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Preparation technology of soybean lecithin for injection

A technique for preparing soybean lecithin, which is applied in the field of preparation technology for soybean lecithin for injection, can solve the problems of low recovery rate of high-purity lecithin, large amount of eluent, and influence on product quality, and achieve low price and high product quality. Improved purity and stable product quality

Active Publication Date: 2010-08-04
NANJING UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the lecithin purity obtained by these techniques is higher, the amount of eluent used is too large, the elution time is long, and the recovery rate of high-purity lecithin is low
In addition, the use of alumina as a stationary phase cannot be regenerated, and lysophospholipids will be generated in column chromatography, which will affect product quality

Method used

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  • Preparation technology of soybean lecithin for injection
  • Preparation technology of soybean lecithin for injection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take 100g of concentrated soybean phospholipids, first use 300g of acetone to deoil at 40°C for 3 times, each time for 30min, filter, add 300g of n-hexane and 100g of isopropanol mixed solvent to the acetone insoluble matter to dissolve it. The mixed solution was passed through a polyacrylonitrile membrane with a molecular weight cut off of 15,000 at a temperature of 25° C. and a pressure of 0.38 MPa to obtain a permeate. Add 6.4 g of activated alumina to the permeate, conduct adsorption treatment at 35° C. for 60 min, filter, concentrate the filtrate under reduced pressure, and dry in vacuum to obtain crude lecithin. Through HPLC analysis, the content of phosphatidylcholine in the crude lecithin is 53.2%.

[0024] Take 120g of silica gel (100-200 mesh) soaked in chloroform, put it into a φ25mm×900mm glass chromatography column, weigh 4.5g of crude lecithin, dissolve it in 37g of chloroform, and load it with 400ml of 2:1 chloroform / Rinse with methanol, the flow rate i...

Embodiment 2

[0027] Take 80g of concentrated soybean phospholipids, first use 400g of acetone at 25°C to deoil twice, each time for 40min, filter, add 300g of n-hexane and 80g of isopropanol mixed solvent to the acetone insoluble matter to dissolve it. The mixed solution was passed through a polyvinylidene fluoride membrane with a molecular weight cut-off of 10,000 at a temperature of 35° C. and a pressure of 0.5 MPa to obtain a permeate. Add 2.56 g of activated clay to the permeate, decolorize it by adsorption at 50°C for 40 minutes, filter, concentrate the filtrate under reduced pressure, and dry it in vacuum to obtain crude lecithin. Through HPLC analysis, the content of phosphatidylcholine in the crude lecithin is 54.8%.

[0028]Take 90g of diatomite (300-400 mesh) soaked in dichloromethane, put it into a φ20mm×700mm glass chromatography column, weigh 3.7g of crude lecithin, dissolve it in 26g of dichloromethane, and load it with 300ml 4:1 dichloromethane / methanol flushing, the flow r...

Embodiment 3

[0031] Take 50g of soybean concentrated phospholipids, deoil with 250g of acetone at 25°C for 4 times, each time for 30min, filter, add 200g of n-hexane and 50g of isopropanol mixed solvent to the acetone insoluble matter to dissolve it. The mixed solution was passed through a polysulfone membrane with a molecular weight cut off of 20,000 at a temperature of 35° C. and a pressure of 0.5 MPa to obtain a permeate. Add 1.92 g of activated carbon to the permeate, conduct adsorption treatment at 45° C. for 120 min, filter, concentrate the filtrate under reduced pressure, and dry in vacuum to obtain crude lecithin. Through HPLC analysis, the content of phosphatidylcholine in the crude lecithin is 57.3%.

[0032] Take 80g of attapulgite (200-300 mesh) soaked in n-hexane, put it into a φ25mm×600mm glass chromatography column, weigh 2.5g of crude lecithin, dissolve it in 10g of n-hexane, and load it with 200ml of 1 : 1 n-hexane / ethanol flushing, the flow rate is controlled at 1.1ml / mi...

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Abstract

The invention relates to a preparation technology of soybean lecithin for injection. Soybean lecithin is taken as a raw material, crude lecithin is firstly obtained after the treatment of acetone deoiling, membrane separation, adsorption decolorization and the like, then the crude lecithin is undergone column chromatographic fractionation, filtration sterilization, decompression concentration and vacuum drying, and finally the soybean lecithin for injection is obtained. The invention adopts membrane separation to replace the traditional solvent extraction, so the operation is easy. A polar compound with surface hydroxyl groups is taken as a stationary phase, mixed solvent composed of weak polar solvent and strong polar solvent is firstly used as eluent to carry out gradient elution, after all the cephalin flows out, the strong polar solvent is taken as the eluent, thereby not only the separation selectivity is high, but also the usage of the eluent is greatly reduced, and meanwhile, the extraction efficiency of lecithin is high, the product quality is stable, and the industrial mass production is benefited.

Description

technical field [0001] The invention relates to extracting high-purity lecithin from soybean lecithin, in particular to a preparation process for extracting soybean lecithin for injection from soybean lecithin. Background technique [0002] Lecithin is an important substance that constitutes the human biofilm, and plays a key role in maintaining the physiological activity of the biofilm and the normal metabolism of the body. It has the functions of preventing arteriosclerosis, improving nervous tissue, and enhancing brain vitality. It is known as the "protector of cells" and "scavenger of blood vessels". And because the polar end containing phosphate radical and choline group in the lecithin molecule is hydrophilic, and the non-polar end composed of carbon-hydrogen bonds is lipophilic, this unique physical and chemical characteristics and physiological activity make it in It has very important application value in the pharmaceutical industry. At present, the biggest use of...

Claims

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Application Information

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IPC IPC(8): C07F9/10
Inventor 管国锋吴仁荣杨操高正松万辉郁丽薇
Owner NANJING UNIV OF TECH
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