62 results about "Uricotelic" patented technology

The present invention provides a composition comprising at least one microorganism selected from the group consisting of lactic acid bacteria and yeasts that have an ability to decompose purines and an action of lowering serum uric acid level and in particular, such a composition in a food, beverage or pharmaceutical form. Such a composition is effective in the prevention and amelioration of hyperuricemia.

The invention discloses a metabonomics-based chilled fresh meat freshness marker and a screening and prediction model fitting method and application thereof. The marker is prepared from indole-3-formaldehyde, uridine monophosphate, phenylmercaptouric acid, gluconic acid, tyramine and serine-phenylalanine. The prediction model is: Y=3.964+1.97E<-7>X1- 4.22E<-7>X2-3.37E<-7>X3+8.80E<-8>X4+1.26E<-8>X5-5.57E<-7>X6. According to the method, an Agilent 1290 UHPLC is connected with a Q Exactive Orbitrap high-resolution mass spectrum in series, the method has higher resolution, more substances can be detected more accurately, metabolites in the preservation process of the chilled fresh chicken can be illustrated more comprehensively, and the obtained result is more reliable.

The invention relates to collagen peptide lubricating fluid and a preparation method thereof. The collagen peptide lubricating fluid is prepared from the following raw materials in parts by weight: 5-20 parts of tea fungus fermentation liquor, 0.5-1.2 parts of hyaluronic acid, 1-2 parts of tea fungus fermentation membrane modifiers, 8-12 parts of collagen peptides, 0.3-0.5 part of cananga odorataessential oil, 3-5 parts of water soluble camellia oleosa seed oil, 0.5-1 part of hydroxyethyl urea, 0.5-1 part of hexanediol and 60-80 parts of deionized water. The collagen peptide lubricating fluidis prepared from natural plants, is carried and used conveniently and has the effects of effectively cleaning and improving sexual excitation, and therefore, the collagen peptide lubricating fluid isa lubricating product which can be used for a long time.

The invention discloses a kit and a method for simultaneously detecting tryptophan and metabolites thereof in a biological sample based on ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), which belong to the technical field of UPLC-MS/MS detection. The kit comprises standard substances of tryptophan and metabolites of the tryptophan, and the tryptophan and the metabolites of the tryptophan comprise 3-hydroxyl kynurenine, 5-hydroxytryptamine, kynurenine, yellow uric acid, tryptophan, kynurenic acid, 3-hydroxyl o-aminobenzoic acid, 5-hydroxyl indoleacetic acid, 3-indoleacetic acid and 3-indolepropionic acid. According to the method, the tryptophan and the metabolites of the tryptophan are detected by utilizing UPLC-MS/MS, and a standard curve is established according to a peak area, so that the contents of the tryptophan and the metabolites of the tryptophan are obtained. The method for detecting the tryptophan and the metabolites of the tryptophan is simple to operate, has the characteristics of high sensitivity, good selectivity and the like, can accurately and quickly detect the tryptophan and the metabolites of the tryptophan at the same time, and has wide application value.

The invention relates to a urea granule dense-phase conveying method and system. The urea granule dense-phase conveying system comprises a dense-phase conveying air source control unit, a rotating discharge valve unit, a dense-phase elutriator and a tail gas treatment unit. The granule dense-phase conveying method comprises the following steps: urea granular materials are fed by a urea granule hopper into a dense-phase conveying pipeline through a rotating discharge valve, a plug and delivery air in the dense-phase conveying pipeline tangentially enter a feed cylinder of the dense-phase elutriator to form continuous materials and air at the upper part of the feed cylinder, the material flow is accelerated through an accelerating tube, the granular material discharged from the accelerating tube is separated from the air flow due to inertia, the separation of the granular material from the powdered material is realized, the granular material enters a collecting hopper and is fed into a downstream storage bin through a gravity rotary valve; the powdered material enters an exhaust gas pipeline to be entrained from the hopper along the reverse direction of air flow; powdered material-entrained delivery tail gas enters a bag filter, the powdered material is captured, and the gas is discharged after being filtered. According to the urea granule dense-phase conveying method and system, the concept of the dense-phase elutriator is put forward for the first time, after the plug in the pipeline passes through the system, continuous separation of the uric acid powered material from granular material under the dense-phase condition can be realized.

The invention relates to a portulaca oleracea active component and a preparation method and application thereof, and belongs to the technical field of natural active components. The preparation methodof the portulaca oleracea active component comprises the following steps of performing extraction: taking portulaca oleracea, adding water as a solvent, and performing heating extraction to obtain awater extract solution; and performing ultrafiltration: filtering the water extract solution through an ultrafiltration membrane with the molecular weight cutoff of 5K-15K, and taking a filtrate, so that the portulaca oleracea active component is obtained. The preparation method of the portulaca oleracea active component adopts an ultrafiltration mode for extraction and purification and has the advantages of simple and feasible process, experiments prove that the obtained portulaca oleracea active component has good anti-inflammatory and analgesic effects, and biochemical index detection and pathological analysis of animal experiments prove that the component has a good urate-lowering effect and a good kidney protecting effect. Furthermore, the therapeutic effect of PO on gouty diseases isexplored, and a basic theory and a scientific basis are provided for subsequent research and patent medicines of the PO.

The invention provides a method for detecting the content of urate oxidase in serum, which is characterized by comprising the following steps: 1) preparation of a standard substance: taking urate oxidase with known enzymatic specific activity, and diluting with a Tris-HCl solution; 2) standard curve making: adding a working solution into a test tube, adding a standard substance and blank serum, adding a stop solution to stop the reaction after constant-temperature water bath reaction, and detecting a light absorption value; 3) sample detection: adding a working solution into the test tube, then adding a to-be-detected sample, adding a stop solution to stop the reaction after the constant-temperature water bath reaction, and detecting a light absorption value; 4) data determination and calculation: calculating the enzyme activity of the standard substance at each point according to the enzyme specific activity of the standard substance and the protein content of the standard substance at each point of the standard curve; fitting a standard curve for the enzyme activity of the standard substance at each point according to the light absorption value of each point of the standard curve; substituting the light absorption value of the to-be-detected sample into the standard curve, and obtaining the protein content of the to-be-detected sample through a calculation formula. The methodis accurate, simple and rapid, and the cost is greatly saved compared with an existing complex method.

The invention relates to a preparation method and applications of a flexible and high-selectivity non-enzymatic uric acid electrode carbon fiber membrane of self-supporting carbon fiber, wherein the flexible and high-selectivity non-enzyme uric acid electrode carbon fiber membrane of self-supporting carbon fibers is prepared and is used as an electrochemical sensor modification electrode to directly and rapidly determine non-enzymatic uric acid. The method comprises the following steps: dissolving PAN in DMF to prepare an electrospinning precursor with a PAN mass concentration of 10-14%, applying a voltage between a needle and an aluminum foil collector, carrying out electrospinning, carrying out vacuum drying on the prepared polymer nanofiber membrane to volatilize DMF, putting the carbonfiber membrane into a quartz tube furnace, carrying out heat treatment, cooling to a room temperature, fixing the obtained carbon fiber membrane by using a platinum electrode clamp, exposing, immersing into an H2SO4 solution, and activating by using a cyclic voltammetry to obtain a flexible carbon fiber membrane. With the application of the flexible and high-selectivity non-enzymatic uric acid electrode carbon fiber membrane as a non-enzyme UA sensor electrode, the electrode has characteristics of rapid determination, sensitivity, accuracy, stability, environmental protection and the like, and is an innovation in non-enzyme UA sensor measurement.

The invention relates to application of gentiopicroside in preparation of medicines for treating non-alcoholic fatty liver diseases. Experiments prove that the gentiopicroside can achieve the following effects in non-alcoholic fatty liver diseases caused by fructose: obviously limiting increase of mouse weight and decrease of food ration, lowering triglycerides (TG), uric acid (UA) and glucose (GLU) as well as levels of serum glutamic oxalacetic transaminase (AST) and glutamic-pyruvic transaminase (ALT), obviously alleviating vacuolar degeneration of hepatic cells and deposition of fatty acidscaused by fructose, lowering levels of tumor necrosis factors (TNF-alpha) and interleukin 6 (IL-6) in hepatic tissues, raising levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px)in the hepatic tissues and lowering levels of malondialdehyde (MDA) and superoxide anions (O2<->). Results show that the gentiopicroside can achieve the effects of obviously promoting metabolism of the liver on fat, obviously improving and increasing the antioxidant stress level of the liver and alleviating hepatocellular injury, so that the aim of treating the fatty liver diseases is achieved.

The invention discloses a formula of a tablet candy with an auxiliary uric acid reducing function. The formula comprises the following raw materials in parts by weight: 80-220 parts of sorbitol, 85-255 parts of marine fish oligopeptide powder (containing goose carnosine), 175-525 parts of acerola cherry powder, 15-45 parts of turmeric, 40-110 parts of chitosan oligosaccharide, 5-20 parts of a grape seed extract, 42-125 parts of poria cocos powder, 80-180 parts of microcrystalline cellulose and 2-7 parts of magnesium stearate. Most of existing medicines (such as allopurinol, benzbromarone and febuxostat) for reducing uric acid have side effects and can cause harm to human bodies after being used for a long time. The tablet candy adopts natural and healthy animal and plant components, and the marine fish oligopeptide powder containing goose carnosine, turmeric, chitosan oligosaccharide, the grape seed extract and poria cocos powder are combined for use to achieve the effect of reducing uric acid, so that the safety is high; and through a reasonable formula proportion, the product achieves a good taste, and meanwhile, on the premise of ensuring the effects, consumers can enjoy the tablet candy better and the compliance is high.

The invention provides a preparation method of a titanium nitride nanowire/nanotube array integrated electrode capable of simultaneously detecting dopamine, uric acid and ascorbic acid. The preparation method comprises the following steps: packaging the side surface and the back surface of a pretreated titanium sheet; anodizing the packaged titanium sheet; cleaning and drying the titanium sheet subjected to anodic oxidation; annealing the cleaned and dried titanium sheet to form anatase TiO2 on the surface of the titanium sheet; and carrying out heat treatment on the annealed titanium sheet inan NH3 atmosphere to obtain the titanium nitride nanowire/nanotube array integrated electrode. According to the preparation method of the titanium nitride nanowire/nanotube array integrated electrodefor simultaneously detecting dopamine, uric acid and ascorbic acid provided by the invention, materials are easy to obtain, the process is simple and controllable, lower detection limits can be respectively detected for dopamine and uric acid, and dopamine, uric acid and ascorbic acid can be detected at the same time, so that the good resolution capability is shown.

The invention relates to the technical field of medicine and food, in particular to a composition with a uric acid reducing function and sacha inchi polypeptide biscuits. The composition with the uric acid reducing function comprises sacha inchi polypeptide, oat fibers, poria cocos powder, psyllium husk powder, hericium erinaceus powder, phaseolus calcaratus powder and coix seed powder; the sacha inchi polypeptide is used as a main raw material for reducing uric acid, and the oat fibers, the poria cocos powder, the psyllium husk powder, the hericium erinaceus powder, the phaseolus calcaratus powder and the coix seed powder are combined, and the components can achieve a synergistic effect, enhance the uric acid reducing effect and promote uric acid excretion. The composition raw materials adopted by the sacha inchi polypeptide biscuit are food-grade raw materials and can be eaten for a long time, the sacha inchi polypeptide is used as a main acting raw material, and a reference basis is provided for application of the sacha inchi polypeptide in the field of functional foods. The preparation method of the sacha inchi polypeptide biscuit is simple to operate and easy to popularize, and has a good market application prospect.

The invention discloses a nanogold cluster preparation method and a biological small molecule sensitization detection method, uric acid generates hydrogen peroxide under the catalysis of urate oxidase, a substrate 3, 3 ', 5, 5'-tetramethyl benzidine is oxidized into ox-TMB by using peroxidase activity of the synthesized gold nanocluster and a catalytic product H2O2, at the moment, an emission spectrum of AuNCs is overlapped with an absorption spectrum of ox-TMB, and the concentration of the ox-TMB is determined. According to the method disclosed by the invention, fluorescence resonance energy transfer occurs between the strongly fluorescent AuNCs and ox-TMB, a BSA-AuNCs fluorescence signal achieves a quenching effect, and the method not only solves the problem that use conditions of a traditional enzyme method are restricted, but also reduces a detection line and realizes sensibilization detection of uric acid molecules. Due to the fact that the emission spectrum of the AuNCs is overlapped with the absorption spectrum of the ox-TMB, fluorescence resonance energy transfer occurs between the AuNCs with strong fluorescence and the ox-TMB, the fluorescence signal of the AuNCs is obviously reduced, and the purpose of sensitizing and detecting the content of the uric acid is achieved.

The invention relates to a hemp seed bioactive peptide for regulating an HPRT1 gene and an OAT1 protein and a preparation method of the hemp seed bioactive peptide, and belongs to the technical field of functional food development. In order to solve the problems that at present, the utilization rate of hemp seed processing byproducts is low, and most gout treatment drugs have serious side effects, the uric acid reducing action way of hemp seed bioactive peptides is researched from the molecular mechanism level, and the method comprises the steps that hemp seed protein powder is subjected to superfine grinding to be 100 meshes or above, water is added, and ultrahigh pressure homogenization is conducted; and carrying out enzymolysis (the temperature of 1% of excision aminopeptidase is 55 DEG C and the action time is 90 minutes), carrying out dynamic cross-flow concentration membrane filtration, collecting filtrate, and carrying out freeze drying. Physical ultrahigh pressure is combined with an excision protease enzyme method for hydrolysis to prepare the hemp seed bioactive peptide, and the hemp seed bioactive peptide capable of up-regulating the HPRT1 gene and the OAT1 protein is obtained by adopting a dynamic cross-flow concentration membrane filtration technology, so that the method is a safe and efficient way.

The invention discloses a nucleotide sequence and protein sequence of a novel OsXDH (oryza sativa xanthine dehydrogenase) gene and a function of the OsXDH gene in delaying leaf senescence through overexpression, and belong so the field of genetic engineering. The cDNA (complementary deoxyribonucleic acid) sequence of the OsXDH is shown in the SEQ ID NO.1 and a coded amino acid sequence is shown in the SEQ ID NO.2. The novel OsXDH gene plays an important role in a purine metabolism process and catalyzes xanthine and hypoxanthine to generate uric acid. MRNA (messenger ribonucleic acid) expression analysis shows that the gene shows constitutive expression, and overexpression of the gene can delay oryza sativa senescence. Transgenic experiments prove that the gene has the function of delaying oryza sativa senescence, and on the basis of the function of the OsXDH gene in delaying of oryza sativa senescence through overexpression, the gene can be used for genetic breeding of oryza sativa.