticancer drugs. For example, the invention patent with the publication number CN1311183A has conducted multiple acute toxicity, drug efficacy experiments and clinical tests on dicycloplatin. It has significant curative effect on lung cancer, liver cancer, pancreatic cancer, ...

Claims

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Application Information

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IPC IPC(8): A61K31/282A61P31/18A61P31/16A61P31/14A61P31/20A61P31/04
Inventor 杨旭清邬开朗郑建强
Owner 北京默加农生物技术发展有限公司
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