Preparation technology of soybean lecithin for injection

A technique for preparing soybean lecithin, which is applied in the field of preparation technology for soybean lecithin for injection, can solve the problems of low recovery rate of high-purity lecithin, large amount of eluent, and influence on product quality, and achieve low price and high product quality. Improved purity and stable product quality

Active Publication Date: 2010-08-04
NANJING UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the lecithin purity obtained by these techniques is higher, the amount of eluent used is too large, the elution time is long, and the recovery rate of high-purity lecithin

Method used

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  • Preparation technology of soybean lecithin for injection
  • Preparation technology of soybean lecithin for injection

Examples

Experimental program
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Example Embodiment

[0022] Example 1

[0023] Take 100g of concentrated soybean phospholipids, firstly use 300g of acetone to deoil at 40°C for 3 times, 30min each time, filter, add 300g of n-hexane and 100g of isopropanol mixed solvent to dissolve the acetone insoluble matter. The mixed solution is passed through a polyacrylonitrile membrane with a molecular weight cut-off of 15000 under a pressure of 0.38 MPa at a temperature of 25° C. to obtain a permeate. 6.4 g of activated alumina was added to the permeate and treated for 60 min at 35° C., filtered, and the filtrate was concentrated under reduced pressure and dried under vacuum to obtain crude lecithin. After HPLC analysis, the content of phosphatidylcholine in crude lecithin was 53.2%.

[0024] Take 120g silica gel (100~200 mesh) soaked in chloroform, load it into φ25mm×900mm glass chromatography column, weigh 4.5g crude lecithin, dissolve it with 37g chloroform and load the sample, first use 400ml 2:1 chloroform / Methanol flushing, the flow r...

Example Embodiment

[0026] Example 2

[0027] Take 80 g of concentrated soybean phospholipids, first use 400 g of acetone to deoil at 25° C. for 2 times, 40 min each time, filter, and add 300 g of n-hexane and 80 g of isopropanol mixed solvent to dissolve the acetone insoluble matter. The mixed solution is passed through a polyvinylidene fluoride membrane with a molecular weight cut-off of 10,000 at a temperature of 35° C. and a pressure of 0.5 MPa to obtain a permeate. 2.56g of activated clay was added to the permeate to absorb and decolorize at 50°C for 40 minutes, filtered, and the filtrate was concentrated under reduced pressure and dried in vacuum to obtain crude lecithin. HPLC analysis showed that the content of phosphatidylcholine in crude lecithin was 54.8%.

[0028] Take 90g of diatomaceous earth (300-400 mesh) soaked in dichloromethane, load it into a φ20mm×700mm glass chromatography column, weigh 3.7g of crude lecithin, dissolve it with 26g of dichloromethane, and load the sample, first us...

Example Embodiment

[0030] Example 3

[0031] Take 50g of concentrated soybean phospholipids, firstly use 250g of acetone to deoil at 25°C for 4 times, 30min each time, and filter. Add 200g of n-hexane and 50g of isopropanol mixed solvent to dissolve the acetone insoluble matter. The mixed solution is passed through a polysulfone membrane with a molecular weight cut-off of 20,000 under a pressure of 0.5 MPa at a temperature of 35° C. to obtain a permeate. 1.92 g of activated carbon was added to the permeate and treated for 120 min at 45° C., filtered, and the filtrate was concentrated under reduced pressure and dried under vacuum to obtain crude lecithin. HPLC analysis showed that the content of phosphatidylcholine in crude lecithin was 57.3%.

[0032] Take 80g of attapulgite (200~300 mesh) soaked in n-hexane, load it into a φ25mm×600mm glass chromatography column, weigh 2.5g of crude lecithin, dissolve it with 10g of n-hexane, and load the sample, first use 200ml of 1 :1 N-hexane / ethanol flushing, ...

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Abstract

The invention relates to a preparation technology of soybean lecithin for injection. Soybean lecithin is taken as a raw material, crude lecithin is firstly obtained after the treatment of acetone deoiling, membrane separation, adsorption decolorization and the like, then the crude lecithin is undergone column chromatographic fractionation, filtration sterilization, decompression concentration and vacuum drying, and finally the soybean lecithin for injection is obtained. The invention adopts membrane separation to replace the traditional solvent extraction, so the operation is easy. A polar compound with surface hydroxyl groups is taken as a stationary phase, mixed solvent composed of weak polar solvent and strong polar solvent is firstly used as eluent to carry out gradient elution, after all the cephalin flows out, the strong polar solvent is taken as the eluent, thereby not only the separation selectivity is high, but also the usage of the eluent is greatly reduced, and meanwhile, the extraction efficiency of lecithin is high, the product quality is stable, and the industrial mass production is benefited.

Description

technical field [0001] The invention relates to extracting high-purity lecithin from soybean lecithin, in particular to a preparation process for extracting soybean lecithin for injection from soybean lecithin. Background technique [0002] Lecithin is an important substance that constitutes the human biofilm, and plays a key role in maintaining the physiological activity of the biofilm and the normal metabolism of the body. It has the functions of preventing arteriosclerosis, improving nervous tissue, and enhancing brain vitality. It is known as the "protector of cells" and "scavenger of blood vessels". And because the polar end containing phosphate radical and choline group in the lecithin molecule is hydrophilic, and the non-polar end composed of carbon-hydrogen bonds is lipophilic, this unique physical and chemical characteristics and physiological activity make it in It has very important application value in the pharmaceutical industry. At present, the biggest use of...

Claims

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Application Information

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IPC IPC(8): C07F9/10
Inventor 管国锋吴仁荣杨操高正松万辉郁丽薇
Owner NANJING UNIV OF TECH
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