77 results about "Uricotelic" patented technology

The present invention provides a manganese metal-organic framework material with oxidase properties, a preparation method, and an application thereof. The material is prepared by the following method: using 1,3,5-trimethic acid as an organic ligand, manganese acetate tetrahydrate as a manganese source, and a water-ethanol solution as a solvent, to obtain a manganese metal organic framework (MOF) precursor via a solvothermal synthesis technique; the manganese MOF precursor is post-synthetically modified using an oxidant, wherein the oxidant is a mixed solution of an aqueous alkali metal hydroxide solution and H2O2, and the ratio of the manganese MOF precursor to the alkali metal hydroxide is (400-600) mg:(0.5-2) mmol. By using specific types and amounts of manganese MOF precursors and oxidants, the resulting metal-organic framework nanomaterial has specific enzyme-like properties, can exert the function of uricase, and has optimal and high uricase activity under neutral pH conditions.

The invention discloses application of laccase as a catalyst in catalytic synthesis of allantoin. According to the invention, laccase from thermophilus is used as an enzyme catalyst, uric acid is oxidized, hydrolyzed and decarboxylated to synthesize allantoin, and efficient biosynthesis of allantoin is realized under mild conditions; wherein the amino acid sequence of the laccase derived from the thermophilic fungi is as shown in SEQ ID NO. 1. The invention provides an efficient, environment-friendly and low-cost biosynthesis approach for biosynthesis of allantoin, and has industrial application prospects in biosynthesis of allantoin.

The present application relates to the technical field of microbial fermentation, and particularly relates to a universal fermentation device, a yogurt production system and a method for producing a yogurt with reduced uric acid. The universal fermentation device comprises a fermentation tank, a sampling tube, a test tube, a sliding member, a pressure detection module and a processor. The pressure detection module is installed on the test tube. The sliding member is slidingly fitted on the test tube, and the sliding member is connected with a first driving rod. The sliding member is provided with a clearance opening, and the clearance opening is matched with a plugging block. The plugging block is connected with a test rod and a second driving rod. One end of the sampling tube is communicated with the fermentation tank, and the other end is communicated with the test tube. The processor is used for determining the density of the fermentation sample and the viscosity of the fermentation sample. The universal fermentation device can realize continuous monitoring of the fermentation system during the fermentation process, effectively avoids sample confusion, improves the timeliness of analysis, ensures that the conditions reflected by the sample are highly consistent with the original fermentation system, and is suitable for various fermentation scenes.

The invention discloses a uric acid reducing composition containing eucommia polysaccharide as well as a preparation method and application thereof, and relates to the technical field of biological medicines. The uric acid reducing composition prepared by the invention is prepared from the eucommia polysaccharide, the mulberry leaf extract and the clove extract, and the eucommia polysaccharide, the mulberry leaf extract and the clove extract are compounded for use, so that generation-excretion dual blocking can be formed aiming at different links of uric acid metabolism, and multi-target synergistic uric acid reduction is realized. Meanwhile, plant-derived components are adopted, so that the safety is higher, and adverse reactions can be effectively reduced. Besides, lactobacillus sake, lactobacillus plantarum and kluyveromyces marxianus are adopted as fermentation strains to prepare the mulberry leaf extract through fermentation, and the content of effective components in the mulberry leaf extract can be effectively increased, so that the uric acid reducing effect of the prepared composition is improved.

The invention discloses a urate oxidase mutant and application thereof in improving the thermal stability of urate oxidase, and belongs to the technical field of biology. The urate oxidase mutant disclosed by the invention is T68L / T75S / E222D / K299E, T68L / T75S / K299E or T75S / K299E, and the urate oxidase mutant disclosed by the invention is T68L / T75S / K299E. According to the method, the key sites influencing the thermal stability are mined in the modes of directed evolution, high-throughput screening, multiple virtual screening and the like, and the stability is improved through mutation. And finally, a plurality of key sites and mutants influencing the stability are excavated, and the thermal stability is improved.

The invention discloses a urate oxidase mutant and application thereof in improving the heat stability of urate oxidase and the trypsin resistance, and belongs to the technical field of biology. The urate oxidase mutant disclosed by the invention is Y47F / T68L / T75W / D111G / K204F / F259I / K299E, T68L / T75W / K204F / K299E or T68L / T75W / K299E. The invention further discloses a preparation method of the urate oxidase mutant. According to the method, the key sites influencing the thermal stability are mined in the modes of directed evolution, high-throughput screening, multiple virtual screening and the like, and the stability is improved through mutation; the key sites influencing the enzymolysis resistance are excavated through molecular dynamics simulation and solvent accessible surface area calculation, and the enzymolysis resistance effect is improved through mutation. Finally, a plurality of key sites and mutants influencing the stability and the enzymolysis resistance are excavated, and the thermal stability and the enzymolysis resistance are improved.

The invention discloses uric acid-reducing peptide goat milk powder and a preparation method thereof, and belongs to the technical field of enzymes. According to the process for preparing the uric acid lowering peptide by hydrolyzing goat milk through the compound enzyme, the total addition amount of the compound enzyme (the enzyme activity ratio of neutral protease N to neutral protease X is 1: 1) is 4000 U / g-6000 U / g, enzymolysis is conducted for 2-3 h at the temperature of 45-55 DEG C, and under the condition, the xanthine oxidase inhibition rate is 71.67 + / -1.32%-83.64 + / -0.98%. The enzymolysis goat milk containing the uric acid-reducing peptide is subjected to spray drying to prepare the uric acid-reducing peptide goat milk powder, and the storage stability of the goat milk powder is relatively good. The amino acid sequence and the source of the uric acid lowering peptide are separated and identified, the in-vitro uric acid lowering activity of the uric acid lowering peptide is verified through the solid-phase synthesized uric acid lowering peptide, and a theoretical basis and technical support are provided for development of new uric acid lowering peptide goat milk products.

The invention discloses a urate oxidase mutant (rAfUOX) with trypsin and chymotrypsin tolerance and application of the urate oxidase mutant (rAfUOX). The invention provides a urate oxidase mutant with trypsin and chymotrypsin tolerance by modifying a key amino acid residue in a wild type aspergillus flavus urate oxidase molecule through a protein engineering technology. The activity half-life period of the recombinant aspergillus flavus urate oxidase mutant rAfUOXK49Q after trypsin digestion is increased by 244% compared with the activity half-life period of the wild type rAfUOX, and the activity half-life period of the recombinant aspergillus flavus urate oxidase mutant rAfUOXK49Q after chymotrypsin digestion is increased by 191% compared with the activity half-life period of the wild type rAfUOX. Other enzymatic properties are basically consistent with those of the wild type rAfUOX.

The application discloses a tetrapeptide IR4 with uric acid reducing activity, and a preparation method and application thereof, and belongs to the technical field of small molecular peptides. The amino acid sequence of the tetrapeptide IR4 is IDWR, and the tetrapeptide IR4 can be prepared by a solid-phase synthesis method and an enzymatic hydrolysis method. The tetrapeptide IR4 is identified from a porphyra haitanensis protease hydrolysate, has potential interaction with xanthine oxidase, and can reduce the uric acid content of zebrafish by 33.6% (to 5.93+ / -0.47 mu mol / g protein) at a concentration of 100 mu g / mL. Compared with an anserine (which can reduce the uric acid content of zebrafish by 25.7%), the tetrapeptide IR4 has better uric acid reducing activity. Compared with allopurinol (which can reduce the uric acid content of zebrafish to 4.61+ / -1.11 mu mol / g protein), the uric acid reducing ability of the tetrapeptide IR4 and the allopurinol has no significant difference. The tetrapeptide IR4 can be used for preparing uric acid reducing drugs and relieving hyperuricemia.

The invention belongs to the technical field of biology, and particularly relates to regulation and control of cinnamyl aldehyde on hyperuricemia related gene promoter region G quadruplex. According to the application disclosed by the invention, firstly, the capacity of cinnamyl aldehyde for regulating and controlling a G quadruplex in a hyperuricemia related gene promoter region is explored by utilizing circular dichroism, and the influence of cinnamyl aldehyde on regulation and control of a nucleic acid structure is further evaluated by utilizing a fluorospectro photometer, so that support is provided for a molecular mechanism of cinnamyl aldehyde for relieving hyperuricemia on a gene level. In conclusion, the invention discloses a brand new mechanism that cinnamyl aldehyde regulates and controls uric acid metabolism related genes through a targeted G quadruplex structure, a precise action target and a potential drug development direction are provided for treatment of hyperuricemia, and meanwhile, an established small molecule and G quadruplex interaction verification method provides technical reference for similar research.

The invention relates to the technical field of preparation of deep-sea longevity fish precise active peptides, in particular to a deep-sea longevity fish precise active peptide with uric acid reducing and anti-inflammatory effects as well as a preparation method and application of the deep-sea longevity fish precise active peptide. The preparation method comprises the following steps: carrying out medium-temperature pretreatment, carrying out step-by-step enzymolysis with alkaline protease and acid protease, centrifuging, filtering, carrying out ultrafiltration interception and the like, and screening out 10 non-reported non-toxic active peptide fragments. The active peptide has a multi-target synergistic effect, inhibits the activity of xanthine oxidase, regulates and controls the expression of uric acid resorption protein, regulates and controls the expression of uric acid transporter protein, realizes bidirectional regulation of uric acid metabolism, efficiently removes hydroxyl radicals, activates superoxide dismutase and reduces the content of inflammation-related factors. The technology is simple and easy to control, low in equipment requirement and suitable for large-scale production, the active ingredients are natural, safety is high, the active ingredients can serve as functional factors to be applied to functional food, health care products and the like, and safe and diversified intervention schemes are provided for hyperuricemia and related inflammation problems.

The invention discloses a method for promoting hermetia illucens to convert chicken manure by utilizing uric acid degrading bacteria, belongs to the technical field of microorganisms, and solves the problems that in the prior art, when the hermetia illucens treats the chicken manure, the hermetia illucens grow slowly due to too high uric acid content in the chicken manure, and the hermetia illucens sand needs to be fermented again. The invention relates to a method for promoting hermetia illucens to convert chicken manure by using uric acid degrading bacteria, which comprises the following steps: inoculating the uric acid degrading bacteria into an LB broth liquid culture medium, culturing overnight on a horizontal shaking table under the conditions of 37 DEG C and 180 rpm, centrifugally concentrating strain fermentation liquor to obtain a strain working solution, adding the strain working solution into the chicken manure, and treating the chicken manure by using hermetia illucens larvae which are fattened for 1-5 days. When the hermetia illucens treats the chicken manure, uric acid in the chicken manure can be remarkably reduced, growth and development of the hermetia illucens can be promoted, the process steps can be shortened, the problem that the hermetia illucens need to be fermented again is solved, and the production efficiency is improved.

The invention belongs to the technical field of biological fermentation, and particularly relates to a preparation method and application of a fermented rhizoma smilacis glabrae extract. The invention relates to a fermented rhizoma smilacis glabrae extract, which is characterized in that a lactobacillus rhamnosus LR.M8 strain is utilized, the inhibition effect of the rhizoma smilacis glabrae extract on xanthine oxidase is improved through fermentation, and the treatment effect of the rhizoma smilacis glabrae extract on hyperuricemia is improved. The solution for improving the effect of the extract through biological fermentation is provided for solving the problems that the content of effective components in common medicinal and edible extracts is low, and the human body utilization rate is not high. The lactobacillus rhamnosus LR.M8 can effectively improve the adjusting effect of the rhizoma smilacis glabrae medicine and food homologous plant extract on uric acid balance, can reduce damage of inflammation to cells, and promotes in-vivo purine metabolism to recover balance. The fermentation method provided by the invention is simple and remarkable in effect, and has a wide application prospect.

The application discloses a pentapeptide IK5 with uric acid reducing activity as well as a preparation method and application thereof, and belongs to the technical field of small molecular peptides. The amino acid sequence of the pentapeptide IK5 is ITGYK, and the pentapeptide IK5 can be prepared by a solid-phase synthesis method and an enzymolysis method. The pentapeptide IK5 is identified from a protease enzymolysis liquid of a fine weak red winged seaweed, has potential interaction with xanthine oxidase, and can reduce the uric acid content of zebrafish by 44.4% (to 5.93+ / -0.47 mu mol / g protein) at a concentration of 100 mu g / mL. Compared with ananeine (which can reduce the uric acid content of zebrafish by 25.7%), the pentapeptide IK5 has better uric acid reducing activity. Compared with allopurinol (which can reduce the uric acid content of zebrafish to 4.61+ / -1.11 mu mol / g protein), the uric acid reducing ability of the pentapeptide IK5 and the allopurinol has no significant difference. The pentapeptide IK5 can be used for preparing uric acid reducing drugs and relieving hyperuricemia.

The invention relates to a food uric acid reducing composition. The food uric acid reducing composition comprises main materials and auxiliary materials, the mass ratio of the main material to the auxiliary material is 5: 1; the main material is prepared from the following raw materials in parts by weight: 10 to 15 parts of frankincense powder, 10 to 15 parts of tuna peptide, 50 to 100 parts of freeze-dried mulberry powder, 50 to 100 parts of soy isoflavone, 30 to 50 parts of red yeast rice powder, 10 to 20 parts of turmeric powder, 40 to 80 parts of marine red algae powder and 100 to 200 parts of celery seed powder; the auxiliary materials comprise at least one of the following auxiliary materials: a first auxiliary material which is selected from at least one of chicory powder, poria cocos powder and B vitamins; the second auxiliary material is selected from at least one of radix puerariae powder and lophatherum gracile powder; and the third auxiliary material is selected from at least one of sour cherry powder and licorice powder. According to the food uric acid reducing composition, the uric acid reducing effect is improved through combination of specific raw materials, the taste and taste of the product can be improved through food-borne materials, side effects are reduced, and the food uric acid reducing composition is suitable for being taken for a long time.

The invention provides a kiwi flower extract solid beverage with a uric acid reducing effect as well as a preparation method and application thereof, and belongs to the technical field of functional foods. Fresh yellow heart kiwi fruit flowers are sequentially subjected to drying, smashing, microwave enzyme deactivation and vacuum freeze drying operation to obtain kiwi fruit flower powder, then the kiwi fruit flower powder is subjected to gradient targeted extraction, firstly, subcritical water extraction is adopted, the flavone yield is increased, macromolecular impurities are removed through ceramic membrane filtration, an extracting solution A and filter residues are obtained, and the yellow heart kiwi fruit flower powder is obtained. The preparation method comprises the following steps: mixing filter residues with ethanol, then carrying out ultrasonic extraction to improve the extraction rate of polyphenol in the extract, carrying out suction filtration and concentration to obtain an extracting solution B, finally carrying out steady-state processing, mixing the extracting solution A and the extracting solution B, and carrying out freezing granulation to obtain the kiwi flower extract solid beverage with the effect of reducing uric acid and high content of flavonoid and high-phenol effective substances. The solid beverage of the kiwi flower extract has an excellent improvement effect on hyperuricemia.

The invention discloses a breeding method for intensively preventing and controlling goose gout and application of the breeding method, and aims to solve the technical problems of high incidence of goose gout and low survival rate in intensive goose breeding. According to the method, prevention and control are achieved through a cooperative scheme of graded disinfection, precise trace element supplement and environment regulation and control; 50-250 ppm hypochlorous acid is adopted for conducting graded sterilization on the goose house environment, a feeding trough and drinking water, and pathogen breeding and urate deposition inducements are cut off; when the geese are 15-75 days old, a German Q life water trace element solution is added into drinking water, the excretion function of the kidney is enhanced, and metabolic balance is adjusted; meanwhile, the breeding density is controlled to be 1-4 / m, the environment humidity is 55-70%, and ventilation and daily average activity amount are guaranteed. The method is accurate and safe in prevention and control and free of drug residues, the gout incidence rate of goose groups can be reduced to 4% or below, the survival rate is increased to 95% or above, the weight gain efficiency in the breeding period is increased by 10%-15%, the breeding cost and loss are remarkably reduced, the method is suitable for intensive breeding scenes of medium and small meat geese, breeding geese and the like, and comprehensive economic benefits are outstanding.

The invention relates to the field of bioelectrochemical sensors, in particular to an epitaxial gate type FET uric acid sensor and a uric acid concentration detection method. The FET uric acid sensor based on the epitaxial gate type comprises a field effect transistor and an epitaxial gate, the epitaxial grid electrode comprises ITO (Indium Tin Oxide) conductive glass connected with the grid electrode of the field effect transistor, an MXene / Ppy composite modification layer, a polypyrrole layer and a solution tank; the MXene / Ppy composite modification layer and the polypyrrole layer are sequentially deposited on the upper surface of the ITO conductive glass from bottom to top; the polypyrrole layer is provided with a specific imprinting cavity of uric acid to be detected; and the solution tank is arranged on the upper surface of the polypyrrole layer and is used for accommodating detection liquid. According to the invention, rapid, sensitive, accurate and low-cost detection of uric acid molecules in a low-concentration range can be realized.

This invention provides a traditional Chinese medicine composition for treating renal edema, pyelonephritis with ascites, and nephritis. It consists of over 20 herbs including Atractylodes lancea, Areca catechu, Saposhnikovia divaricata, and Alisma plantago-aquatica (specific formulation details can be found in the original text). It can be prepared as pills, granules, or decoctions for oral administration. Core efficacy: 1. Promotes diuresis and reduces swelling, eliminating renal ascites; 2. Clears heat and detoxifies, anti-inflammatory and antibacterial (synergistic effect on fungal infections and cervical erosion); 3. Regulates renal function and improves abnormal uric acid metabolism. Treatment plan: Mild cases: Use five basic formulas (①-⑤) composed of Codonopsis pilosula, Poria cocos, etc., taken three times daily; Severe cases: Use a potent diuretic formula containing Akebia trifoliata and Euphorbia pekinensis (one spoonful of pills twice daily); Treatment course is approximately one month, with a follow-up examination after three months. Advantages: Cure rate reaches 90%, low recurrence rate – pure traditional Chinese medicine preparation, no significant toxic side effects; also treats complications (fungal infection, cervical lesions). This treatment plan has been clinically verified through three generations and has significant efficacy in treating renal edema, ascites, and nephritis.

The invention belongs to the technical field of biological medicine, and particularly discloses a tea polyphenol self-polymerization nanoparticle for intracellular delivery of uricase and a preparation method and application thereof.The tea polyphenol self-polymerization nanoparticle comprises a tea polyphenol self-polymerization compound and uricase, the tea polyphenol self-polymerization compound is formed by preliminary self-polymerization of epigallocatechin gallate under catalysis of Mn < 2 + >, and the uricase is prepared from the tea polyphenol self-polymerization compound and the uricase; and mixing the polyphenol self-polymerized compound with uricase, and entrapping the uricase to obtain the tea polyphenol self-polymerized nanoparticles. The tea polyphenol self-polymerization nanoparticles have the functions of uricase combined delivery and synergistic treatment based on EGCG (epigallocatechin gallate) carriers (TPNs), the tertiary structure integrity of uricase can be effectively maintained, and the activity of uricase is ensured; meanwhile, uricase can be protected from being damaged by protease, and the stability of uricase is enhanced. Through the synergistic effect of uricase and TPNs in cells, both symptoms and root causes of intracellular urate crystallization and inflammatory reaction are treated, and a better scheme choice is provided for gout or hyperuricemia.

The invention relates to the technical field of protein drugs, in particular to a urate oxidase variant and a glycosylation modification form thereof. An engineered N-glycosylation site is introduced into urate oxidase, and a pair of engineered disulfide bonds is additionally introduced into a tetramer interface, so that the obtained glycosylation modified urate oxidase variant can improve folding stability, in-vivo performance and immunological properties on the basis of maintaining enzymatic activity.

This invention discloses a multi-channel microfluidic chip and device for detecting creatinine and uric acid. The invention is based on the principle that the H2O2 produced by the reaction of creatinine and creatinine hydrolase, and the H2O2 produced by the reaction of uric acid and uricase, rapidly quench the fluorescence of the aerogel. Sensitive detection of urinary creatinine and uric acid is achieved by detecting the fluorescence intensity. Because the sensor uses paper-based fiber material as a substrate and introduces quantum dot nanomaterials as sensitive units to construct a three-dimensional aerogel structure, it has characteristics such as a large specific surface area, providing more active sites for the adsorption of biological enzymes, thereby increasing the adsorption capacity. Simultaneously, the presence of a large amount of glutathione on the surface of the quantum dots constituting the aerogel provides good biocompatibility, which is beneficial for maintaining enzyme activity, thus improving the sensitivity and stability of the detection.

The invention relates to application of a dry memory T cell in preparation of a medicine for treating colorectal cancer pulmonary metastasis, and belongs to the technical field of biological medicine. The invention firstly proves that the capability of differentiating the Tscm cells into effector killer T cells can be effectively improved by knocking out the Erbin genes in the Tscm cells in a mouse body, lung metastasis of colorectal cancer is inhibited, and the treatment effect of CAR-T cells can be enhanced by the Tscm cells with the Erbin genes knocked out. In addition, xanthine is detected for many times in a mouse colorectal cancer lung metastasis focus after the Erbin gene in the Tscm cell is knocked out, hypoxanthine can promote generation of more Tscm cells in the tumor metastasis focus, and the Tscm cells are differentiated to generate killer T cells, so that colorectal cancer lung metastasis is inhibited. The metabolic pathway of hypoxanthine is further studied, it is found that allopurinol can inhibit conversion of hypoxanthine into xanthine and uric acid, and it is found that allopurinol can also inhibit colorectal cancer lung metastasis by injecting allopurinol into a colorectal cancer lung metastasis mouse model.

The utility model provides fermentation equipment for a radix puerariae beverage capable of reducing uric acid, which belongs to the field of fermentation equipment, and comprises a fermentation container and a sealing cover at the top of the fermentation container, and a stirring ball is arranged in the fermentation container; an interlayer structure is arranged at the inner bottom of the fermentation container and is used for bearing solute raw materials of the kudzuvine root beverage in a layered manner. According to the utility model, a stirring shaft in the prior art is replaced by the stirring ball, so that the occupied effective contact area between a compound raw material containing radix puerariae and sterile water is reduced, and the compound raw material containing radix puerariae is ensured to be fully and uniformly contacted with strains; an interlayer structure is arranged at the bottom in the fermentation container, so that the solute raw materials of the kudzuvine root beverage are prevented from being completely stacked together, and further, the contact between the solute raw materials of the kudzuvine root beverage and sterile water containing strains under the non-stirring condition is increased in a mode of'integrating into scattering '.

The invention discloses a nucleic acid molecule for coding urate oxidase, a related product and an application of the nucleic acid molecule. The invention discloses a nucleic acid molecule for coding urate oxidase. The nucleic acid molecule comprises a nucleotide sequence as shown in SEQ ID NO. 2. According to the invention, a microbe-derived urate oxidase gene sequence for escherichia coli expression is obtained for the first time, and the gene sequence is easy to clone and express and good in repeatability. The invention further discloses a recombinant vector and a bioengineering bacterium for urate oxidase expression, and the obtained urate oxidase protein is high in activity and simple to prepare.

The invention belongs to the technical field of gene engineering, and particularly relates to a 3-ketosteroid-delta1-dehydrogenase mutant with improved heat resistance and an application of the 3-ketosteroid-delta1-dehydrogenase mutant. The 3-ketosterone-delta1-dehydrogenase AuKsdD gene derived from arthrobacter urate oxydans is subjected to structural analysis and site-directed mutagenesis design, and a mutant with higher catalytic activity and remarkably enhanced heat resistance compared with a wild type AuKsdD is obtained through directed mutation of key sites E244, K267, T301 and A335. The mutant can keep high catalytic efficiency for a long time under a high-temperature condition, and is suitable for industrial synthesis of steroid drug raw materials.

The invention discloses a stable lactic dehydrogenase and uric acid combined detection kit with strong anti-interference capability, and belongs to the technical field of detection. The joint detection kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 contains a buffer solution, a composite anti-interference agent, a composite stabilizer, L-lithium lactate, a compound surfactant, 4-aminoantipyrine, peroxidase and a preservative diazolidinyl urea; the reagent R2 contains a buffer solution, a coenzyme (NAD < + >), N-ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt uricase, a compound stabilizer, a compound surfactant and a preservative epsilon-polylysine hydrochloride. According to the present invention, the lactic dehydrogenase detection and the uric acid detection can be simultaneously achieved by one reagent, such that the use convenience is substantially improved, and the stability and the anti-interference ability of the reagent are further improved. The reagent disclosed by the invention is simple, convenient and rapid to operate, is suitable for automatic analysis, and is a lactic dehydrogenase and uric acid combined detection reagent which is more stable and strong in anti-interference capability.

The invention provides a method for screening a urate transporter inhibitor in vitro, and belongs to the technical field of biological detection. The invention constructs a cell model for expressing urate transporter, and provides a method for screening urate transporter inhibitors by a fluorescence detection method based on a microplate reader. According to the method, the screening efficiency is remarkably improved, an effective platform is provided, the method is used for systematically evaluating the inhibition effect of environmental pollutants on the urate transporter, the method has the advantages of being high in sensitivity and selectivity, easy and convenient to operate, safe and environmentally friendly, and more choices are provided for screening urate transporter inhibitors.

A strain of Bacteroides xylanisolvens capable of preventing gout in goslings and its medical use are provided, and the application of the strain in livestock and poultry breeding is also provided. The Bacteroides xylanisolvens was deposited at the General Microbiological Center of the China Committee for Culture Collection of Microorganisms on March 28, 2023, and named Bacteroides ( Bacteroides sp ), with the deposit number of CGMCC No. 26995. This strain can improve the growth performance of goslings, reduce the serum uric acid value of goslings, has no harmful effects on the liver and kidney functions of goslings, can enhance the expression of uric acid excretion-related enzymes and reduce the expression of uric acid production-related enzymes, can prevent gout in goslings, has the potential to improve the economic benefits of farmers, and has a wide application prospect.

The invention belongs to the technical field of biological medicine, and particularly relates to Ce-MOF (at) Pt nano-enzyme as well as a preparation method and application thereof. The Ce-MOF (at) Pt nano-enzyme is composed of Ce-MOF and Pt nano-enzyme, and the preparation method comprises the following steps: adding a Ce (NO3) 3 solution into an H3BTC solution for reaction to obtain a product A; the obtained product A reacts with a mixed solution of NaOH and H2O2, and Ce-MOF is obtained; and reacting the obtained Ce-MOF with H2PtCl6, transferring the reaction solution to an ice-water bath, adding NaBH4, and continuing the reaction to obtain the Ce-MOF (at) Pt nano-enzyme. The Ce-MOF (at) Pt nano-enzyme has high penetrability, urate oxidase activity and catalase activity, and can assist arthroscopic surgery to remove tophus, reduce iatrogenic injury and inhibit inflammatory response caused by MSU. The invention provides a brand-new strategy for the treatment of gouty arthritis.