Novel plant DNA virus carrier and its use

A DNA virus, a new type of plant technology, applied in the direction of plant genetic improvement, botanical equipment and methods, applications, etc., can solve problems such as laborious and time-consuming plant regeneration, low expression of foreign genes, and impact on the industrialization process of medicinal proteins.
CN1425772AInactive Publication Date: 2003-06-25ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2003-06-25
Estimated Expiration
Not applicable · inactive patent
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Abstract

The plant DNA virus carrier includes the infectious carrier of Chinese tomato yellowing curly leaf virus DNA-A and DNA-beta, derived plant gene silencing carrier and virus expressing carrier. The present invention also provides one new kind of satellite DNA molecule constructing plant DNA virus carrier and it has at least 72% homology with any one sequence of SEQ ID No.1-12. The present invention also provides the use and method of utilizing plant DNA virus carrier in virus functional genome research, plant functional genome research and foreign protein expression. The present invention provides one important platorm for researching virus genomee structure, function and expression and regulation mechanism and the interaction between virus and plant; provides one excellent tool for researching plant functional gene; and establishing one technological platform for using plant as bioreactor expressing foreign protein.
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Description

technical field

[0001] The present invention relates to a novel plant DNA virus vector and its application, specifically, the present invention relates to the infectious vector of Chinese tomato yellow leaf curl virus DNA-A and DNAβ and the plant gene silencing vector and virus expression vector derived from it . The invention also relates to a DNA beta satellite molecule for constructing a plant DNA virus vector and a whole genome sequence thereof. The invention also relates to an application and method of using the plant DNA virus vector to determine genome functions, plant genome functions and express foreign proteins. Background technique

[0002] Whitefly-transmitted geminivirus (WTG) is a kind of plant single-stranded DNA virus that occurs widely in the world. loss. WTG belongs to the Begomovirus genus (Begomovirus) in the Geminiviridae family (Geminiviridae) in taxonomy, including more than 100 independent species. Under natural conditio...

Claims

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