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This invention discloses a method, apparatus, and system for back-drilling a circuit board. Based on a configured detection device and a drilling device, the method includes: acquiring the actual position information of each electroplated hole to be back-drilled on the circuit board using the detection device to determine the hole position deviation value; determining the actual center coordinates of the back-drilling based on the hole position deviation value; and determining the back-drilling depth of the corresponding electroplated hole; and performing back-drilling on the circuit board using the drilling device based on the actual center coordinates and back-drilling depth of each electroplated hole to be back-drilled. This invention achieves drilling position acquisition outside the processing step, resulting in lower data acquisition costs and higher efficiency. It eliminates the need to integrate hole position detection equipment next to the drilling machine spindle or to measure relevant data before drilling, thus improving work efficiency and reducing equipment complexity and cost.

The invention provides a fluorescent aptamer sensor for simultaneously detecting Hg < 2 + > and Pb < 2 + > in fruit and vegetable foods and an application method of the fluorescent aptamer sensor. The aptamer sensor takes a nucleic acid aptamer (Apt) for specifically recognizing the Hg < 2 + > and the Pb < 2 + > as a recognition unit, a fluorophore FAM labeled at the 5'end of the Apt as a donor, and a quenching group BHQ-1 labeled at the 3 'end of a complementary chain (CS) of the Apt as a receptor. When Apt and CS are hybridized, FAM and BHQ-1 are close to each other, fluorescence quenching is caused by a fluorescence resonance energy transfer effect, but when Hg < 2 + > or Pb < 2 + > exists, Apt forms a U-shaped structure or a G-quadruplex structure, hybridization of Apt and CS is prevented, the fluorescence resonance energy transfer effect is weakened, and fluorescence is recovered. The sensor has good linear response to Hg < 2 + > and Pb < 2 + > under the optimal condition, the detection limits are 11.40 nM and 1.15 nM respectively, Hg < 2 + > and Pb < 2 + > in fruit and vegetable food can be accurately and sensitively detected within 25 minutes, and the sensor has good accuracy, precision and specificity and is easy and convenient to operate, rapid and sensitive.

This invention belongs to the field of environmental and food safety testing technology, and specifically discloses a label-free Cd based on SYBR Green I dye. 2+ A rapid detection fluorescent aptamer sensor and its quantitative detection kit are designed to achieve highly sensitive and specific quantitative detection of cadmium ions in samples such as fruits, vegetables, and food products. The fluorescent aptamer sensor includes the nucleic acid aptamer Cd-(21) and its complementary strand CS. Cd‑(21) And SYBR Green I dye; this fluorescent aptamer sensor is used to detect Cd. 2+ This invention offers advantages such as simple operation, short processing time, high sensitivity, and good selectivity. It also provides a matching reagent kit containing the necessary aptamers, complementary strands, SGI, buffer solutions, and other reagents, facilitating operation and application. The kit is simple in design, suitable for various laboratory and field testing applications, and has broad application prospects. The label-free fluorescent aptamer sensor and its matching reagent kit of this invention are Cd... 2+ This provides a new solution for efficient detection, with good technical and practical application value.