43 results about "Uricotelic" patented technology

The invention discloses a urate oxidase mutant and application thereof in improving the thermal stability of urate oxidase, and belongs to the technical field of biology. The urate oxidase mutant disclosed by the invention is T68L / T75S / E222D / K299E, T68L / T75S / K299E or T75S / K299E, and the urate oxidase mutant disclosed by the invention is T68L / T75S / K299E. According to the method, the key sites influencing the thermal stability are mined in the modes of directed evolution, high-throughput screening, multiple virtual screening and the like, and the stability is improved through mutation. And finally, a plurality of key sites and mutants influencing the stability are excavated, and the thermal stability is improved.

The invention discloses uric acid-reducing peptide goat milk powder and a preparation method thereof, and belongs to the technical field of enzymes. According to the process for preparing the uric acid lowering peptide by hydrolyzing goat milk through the compound enzyme, the total addition amount of the compound enzyme (the enzyme activity ratio of neutral protease N to neutral protease X is 1: 1) is 4000 U / g-6000 U / g, enzymolysis is conducted for 2-3 h at the temperature of 45-55 DEG C, and under the condition, the xanthine oxidase inhibition rate is 71.67 + / -1.32%-83.64 + / -0.98%. The enzymolysis goat milk containing the uric acid-reducing peptide is subjected to spray drying to prepare the uric acid-reducing peptide goat milk powder, and the storage stability of the goat milk powder is relatively good. The amino acid sequence and the source of the uric acid lowering peptide are separated and identified, the in-vitro uric acid lowering activity of the uric acid lowering peptide is verified through the solid-phase synthesized uric acid lowering peptide, and a theoretical basis and technical support are provided for development of new uric acid lowering peptide goat milk products.

The application discloses a tetrapeptide IR4 with uric acid reducing activity, and a preparation method and application thereof, and belongs to the technical field of small molecular peptides. The amino acid sequence of the tetrapeptide IR4 is IDWR, and the tetrapeptide IR4 can be prepared by a solid-phase synthesis method and an enzymatic hydrolysis method. The tetrapeptide IR4 is identified from a porphyra haitanensis protease hydrolysate, has potential interaction with xanthine oxidase, and can reduce the uric acid content of zebrafish by 33.6% (to 5.93+ / -0.47 mu mol / g protein) at a concentration of 100 mu g / mL. Compared with an anserine (which can reduce the uric acid content of zebrafish by 25.7%), the tetrapeptide IR4 has better uric acid reducing activity. Compared with allopurinol (which can reduce the uric acid content of zebrafish to 4.61+ / -1.11 mu mol / g protein), the uric acid reducing ability of the tetrapeptide IR4 and the allopurinol has no significant difference. The tetrapeptide IR4 can be used for preparing uric acid reducing drugs and relieving hyperuricemia.

The invention relates to the technical field of preparation of deep-sea longevity fish precise active peptides, in particular to a deep-sea longevity fish precise active peptide with uric acid reducing and anti-inflammatory effects as well as a preparation method and application of the deep-sea longevity fish precise active peptide. The preparation method comprises the following steps: carrying out medium-temperature pretreatment, carrying out step-by-step enzymolysis with alkaline protease and acid protease, centrifuging, filtering, carrying out ultrafiltration interception and the like, and screening out 10 non-reported non-toxic active peptide fragments. The active peptide has a multi-target synergistic effect, inhibits the activity of xanthine oxidase, regulates and controls the expression of uric acid resorption protein, regulates and controls the expression of uric acid transporter protein, realizes bidirectional regulation of uric acid metabolism, efficiently removes hydroxyl radicals, activates superoxide dismutase and reduces the content of inflammation-related factors. The technology is simple and easy to control, low in equipment requirement and suitable for large-scale production, the active ingredients are natural, safety is high, the active ingredients can serve as functional factors to be applied to functional food, health care products and the like, and safe and diversified intervention schemes are provided for hyperuricemia and related inflammation problems.

The invention belongs to the technical field of biological fermentation, and particularly relates to a preparation method and application of a fermented rhizoma smilacis glabrae extract. The invention relates to a fermented rhizoma smilacis glabrae extract, which is characterized in that a lactobacillus rhamnosus LR.M8 strain is utilized, the inhibition effect of the rhizoma smilacis glabrae extract on xanthine oxidase is improved through fermentation, and the treatment effect of the rhizoma smilacis glabrae extract on hyperuricemia is improved. The solution for improving the effect of the extract through biological fermentation is provided for solving the problems that the content of effective components in common medicinal and edible extracts is low, and the human body utilization rate is not high. The lactobacillus rhamnosus LR.M8 can effectively improve the adjusting effect of the rhizoma smilacis glabrae medicine and food homologous plant extract on uric acid balance, can reduce damage of inflammation to cells, and promotes in-vivo purine metabolism to recover balance. The fermentation method provided by the invention is simple and remarkable in effect, and has a wide application prospect.

The application discloses a pentapeptide IK5 with uric acid reducing activity as well as a preparation method and application thereof, and belongs to the technical field of small molecular peptides. The amino acid sequence of the pentapeptide IK5 is ITGYK, and the pentapeptide IK5 can be prepared by a solid-phase synthesis method and an enzymolysis method. The pentapeptide IK5 is identified from a protease enzymolysis liquid of a fine weak red winged seaweed, has potential interaction with xanthine oxidase, and can reduce the uric acid content of zebrafish by 44.4% (to 5.93+ / -0.47 mu mol / g protein) at a concentration of 100 mu g / mL. Compared with ananeine (which can reduce the uric acid content of zebrafish by 25.7%), the pentapeptide IK5 has better uric acid reducing activity. Compared with allopurinol (which can reduce the uric acid content of zebrafish to 4.61+ / -1.11 mu mol / g protein), the uric acid reducing ability of the pentapeptide IK5 and the allopurinol has no significant difference. The pentapeptide IK5 can be used for preparing uric acid reducing drugs and relieving hyperuricemia.

The invention discloses a breeding method for intensively preventing and controlling goose gout and application of the breeding method, and aims to solve the technical problems of high incidence of goose gout and low survival rate in intensive goose breeding. According to the method, prevention and control are achieved through a cooperative scheme of graded disinfection, precise trace element supplement and environment regulation and control; 50-250 ppm hypochlorous acid is adopted for conducting graded sterilization on the goose house environment, a feeding trough and drinking water, and pathogen breeding and urate deposition inducements are cut off; when the geese are 15-75 days old, a German Q life water trace element solution is added into drinking water, the excretion function of the kidney is enhanced, and metabolic balance is adjusted; meanwhile, the breeding density is controlled to be 1-4 / m, the environment humidity is 55-70%, and ventilation and daily average activity amount are guaranteed. The method is accurate and safe in prevention and control and free of drug residues, the gout incidence rate of goose groups can be reduced to 4% or below, the survival rate is increased to 95% or above, the weight gain efficiency in the breeding period is increased by 10%-15%, the breeding cost and loss are remarkably reduced, the method is suitable for intensive breeding scenes of medium and small meat geese, breeding geese and the like, and comprehensive economic benefits are outstanding.

The invention relates to the field of bioelectrochemical sensors, in particular to an epitaxial gate type FET uric acid sensor and a uric acid concentration detection method. The FET uric acid sensor based on the epitaxial gate type comprises a field effect transistor and an epitaxial gate, the epitaxial grid electrode comprises ITO (Indium Tin Oxide) conductive glass connected with the grid electrode of the field effect transistor, an MXene / Ppy composite modification layer, a polypyrrole layer and a solution tank; the MXene / Ppy composite modification layer and the polypyrrole layer are sequentially deposited on the upper surface of the ITO conductive glass from bottom to top; the polypyrrole layer is provided with a specific imprinting cavity of uric acid to be detected; and the solution tank is arranged on the upper surface of the polypyrrole layer and is used for accommodating detection liquid. According to the invention, rapid, sensitive, accurate and low-cost detection of uric acid molecules in a low-concentration range can be realized.

This invention provides a traditional Chinese medicine composition for treating renal edema, pyelonephritis with ascites, and nephritis. It consists of over 20 herbs including Atractylodes lancea, Areca catechu, Saposhnikovia divaricata, and Alisma plantago-aquatica (specific formulation details can be found in the original text). It can be prepared as pills, granules, or decoctions for oral administration. Core efficacy: 1. Promotes diuresis and reduces swelling, eliminating renal ascites; 2. Clears heat and detoxifies, anti-inflammatory and antibacterial (synergistic effect on fungal infections and cervical erosion); 3. Regulates renal function and improves abnormal uric acid metabolism. Treatment plan: Mild cases: Use five basic formulas (①-⑤) composed of Codonopsis pilosula, Poria cocos, etc., taken three times daily; Severe cases: Use a potent diuretic formula containing Akebia trifoliata and Euphorbia pekinensis (one spoonful of pills twice daily); Treatment course is approximately one month, with a follow-up examination after three months. Advantages: Cure rate reaches 90%, low recurrence rate – pure traditional Chinese medicine preparation, no significant toxic side effects; also treats complications (fungal infection, cervical lesions). This treatment plan has been clinically verified through three generations and has significant efficacy in treating renal edema, ascites, and nephritis.

The invention belongs to the technical field of biological medicine, and particularly discloses a tea polyphenol self-polymerization nanoparticle for intracellular delivery of uricase and a preparation method and application thereof.The tea polyphenol self-polymerization nanoparticle comprises a tea polyphenol self-polymerization compound and uricase, the tea polyphenol self-polymerization compound is formed by preliminary self-polymerization of epigallocatechin gallate under catalysis of Mn < 2 + >, and the uricase is prepared from the tea polyphenol self-polymerization compound and the uricase; and mixing the polyphenol self-polymerized compound with uricase, and entrapping the uricase to obtain the tea polyphenol self-polymerized nanoparticles. The tea polyphenol self-polymerization nanoparticles have the functions of uricase combined delivery and synergistic treatment based on EGCG (epigallocatechin gallate) carriers (TPNs), the tertiary structure integrity of uricase can be effectively maintained, and the activity of uricase is ensured; meanwhile, uricase can be protected from being damaged by protease, and the stability of uricase is enhanced. Through the synergistic effect of uricase and TPNs in cells, both symptoms and root causes of intracellular urate crystallization and inflammatory reaction are treated, and a better scheme choice is provided for gout or hyperuricemia.

The invention relates to the technical field of protein drugs, in particular to a urate oxidase variant and a glycosylation modification form thereof. An engineered N-glycosylation site is introduced into urate oxidase, and a pair of engineered disulfide bonds is additionally introduced into a tetramer interface, so that the obtained glycosylation modified urate oxidase variant can improve folding stability, in-vivo performance and immunological properties on the basis of maintaining enzymatic activity.

This invention discloses a multi-channel microfluidic chip and device for detecting creatinine and uric acid. The invention is based on the principle that the H2O2 produced by the reaction of creatinine and creatinine hydrolase, and the H2O2 produced by the reaction of uric acid and uricase, rapidly quench the fluorescence of the aerogel. Sensitive detection of urinary creatinine and uric acid is achieved by detecting the fluorescence intensity. Because the sensor uses paper-based fiber material as a substrate and introduces quantum dot nanomaterials as sensitive units to construct a three-dimensional aerogel structure, it has characteristics such as a large specific surface area, providing more active sites for the adsorption of biological enzymes, thereby increasing the adsorption capacity. Simultaneously, the presence of a large amount of glutathione on the surface of the quantum dots constituting the aerogel provides good biocompatibility, which is beneficial for maintaining enzyme activity, thus improving the sensitivity and stability of the detection.

The invention belongs to the technical field of gene engineering, and particularly relates to a 3-ketosteroid-delta1-dehydrogenase mutant with improved heat resistance and an application of the 3-ketosteroid-delta1-dehydrogenase mutant. The 3-ketosterone-delta1-dehydrogenase AuKsdD gene derived from arthrobacter urate oxydans is subjected to structural analysis and site-directed mutagenesis design, and a mutant with higher catalytic activity and remarkably enhanced heat resistance compared with a wild type AuKsdD is obtained through directed mutation of key sites E244, K267, T301 and A335. The mutant can keep high catalytic efficiency for a long time under a high-temperature condition, and is suitable for industrial synthesis of steroid drug raw materials.

The invention discloses a stable lactic dehydrogenase and uric acid combined detection kit with strong anti-interference capability, and belongs to the technical field of detection. The joint detection kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 contains a buffer solution, a composite anti-interference agent, a composite stabilizer, L-lithium lactate, a compound surfactant, 4-aminoantipyrine, peroxidase and a preservative diazolidinyl urea; the reagent R2 contains a buffer solution, a coenzyme (NAD < + >), N-ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt uricase, a compound stabilizer, a compound surfactant and a preservative epsilon-polylysine hydrochloride. According to the present invention, the lactic dehydrogenase detection and the uric acid detection can be simultaneously achieved by one reagent, such that the use convenience is substantially improved, and the stability and the anti-interference ability of the reagent are further improved. The reagent disclosed by the invention is simple, convenient and rapid to operate, is suitable for automatic analysis, and is a lactic dehydrogenase and uric acid combined detection reagent which is more stable and strong in anti-interference capability.

The invention provides a method for screening a urate transporter inhibitor in vitro, and belongs to the technical field of biological detection. The invention constructs a cell model for expressing urate transporter, and provides a method for screening urate transporter inhibitors by a fluorescence detection method based on a microplate reader. According to the method, the screening efficiency is remarkably improved, an effective platform is provided, the method is used for systematically evaluating the inhibition effect of environmental pollutants on the urate transporter, the method has the advantages of being high in sensitivity and selectivity, easy and convenient to operate, safe and environmentally friendly, and more choices are provided for screening urate transporter inhibitors.

The present application relates to the technical field of edible and medicinal fungus fermentation, and discloses a fermentation method of Phellinus baumii with high Hispidin yield and application.The fermentation method of Phellinus baumii in the present application comprises inoculating Phellinus baumii strains into a culture medium containing copper ion compounds, and then fermenting to obtain the product.The mycelium of Phellinus baumii obtained by the fermentation method of the present application is rich in Hispidin, and can be widely used in the preparation of related drugs and health foods for liver protection, anti-tumor, anti-virus, oxidation resistance, uric acid reduction, etc.In addition, the present application also discloses a high-performance liquid chromatography detection method for analyzing the content of Hispidin, which can effectively analyze the content of Hispidin and its precursor substances (such as protocatechuic acid, protocatechuic aldehyde, coffee acid, and aspidin) in the fermentation product of Phellinus baumii, its derivative product, and the mycelium of Phellinus baumii, and is helpful to improve the accuracy of Hispidin detection.

The application belongs to the technical field of biotechnology, and relates to a kind of degradation of uric acid's casein lactobacterium YWC-01 strain, which can produce catalytic degradation of uric acid enzyme.The application also relates to a kind of degradation of uric acid's casein lactobacterium YWC-01 preparation, which contains the bacterial cell and / or crude enzyme of casein lactobacterium YWC-01 strain.The research results show that the casein lactobacterium YWC-01 strain and the enzyme produced by the casein lactobacterium YWC-01 strain provided by the application for biological degradation of uric acid are safe for human body, and can efficiently biodegrade uric acid, can remove all uric acid with initial concentration of 1g / L within 48 hours under the initial concentration of bacterial cell of 8x10 8 / mL; can remove all uric acid with initial concentration of 1g / L within 18 hours under the crude enzyme protein concentration of 0.8g / L.The casein lactobacterium YWC-01 strain and the enzyme produced by the casein lactobacterium YWC-01 strain have important application prospect in preventing and treating gout and corresponding complications.

The application discloses a black soldier fly growth promotion and chicken manure uric acid degradation synergistic regulation method, and relates to the technical field of machine waste biological treatment and intelligent sensing regulation. The application effectively strips the wide-spectrum noise generated by the violent mechanical crawling of the black soldier fly larvae by using the variational mode decomposition algorithm based on the growth age constraint, combining the introduced development stage correction factor and the biochemical feedback regulation coefficient, accurately extracts the core component reflecting the biochemical metabolism, and solves the problems of characteristic frequency drift and signal masking caused by biomass growth; and by constructing a nonlinear synergistic coupling regulation model, introducing the black soldier fly growth vigor factor and the synergistic adaptation balance index, the phase relationship between the larva feeding intensity and the microbial degradation dynamics is quantified; the proportional-differential predictive control logic is used to dynamically correct the enzyme dosage ratio, and the ammonia toxicity inhibition risk caused by the accumulation of uric acid products is effectively avoided.

The application provides a lactobacillus helveticus and application thereof in sugar-reducing and uric acid-reducing peptide lactobacillus goat milk, and raw materials are fresh goat milk or reconstituted goat milk, and the goat milk is fermented by lactobacillus helveticus KD12 which produces alpha-glucosidase inhibitor peptides and xanthine oxidase inhibitor peptides, so that the protein in the goat milk is decomposed into alpha-glucosidase inhibitor peptides and xanthine oxidase inhibitor peptides, and the sugar-reducing and uric acid-reducing peptide lactobacillus fermented goat milk with alpha-glucosidase inhibitor peptides and xanthine oxidase inhibitor peptides is obtained. The alpha-glucosidase inhibitor peptides and the xanthine oxidase inhibitor peptides are separated, purified and identified in amino acid sequences, the positions of the alpha-glucosidase inhibitor peptides and the xanthine oxidase inhibitor peptides in the goat milk protein are determined, sugar-reducing and uric acid-reducing peptides for sugar-reducing and uric acid-reducing peptide food are provided, and a reference for development of sugar-reducing and uric acid-reducing drugs is provided.

The invention discloses pentapeptide IK5 with uric acid reducing activity and a preparation method and application thereof, and belongs to the technical field of small molecule peptide. The amino acid sequence of the pentapeptide IK5 is ITGYK, and the pentapeptide IK5 can be prepared through a solid-phase synthesis method and an enzymolysis method. The pentapeptide IK5 is identified from a solieria tenuissima proteolysis solution, has potential interaction with xanthine oxidase, can reduce the uric acid content of zebra fish by 44.4% (reduced to 5.93 + / -0.47 [mu] mol / g protein) when the concentration is 100 [mu] g / mL, and has better uric acid reducing activity compared with goose carnosine (which can reduce the uric acid content of zebra fish by 25.7%). Compared with allopurinol (the uric acid content of zebra fish can be reduced to 4.61 + / -1.11 [mu] mol / g protein), the pentapeptide IK5 and allopurinol have no significant difference in uric acid reducing capacity, and the pentapeptide IK5 can be used for preparing uric acid reducing drugs and relieving hyperuricemia.

The invention relates to the field of microbial fermentation, in particular to fermented papaya pulp and a preparation method and application thereof. The invention provides a preparation method of fermented papaya pulp, which comprises the following steps: inoculating pretreated papaya pulp with plant lactobacillus, and fermenting to obtain the fermented papaya pulp, the preservation number of the plant lactobacillus is GDMCC 66229, and the preservation number of the plant lactobacillus is GDMCC 66229. The invention provides lactic acid bacteria fermented papaya pulp and a preparation method thereof. The fermented papaya pulp prepared by the method has a better uric acid reducing effect than that before fermentation.

The invention discloses application of intestinal bacteroides and metabolites thereof in treatment of colitis, and belongs to the field of microorganisms. The name of the bacterial strain is Bacteroides intestinae SCAUTCVM001, and the preservation number of the bacterial strain is GDMCC No: 67454. The invention further discloses a preparation method of the bacterial strain. The intestinal bacteroides SCAUTCVM001 disclosed by the invention can be used for metabolizing tryptophan (Trp) and indole-3-acetic acid (IAA) in vivo and in vitro to generate metabolites of yellow uric acid (Xa) and indole-3-formaldehyde (ICA); the mouse colitis can be relieved. Therefore, the intestinal bacteroides and the metabolite of the intestinal bacteroides can relieve mouse colitis, increase expression of colon ZO1 and IgA and treat mouse colitis.

The invention relates to an erythropalum scandens, cichorium intybus and plantain herb compound fermented uric acid reducing beverage and a preparation method thereof. The beverage is prepared from the following raw materials in dry weight ratio: 10-15 parts of erythropalum scandens, 8-12 parts of cichorium intybus, 5-10 parts of plantain herb and 3-5 parts of warm food materials. The warm food material is one or a combination of more of fresh ginger, red dates and longan; the beverage is prepared by an ultrasonic-assisted extraction and compound bacteria fermentation process, and compound bacteria are lactobacillus plantarum and saccharomycetes in a ratio of 2: 1. The activity of the erythropalum scandens phenolic component, the chicory triterpenes and the plantain herb flavone is improved by 30% or above after fermentation, the uric acid reduction rate of rats is larger than or equal to 20%, and the auxiliary uric acid reduction effect is remarkable; warm food materials neutralize cold nature, the problem that traditional heat-clearing beverages injure the spleen and the stomach is solved, the bitter taste is light, and the herbal fragrance is coordinated; ultrasonic extraction and compound bacteria fermentation are combined, so that the problem of plant fiber degradation is solved, and the fermentation efficiency is improved by 40% or above.

A postbiotic extract of Lactobacillus plantarum CB102 is prepared by the steps of: subjecting the Lactobacillus plantarum CB102 to a protein extraction treatment, so as to obtain a protein extract; and subjecting the protein extract to a hydrolysis treatment with a protease, so as to obtain the postbiotic extract of Lactobacillus plantarum CB102. The Lactobacillus plantarum CB102 is deposited at the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH under an accession number DSM 33894 in accordance with the Budapest Treaty. A method for preparing a postbiotic extract of Lactobacillus plantarum CB102, and methods for reducing a blood uric acid level and alleviating a uric acid metabolism-associated disorder using the postbiotic extract of Lactobacillus plantarum CB102 are also provided.

The application provides a fiber-based organic photoelectrochemical transistor uric acid sensor and a preparation method thereof. The fiber-based organic photoelectrochemical transistor uric acid sensor is obtained by growing Fe-MOF@PANI on the surface of a conductive fiber, then modifying uric acid oxidase and a biocompatible film solution as a gate electrode of the transistor, using the fiber loaded with a semiconductor material as a source-drain electrode of the transistor, and dropping a gel electrolyte. The organic photoelectrochemical transistor uric acid sensor uses a fiber-based material as an electrode, a photosensitive material and a sensing substance are modified on the gate electrode, the photosensitive material makes electrons jump to the surface of the electrode under light conditions, the channel current is regulated, and the sensing substance is used to detect uric acid. The uric acid sensor can work under zero gate bias, reduces the background signal and interference, shows a good current response to different concentrations of uric acid, and has great market promotion value.

The invention belongs to the technical field of biology, and particularly relates to regulation and control of a saussurea involucrata natural culture on a G quadruplex of a hyperuricemia related gene promoter region. According to the method, firstly, the influence of the saussurea involucrata natural culture on the G-quadruplex structure of the promoter region is evaluated by using a circular dichroism spectrum, and the ability of the saussurea involucrata natural culture in regulating and controlling the G-quadruplex of the hyperuricemia related gene promoter region is further explored by using a microplate reader; the support is provided for the molecular mechanism of the saussurea involucrata natural culture for relieving the hyperuricemia on the gene level. In conclusion, the invention discloses a brand new mechanism that the saussurea involucrata natural culture regulates and controls uric acid metabolism related genes through a targeted G quadruplex structure, a precise action target and a potential drug development direction are provided for treatment of hyperuricemia, and meanwhile, an established small molecule and G quadruplex interaction verification method provides technical reference for similar research.

The application belongs to the technical field of biology and relates to an enzyme protein, in particular to a recombinant Arthrobacter globiformis uricase and application thereof. The recombinant Arthrobacter globiformis uricase has a single-point amino acid mutation, and the amino acid is located in an active region and a non-active region of the uricase, and the amino acid sequence of the recombinant Arthrobacter globiformis uricase is shown in any one of SEQ ID NO: 1-8. The mutant uricase formed after the amino acid is introduced into the uricase has higher activity than the wild-type uricase, and in the case of effectively controlling the uric acid content, the use amount of the uricase can be more effectively reduced.

The invention discloses a supported double nano-enzyme based on nanoflowers, preparation of the supported double nano-enzyme and application of the supported double nano-enzyme in uric acid colorimetric detection. The supported double nano-enzyme is prepared by immobilizing peroxidase-like nano-enzyme in a uricase nanoflower structure. According to the invention, the peroxidase-like nano-enzyme is immobilized in a natural uricase nano-flower structure to construct the supported double nano-enzyme, so that the stability and the loading capacity of the peroxidase-like nano-enzyme are remarkably improved, and the transmission distance between a substrate and an intermediate product H2O2 in a cascade reaction is greatly shortened, thereby greatly improving the catalytic efficiency and the reaction rate. The method effectively overcomes the core problems of high dependence on natural enzyme, high cost, poor stability and low cascade reaction efficiency in the prior art. When being used for colorimetric detection of uric acid, the kit shows high sensitivity, good selectivity and anti-interference capability, and lays a solid foundation for developing portable uric acid detection equipment which is low in cost, high in performance and suitable for primary medical treatment and family use.

The present application belongs to the technical field of fermentation engineering. The present application provides a peach-based anti-uric acid and antioxidant fermented beverage and a preparation method thereof. The method comprises the following steps: (1) taking peach pulp, chitosan with a weight of 0.15-0.30% of the peach pulp, and loquat peel water extract with a weight of 0.15-0.25% of the peach pulp as a fermentation substrate, inoculating EcN for fermentation for 1 day to obtain a slightly fermented product; (2) inoculating Lactobacillus plantarum JYLP-326 and Pediococcus pentosaceus JYPR9330 for anaerobic fermentation for 5-7 days to obtain a fermented product; (3) centrifuging, taking the supernatant, and sterilizing to obtain the beverage. The present application first proposes a two-step fermentation process combining mild fermentation and deep fermentation, and introduces the synergistic combination of chitosan and loquat peel extract in the mild fermentation stage, thereby solving the problem that many easily browning fruit and vegetable raw materials including peach pulp are difficult to be prepared into anti-uric acid and antioxidant functional products through fermentation.