Method for extracting rhamnolipid product in fermentation liquid
A technology of rhamnolipid and fermentation broth, which is applied to the preparation of sugar derivatives, sugar derivatives, sugar derivatives, etc., can solve the problems of high toxicity, high cost, etc., and achieve the effect of quick extraction and avoidance of use.
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[0020] Example 1: Extraction of rhamnolipid products from fermentation broth using glucose as carbon source.
[0021] 1) Preparation of fermentation medium: weigh separately with an electronic balance, 30g glucose, 3g NaNO 3 , 1g KH 2 PO 4 , 2gK 2 HPO 4 ·3H 2 O, 0.4g MgSO 4 ·7H 2 O, 1g yeast powder; add 200ml of distilled water and mix well, pour it into a volumetric flask, distilled water to make the volume to 1L; add 30% NaOH, adjust the pH of the medium to 7.0; after dispensing into the Erlenmeyer flask, place Sterilize at 115°C for 30 min.
[0022] 2) Preparation of seed solution: 2 inoculation loops of slant strain of Pseudomonas aeruginosa ATCC27853, which produces rhamnolipid, inoculate into a Erlenmeyer flask containing 100ml LB medium, at 37°C, 180rpm / min , Incubate for 6-10 hours, to OD 600 The value is 0.7, that is, the seed liquid cultivated to the late logarithmic growth period is obtained, and the number of bacteria is 10 5 Pcs / ml.
[0023] 3) Preparation of fermentati...
Example Embodiment
[0027] Example 2: The selected strains, medium components, and culture conditions are the same as those in Example 1, except that the chitosan used has a different degree of deacetylation, and the degree of deacetylation is set to 85%, 90% and 92. In the experimental treatment of 3 kinds of chitosan, the method of the present invention is used to extract the rhamnolipids in the fermentation broth. The yields of rhamnolipids were 45.8%, 60.8% and 62.1%, respectively; the results showed that when the degree of deacetylation of chitosan was ≥90%, the yield of rhamnolipids was ≥60%.
Example Embodiment
[0028] Example 3: Extraction of rhamnolipid products from fermentation broth using soybean oil as carbon source
[0029] Prepare a fermentation medium with soybean oil as a carbon source, weigh it with an electronic balance, 3g NaNO 3 , 1g KH 2 PO 4 , 2g K 2 HPO 4 ·3H 2 O, 0.4g MgSO 4 ·7H 2 O, 1g yeast powder; add 200ml of distilled water and mix well, pour it into a volumetric flask, distilled water to make the volume to 1L; add 30% NaOH, adjust the pH of the medium to 7.0; after dispensing into the Erlenmeyer flask, follow The concentration is 30g / L. Soybean oil is added to the Erlenmeyer flask; then sterilized at 121℃ for 20min.
[0030] Rhamnolipid-producing Pseudomonas aeruginosa (Pseudomonas aeruginosa) DSM 22644 slant strain 2 inoculation loops, inoculate into the Erlenmeyer flask containing 100ml LB medium, culture 6-10 at 37℃, 180rpm / min Hours to OD 600 The value is 0.7, that is, the seed liquid cultivated to the late logarithmic growth period is obtained, and the number o...
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