Preparation method for two novel compounds with antitumor activity extracted from isodon excisoides and application of two novel compounds

A technology for simulating aroma-deficient tea vegetables and anti-tumor activity, applied in the field of medicine, can solve problems such as significant anti-tumor effect, and achieve the effects of strong orientation, high product purity, and fast separation speed.
CN107501097AActive Publication Date: 2017-12-22HENAN UNIV OF CHINESE MEDICINE
5 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HENAN UNIV OF CHINESE MEDICINE
Publication Date
2017-12-22

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to a preparation method for two novel compounds with antitumor activity extracted from isodon excisoides and an application of the two novel compounds. The novel compounds are extracted from the isodon excisoides and are applied to the preparation of antitumor drugs. The method comprises the steps: adding water into the isodon excisoides, carrying out boiling extraction, introducing an extract sample to a macroporous adsorption resin column, and carrying out gradient elution by using water and ethanol, so as to obtain Fr. A, Fr. B, Fr. C and Fr. D; subjecting part of Fr. C to gel column chromatography and methanol-water gradient elution, so as to obtain Fr. C1, Fr. C2, Fr. C3 and Fr. C4; and subjecting part of Fr. C2 to silica-gel column chromatography and petroleum ether-acetone and methanol gradient elution, so as to obtain Fr. B1-1, Fr. B1-2, Fr. C2a, Fr. C2b, Fr. C2c and Fr. C2d, subjecting Fr. C2c to methanol recrystallization so as to obtain an acetonitrile-water for white powder as eluate, carrying out semipreparative HPLC purification, collecting chromatographic peaks at 56.4min and 61.2min, separately placing products in a solvent, and carrying out anhydrous sodium sulfate dehydrating and nitrogen gas drying-up, thereby obtaining a compound 1 and a compound 2. The preparation method provided by the invention is easy in operation, high in orienting property, high in separating rate, high in efficiency and high in product purity and can be effectively used for preparing the antitumor drugs.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of medicine, in particular to a preparation method and application of a new compound with anti-tumor activity extracted from pseudo-fragrance-deficient tea vegetables. Background technique

[0002] The Camellia genus is considered to be the most promising medicinal plant for obtaining anti-tumor lead compounds. In recent years, a large number of new cytotoxic diterpenoids of Camellia have been discovered, and a number of compounds have entered research and development as anti-tumor candidate compounds stage, such as Rubescensin A, Eucalyptin A, Dayexiangchagenin, etc., the sales of related traditional Chinese medicine products are also increasing year by year, such as Three Sisters Tablets (Xiyexiangchacai), Winter Lingcao syrup, tablets (Donglingcao), anti-inflammatory and gallbladder tablets (containing Xihuangcao), etc. Isodon excisoides (Y.Z.Sun ex C.H.Hu) Hara, also known as wild perilla, was first recorded in "...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More