Fisetin with urate transporter 1 inhibitory activity as well as preparation method and application of fisetin
A technology for transporting protein and inhibiting activity, which is applied in the field of fisetin and its preparation, can solve problems such as no public reports, and achieves the effects of high purity, high activity and simple preparation method
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Embodiment 1
[0044] Embodiment 6, the influence of different extraction and purification conditions on the preparation of fisetin
[0045] (1) Effect of ultrasonic power on the preparation of fisetin
[0046] The results are shown in Table 1. When the ultrasonic power is 150W, the extraction rate and purity of fisetin are the highest.
Embodiment 2
On the basis of the above basic process, the ultrasonic extraction temperature was 20°C, 30°C, 40°C, 50°C, 60°C, and the other process conditions remained unchanged to study the influence of different ultrasonic temperatures on the preparation of fisetin.
[0048] The results are shown in Table 2. When the ultrasonic extraction temperature is 40°C, the extraction rate and purity of fisetin are the highest.
[0049] Table 2: Effects of different temperatures on the preparation of fisetin
Embodiment 3
[0050] The results are shown in Table 3: when the elution concentration of macroporous resin ethanol is 90%, the extraction rate and purity of fisetin are the highest.
[0051] Table 3: Effects of different ethanol elution concentrations on the preparation of fisetin
[0052] The results are shown in Table 3: when the gel column methanol elution concentration is 70%, the extraction rate and purity of fisetin are the highest.
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