Method for preparing phenylethanoid glycoside, mannitol, oligosaccharide syrup and polysaccharide from broomrape

A technology of phenylethanoid glycosides and oligosaccharide syrup, which is used in the preparation of oligosaccharides and sugar derivatives, chemical instruments and methods, etc.

Active Publication Date: 2015-01-07
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is still a large gap be...

Method used

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  • Method for preparing phenylethanoid glycoside, mannitol, oligosaccharide syrup and polysaccharide from broomrape

Examples

Experimental program
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Effect test

Embodiment 1

[0055] Take desert cistanche 100kg, crush through a 40-mesh sieve, add 1.5m 3 50% ethanol solution, 55 ℃ under ultrasonic enhanced extraction (ultrasonic power is 300W) 100min, extract 1 time, extract liquid 500 order filter cloth filter, filtrate decompression concentrates, obtains concentrated liquid I altogether 340L. Among them, the concentrated solution I was added to the macroporous adsorption resin HPD-300 column, the volume of the chromatographic medium was 170L, and the ratio of height to diameter was 8:1. 1.5 times the column volume per hour; then 6 times the column volume of 50% ethanol solution for elution, the flow rate is 1.5 times the column volume per hour, and the eluate is collected for future use.

[0056] The ethanol eluate was concentrated under reduced pressure at 55°C, and the concentrated vacuum was 0.6 MPa. The concentrated solution was vacuum-dried at 60°C, with a drying vacuum of 0.6 MPa, and the drying time was 24 hours, to obtain 5 kg of the phenyl...

Embodiment 2

[0061] Take desert cistanche 100kg, crush through a 10-mesh sieve, add 2m 3 70% ethanol solution, 20 DEG C under ultrasonic enhanced extraction (ultrasonic power is 3W / L) 60min, extract 1 time, extract liquid 500 order filter cloth filter, filtrate decompression concentrates, obtains concentrated liquid I altogether 340L. Among them, the concentrated solution I was added to the macroporous adsorption resin HPD-300 column, the volume of the chromatographic column medium was 170L, and the ratio of height to diameter was 8:1. It was eluted with deionized water 10 times the column volume, and the water eluate was collected at a flow rate of 1.5 times the column volume per hour, 6 times the column volume of 50% ethanol solution for elution, the flow rate is 1.5 times the column volume per hour, and the eluate is collected for future use.

[0062] The ethanol eluate was concentrated under reduced pressure at 70°C, and the concentrated vacuum was 0.6 MPa. The concentrated solution wa...

Embodiment 3

[0067] Take desert cistanche 100kg, crush through a 60-mesh sieve, add 0.8m 3 20% ethanol solution, 20 DEG C under ultrasonic enhanced extraction (ultrasonic power is 1000W) 60min, extract 3 times, extract liquid 500 order filter cloth filter, filtrate decompression concentrates, obtains concentrated solution I altogether 170L. Among them, the concentrated solution I is added to the macroporous adsorption resin HPD-300 column, the volume of the chromatography column medium is 170L, the ratio of height to diameter is 8:1, and it is eluted with deionized water of 10 times the column volume, and the flow rate is 0.2 times the column volume per hour. , collect the water eluate, elute with 50% ethanol solution of 6 times column volume, the flow rate is 0.2 times column volume per hour, collect the eluate for future use.

[0068] The ethanol eluate was concentrated under reduced pressure at 60°C, and the concentration vacuum was 0.7MPa; the concentrated solution was heated and dried...

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Abstract

The invention relates to a method for simultaneously preparing phenylethanoid glycoside, mannitol, oligosaccharide syrup and polysaccharide from broomrape, which comprises the following steps: extracting monosaccharide, oligosaccharide and phenylethanoid glycoside in broomrape by using an ethanol water solution, separating the phenylethanoid glycoside and saccharide with a macroporous adsorbent resin column, carrying out concentration and drying on the phenylethanoid glycoside solution obtained by elution to obtain the product, concentrating the saccharide under reduced pressure, crystallizing at normal temperature, separating the oligosaccharide and mannitol, recrystallizing the mannitol to obtain the high-purity mannitol, centrifugating the clear solution to obtain the oligosaccharide syrup, extracting the polysaccharide from residues by using pure water, and carrying out alcohol precipitation to collect the polysaccharide. The method enhances the comprehensive utilization ratio of the broomrape resources, simplifies the production technique, develops the phenylethanoid glycoside, mannitol, oligosaccharide syrup and polysaccharide products simultaneously, increases the economic added value of the broomrape product, and has favorable industrial production and market development prospects.

Description

technical field [0001] The invention relates to a method for simultaneously preparing phenylethanol glycosides, mannitol, oligosaccharide syrup and polysaccharides from Cistanche deserticola, belonging to the technical field of biochemical separation. Background technique [0002] Cistanche is a perennial plant belonging to the genus Cistanche (Cistanche Hoffmg.et Link) in the family Orobanchaceae. It obligately parasitizes the roots of Haloxylon plants, and there are about 22 species in the world. There are 4 species and 1 variant in China, mainly distributed in Inner Mongolia, Ningxia, Gansu, Qinghai and Xinjiang. Cistanche has long been used as a class of precious traditional Chinese medicine, also known as big yun, sweet big yun, cistanche, sweet cistanche, goblin, etc. It was first recorded in "Shen Nong's Materia Medica", and it has been recorded in detail in successive dynasties. Top-grade medicinal materials have the functions of warming and tonifying kidney yang, n...

Claims

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Application Information

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IPC IPC(8): C07H1/08C07H3/02C07H3/06C07H15/18C07C31/26C07C29/76C08B37/00
Inventor 赵兵郭元亨赵庆生
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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