Fisetin with urate transporter 1 inhibitory activity as well as preparation method and application of fisetin

A technology for transporting protein and inhibiting activity, which is applied in the field of fisetin and its preparation, can solve problems such as no public reports, and achieves the effects of high purity, high activity and simple preparation method

Active Publication Date: 2022-06-21
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at present, there is no public report on the method for preparing fisetin from licorice

Method used

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  • Fisetin with urate transporter 1 inhibitory activity as well as preparation method and application of fisetin
  • Fisetin with urate transporter 1 inhibitory activity as well as preparation method and application of fisetin
  • Fisetin with urate transporter 1 inhibitory activity as well as preparation method and application of fisetin

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 6, the influence of different extraction and purification conditions on the preparation of fisetin

[0045] (1) Effect of ultrasonic power on the preparation of fisetin

[0046] The results are shown in Table 1. When the ultrasonic power is 150W, the extraction rate and purity of fisetin are the highest.

Embodiment 2

On the basis of the above basic process, the ultrasonic extraction temperature was 20°C, 30°C, 40°C, 50°C, 60°C, and the other process conditions remained unchanged to study the influence of different ultrasonic temperatures on the preparation of fisetin.

[0048] The results are shown in Table 2. When the ultrasonic extraction temperature is 40°C, the extraction rate and purity of fisetin are the highest.

[0049] Table 2: Effects of different temperatures on the preparation of fisetin

Embodiment 3

[0050] The results are shown in Table 3: when the elution concentration of macroporous resin ethanol is 90%, the extraction rate and purity of fisetin are the highest.

[0051] Table 3: Effects of different ethanol elution concentrations on the preparation of fisetin

[0052] The results are shown in Table 3: when the gel column methanol elution concentration is 70%, the extraction rate and purity of fisetin are the highest.

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Abstract

The invention discloses fisetin with urate transporter 1 inhibitory activity and a preparation method and application thereof. The preparation method comprises the following steps: carrying out superfine grinding on licorice roots, and sieving to obtain superfine powder; soaking the superfine powder in ethanol, performing ultrasonic-assisted extraction, filtering and collecting filtrate, repeatedly extracting and filtering, and merging the filtrate to obtain a crude extract; concentrating the crude extract under reduced pressure to obtain an extract, redissolving the extract with water, extracting with an organic solvent, separating to obtain extract liquor, repeatedly extracting and combining the extract liquor; and carrying out macroporous resin coarse separation, chromatographic column fine grading and high performance liquid chromatography purification on the extract, and drying to obtain fisetin. The preparation method disclosed by the invention is simple and strong in specificity, the obtained fisetin has in-vitro urate transporter 1 inhibitory activity, and a new application of the glycyrrhiza uralensis fisetin is developed.

Description

technical field [0001] The invention belongs to the field of natural extracts, and in particular relates to fisetin with urate transporter 1 inhibitory activity and its preparation method and application. Background technique [0002] With the improvement of living standards and the increase of high-purine and high-protein diets, people's uric acid levels tend to increase, and the prevalence of hyperuricemia is also increasing year by year. Hyperuricemia is not only an important biochemical basis for diseases such as acute arthritis, tophi, kidney stones, and uric acid nephropathy, but also closely related to diseases such as coronary heart disease, hypertension, diabetes, heart failure, and chronic kidney disease. The pathogenesis of hyperuricemia is mainly abnormal purine metabolism and obstruction of uric acid excretion. [0003] At present, nearly 90% of clinical hyperuricemia is caused by decreased excretion of uric acid. About 2 / 3 of uric acid is excreted through the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30A61K31/352A61P19/06A23L33/105
CPCC07D311/30A61P19/06A23L33/105A23V2002/00A23V2200/30A23V2250/2116A23V2300/14A23V2300/38Y02A50/30
Inventor 徐锡明张京良王鹏王一帆
Owner OCEAN UNIV OF CHINA
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