Panax japonicus polysaccharides, preparation method and use thereof

A technology of bamboo ginseng polysaccharides and bamboo ginseng, which can be used in food preparation, application, allergic diseases, etc., can solve the problems of polysaccharides that cannot be extracted and curative effect restrictions, and achieve a simple and easy method, easy operation, and wide source of raw materials Effect

Inactive Publication Date: 2008-09-17
WUHAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if bamboo ginseng is directly used as traditional Chinese medicine, the curative effect is restricted, because bamboo ginseng contains a variety of polysaccharides, incl

Method used

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  • Panax japonicus polysaccharides, preparation method and use thereof
  • Panax japonicus polysaccharides, preparation method and use thereof
  • Panax japonicus polysaccharides, preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Use ethyl acetate and acetone to remove fat and small molecular impurities from the dried and crushed bamboo ginseng produced in Enshi, Hubei. After vacuum drying, take 100-1000 g of the dried solid and wash it with water for 2-5 times. After centrifugation, the precipitate is extracted with high-pressure hot water at 100-150°C for 2-4 times, each time for 0.5-1 hour. The extracted slurry was centrifuged again, and the solution was concentrated with a rotary evaporator, and a small amount of n-octanol was added to remove air bubbles during concentration. The concentrated solution was deproteinized by the Sevag method, then ammonia water was added to adjust the pH value of the solution to 7-10, and H 2 o 2 1 to 4 days for decolorization. After the solution is decolorized, it is then frozen at 0-50°C for 2-12 hours, and then melted at 0-80°C to denature and precipitate the starch, centrifuge and destarch the solution, and then dialyze the solution with tap water and dis...

Embodiment 2

[0022] The bamboo ginseng residue extracted with hot water in Example 1 was treated with concentrations of 0.1-2M NaOH and 0.01-0.1M NaBH at -10-75°C 4 The aqueous solution is stirred and extracted for 1-12 hours, and the solution is immediately neutralized with glacial acetic acid to a pH value of 6.5-7.5 after centrifugation. Repeat the extraction 2 to 6 times. When the solution was concentrated, n-octanol was added to defoam. After deproteinization, decolorization, and dialysis by the method in Example 1, polysaccharide samples RPS3 and RPS4 were obtained by freeze-drying. The transmission electron microscope images are as follows Figure 4 with Figure 5 shown.

Embodiment 3

[0024] With NaOH and NaBH in embodiment 2 4 The residue of Panax ginseng after aqueous extraction is soaked and extracted with 40-88% formic acid at -10-25°C for 0.5-2h, and after centrifugation, the clear liquid is neutralized with 20%-40% NaOH aqueous solution until the pH is 6.5-7.5. Repeat the extraction 2 to 3 times. After the solution was concentrated and left to stand, a large amount of salt crystallized, the salt crystallization was separated by centrifugation, the solution was decolorized and dialyzed, and then freeze-dried to obtain the polysaccharide sample RPS5. The transmission electron microscope picture is shown in Image 6 .

[0025] Table 1 shows the elemental analysis results of the polysaccharides of Panax ginseng prepared above. The weight average molecular weight of bamboo ginseng polysaccharide (M w ), molecular weight distribution (M w / M n ), molecular mean square radius of rotation (2 > z 1 / 2 ) and intrinsic viscosity ([η]) and other physical an...

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Abstract

The invention discloses panax japonicus polysaccharide.The main chain structure thereof is Beta-D-1 to 3-mannan and/or Alpha-D-3 to 4-mannan and heterosaccharide containing galactan, xylan and glucan, the weight-average molecular weight of the panax japonicus polysaccharide is 5,000 to 2,000,000, the intrinsic viscosity at 25 DEG C is 1 to 100ml/g, and the mole ratio of the three elements of C: H: O is 1: 2: 1. The preparation of the panax japonicus polysaccharide comprises the following steps: after being dried, smashed and skimmed, the panax japonicus is soaked by water under the normal temperature and is stirred for separation, the residue is extracted by high-pressure hot water, further extracted by alkali and reductive salt, and finally extracted by acid, and the panax japonicus polysaccharide which can be dissolved in water and DMSO and not be dissolved in methanol, ethanol and acetone is obtained by separation, neutralization by taking the solution, dehydration, protein removal, decolorization, desalination and drying. The panax japonicus polysaccharide which is prepared by the invention has higher anti-tumor activity and promotion of immune activity, which can be used for preparing drugs or health care products for improving immune function and anti-tumor. The method for preparing the product is simple and easy to operation, and the sources of the needed raw materials are wide.

Description

technical field [0001] The invention relates to a bamboo ginseng polysaccharide and its preparation method and application, which belong to the fields of polymer chemistry and physics, and also belong to the fields of biology, pharmacy, food and health products. Background technique [0002] Bamboo ginseng, or bamboo ginseng, white notoginseng, pearl ginseng, etc., is the bamboo whip-shaped rhizome of Araliaceae plant Panaxjaponicus C.A.Mey, which is mainly produced in central and southern my country. It is a traditional Chinese medicine in my country. . At present, the research on the chemical components contained in the rhizome of Panax ginseng is limited to Panax ginseng saponin, ginsenoside, notoginseng saponin R in domestic and foreign literatures. 2 and pseudoginsenoside F 11 , the polysaccharides of bamboo ginseng have not yet been studied. Bamboo ginseng is a special resource in our country. It is used as medicine, which has the functions of promoting blood circula...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P35/00A61P37/04A23L1/30A23L33/105
Inventor 黄志萍张俐娜
Owner WUHAN UNIV
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