96 results about "Uricotelic" patented technology

The invention discloses application of lactobacillus rhamnosus HPT112 in preparation of a medicine for preventing and / or treating hyperuricemia, and belongs to the field of microorganisms, the lactobacillus rhamnosus HPT112 is preserved in the China Center for Type Culture Collection on November 20, 2023, and the preservation number is CCTCC NO: M20232273. Tests prove that the lactobacillus rhamnosus HPT112 has the function of regulating bile acid metabolism, so that the uric acid reducing effect is achieved, and the uric acid reducing effect equivalent to or even better than that of an existing hyperuricemia medicine can be achieved. Compared with existing hyperuricemia drugs, the lactobacillus rhamnosus HPT112 has the advantages that toxic and side effects are low, safety is high, a brand-new drug for preventing and / or treating hyperuricemia can be developed by the aid of the lactobacillus rhamnosus HPT112, and accordingly inflammatory reaction caused by hyperuricemia can be prevented and / or treated.

The present invention provides a manganese metal-organic framework material with oxidase properties, a preparation method, and an application thereof. The material is prepared by the following method: using 1,3,5-trimethic acid as an organic ligand, manganese acetate tetrahydrate as a manganese source, and a water-ethanol solution as a solvent, to obtain a manganese metal organic framework (MOF) precursor via a solvothermal synthesis technique; the manganese MOF precursor is post-synthetically modified using an oxidant, wherein the oxidant is a mixed solution of an aqueous alkali metal hydroxide solution and H2O2, and the ratio of the manganese MOF precursor to the alkali metal hydroxide is (400-600) mg:(0.5-2) mmol. By using specific types and amounts of manganese MOF precursors and oxidants, the resulting metal-organic framework nanomaterial has specific enzyme-like properties, can exert the function of uricase, and has optimal and high uricase activity under neutral pH conditions.

The invention discloses application of laccase as a catalyst in catalytic synthesis of allantoin. According to the invention, laccase from thermophilus is used as an enzyme catalyst, uric acid is oxidized, hydrolyzed and decarboxylated to synthesize allantoin, and efficient biosynthesis of allantoin is realized under mild conditions; wherein the amino acid sequence of the laccase derived from the thermophilic fungi is as shown in SEQ ID NO. 1. The invention provides an efficient, environment-friendly and low-cost biosynthesis approach for biosynthesis of allantoin, and has industrial application prospects in biosynthesis of allantoin.

The present application relates to the technical field of microbial fermentation, and particularly relates to a universal fermentation device, a yogurt production system and a method for producing a yogurt with reduced uric acid. The universal fermentation device comprises a fermentation tank, a sampling tube, a test tube, a sliding member, a pressure detection module and a processor. The pressure detection module is installed on the test tube. The sliding member is slidingly fitted on the test tube, and the sliding member is connected with a first driving rod. The sliding member is provided with a clearance opening, and the clearance opening is matched with a plugging block. The plugging block is connected with a test rod and a second driving rod. One end of the sampling tube is communicated with the fermentation tank, and the other end is communicated with the test tube. The processor is used for determining the density of the fermentation sample and the viscosity of the fermentation sample. The universal fermentation device can realize continuous monitoring of the fermentation system during the fermentation process, effectively avoids sample confusion, improves the timeliness of analysis, ensures that the conditions reflected by the sample are highly consistent with the original fermentation system, and is suitable for various fermentation scenes.

The invention discloses a uric acid reducing composition containing eucommia polysaccharide as well as a preparation method and application thereof, and relates to the technical field of biological medicines. The uric acid reducing composition prepared by the invention is prepared from the eucommia polysaccharide, the mulberry leaf extract and the clove extract, and the eucommia polysaccharide, the mulberry leaf extract and the clove extract are compounded for use, so that generation-excretion dual blocking can be formed aiming at different links of uric acid metabolism, and multi-target synergistic uric acid reduction is realized. Meanwhile, plant-derived components are adopted, so that the safety is higher, and adverse reactions can be effectively reduced. Besides, lactobacillus sake, lactobacillus plantarum and kluyveromyces marxianus are adopted as fermentation strains to prepare the mulberry leaf extract through fermentation, and the content of effective components in the mulberry leaf extract can be effectively increased, so that the uric acid reducing effect of the prepared composition is improved.

The invention relates to the field of lactobacillus paracasei, in particular to a preparation method and application of lactobacillus paracasei for improving hyperuricemia, and the preparation method comprises the following steps: activating a lactobacillus paracasei Jlus66 strain through an MRS liquid culture medium at 36-38 DEG C, then adding the activated Jlus66 strain into the MRS liquid culture medium, and culturing at 36-38 DEG C to obtain the lactobacillus paracasei for improving hyperuricemia. And then centrifuging and abandoning supernate to obtain thalli, and dissolving the thalli with normal saline until the concentration of a bacterial suspension is 1 * 10 < 9 > cfu / ml. The invention provides a method for regulating uric acid metabolism by regulating intestinal flora through the lactobacillus paracasei Jlus66 so as to improve hyperuricemia, side effects can be effectively avoided, and the method is a more natural and safer way.

The invention discloses siRNA (small interfering Ribonucleic Acid) of a targeted urate transporter 1 and application of the siRNA. According to the invention, siRNA drug design is carried out on the URAT1 gene to obtain siRNA composed of positive-sense strand RNA and antisense strand RNA, siRNA capable of effectively silencing the gene expression level of URAT1 is screened from the siRNA, and part of siRNA sequences even silence the gene expression level of URAT1 to about 20%. The invention further discloses the modified siRNA which is obtained by chemically modifying the siRNA sequence and can effectively silence the gene expression level of URAT1, and a recombinant vector containing the siRNA or the modified siRNA. The siRNA, the modified siRNA and the recombinant vector containing the siRNA or the modified siRNA provided by the invention have the application in the aspects of preparation of gene therapy medicines for treating hyperuricemia or gout and the like.

The invention belongs to the field of salmon processing by-product utilization, and particularly relates to a method for obtaining salmon processing by-product active peptide through fermentation and application of the salmon processing by-product active peptide. According to the invention, five uric acid lowering peptides with relatively high xanthine oxidase inhibitory activity are prepared by combining a microbial fermentation method with an ultrafiltration and gel chromatographic column technology, and the specific sequences are IGAPDMAFPR, FDKPVSPLL, FVDPDNFRV, GEADFMDYGR and GNGPDWAKDL. The raw material adopted by the invention is salmon processing waste (brown meat), the price is low, the utilization rate of resources is improved, and waste and environmental pollution are avoided. The method is simple in process operation, low in production cost and good in application prospect.

The invention relates to a preparation method of a purple sweet potato leaf water extract with a uric acid reducing effect, and belongs to the field of food processing. Comprising the following steps: carrying out superfine grinding on dried purple sweet potato leaves; the purple sweet potato leaf superfine powder is added into 4 g / L of 1-butyl-3-methylimidazolium tetrafluoroborate ionic liquid according to the solid-liquid ratio of 1: 10 (g: mL), meanwhile, high-voltage pulse treatment is conducted, the pulse frequency is 1000-1200 Hz, the pulse width is 30-40 microseconds, the flow velocity is 200-500 L / h, the electric field intensity is 30-50 kV / cm, the pulse treatment time is 70-350 microseconds, and extraction is conducted for 20 min at the temperature of 25 DEG C. Filtering an extracting solution, recovering liquid, and performing vacuum reduced-pressure concentration. The concentrated solution is poured into an extraction kettle of supercritical CO2 extraction equipment, the extraction temperature is 60 DEG C, the extraction time is 1 h, the extraction pressure is 40 Mpa, cyclic extraction is conducted three times, liquid is collected, and the purple sweet potato leaf water extract is obtained. According to the method, active substances such as chlorogenic acid in the raw materials are promoted to be dissolved out, green and pollution-free efficient operation is achieved in the whole process, and a new technical approach is provided for efficient and accurate utilization of purple sweet potato leaves for inhibiting uric acid.

The invention discloses screening and application of salt-resistant lactobacillus plantarum with multiple uric acid reducing functions. Samples are screened and collected in soil, sauced beans, fermented flour paste, fresh milk, pickled vegetables and the like, a strain obtained through screening is identified as Lactobacillus plantarum WQ47 and is named as Lactobacillus plantarum WQ47, the strain has high inosine and guanosine degradation capacity, the degradation rate is 99.9% or above, the degradation rate is 99.0% or above, and therefore the serum uric acid level can be effectively reduced. Besides, the strain WQ47 is cultured in an MRS lactic acid bacteria culture medium to a fermentation supernatant, a bacterial suspension and a cell-free extract in the middle and later periods of logarithmic growth, the xanthine oxidase inhibition rates are 44.9%, 33.4% and 62.8% respectively, uric acid generation can be effectively inhibited, purine generation can be reduced by degrading inosine and guanosine and inhibiting the activity of xanthine oxidase, and the strain WQ47 can be used for preparing a new strain for preventing and treating uric acid generation. The traditional Chinese medicines jointly achieve the effects of relieving and treating the hyperuricemia. Moreover, the strain WQ47 screened by the invention can well survive in an environment with high content of salt (NaCl) of 20% (mass fraction), can be widely applied to fermented food, pickled food, functional food and the like, and has a better application effect on preventing, relieving and treating hyperuricemia, gout and complications of the hyperuricemia and the gout.

The invention discloses a urate oxidase mutant and application thereof in improving the thermal stability of urate oxidase, and belongs to the technical field of biology. The urate oxidase mutant disclosed by the invention is T68L / T75S / E222D / K299E, T68L / T75S / K299E or T75S / K299E, and the urate oxidase mutant disclosed by the invention is T68L / T75S / K299E. According to the method, the key sites influencing the thermal stability are mined in the modes of directed evolution, high-throughput screening, multiple virtual screening and the like, and the stability is improved through mutation. And finally, a plurality of key sites and mutants influencing the stability are excavated, and the thermal stability is improved.

The invention discloses a urate oxidase mutant and application thereof in improving the heat stability of urate oxidase and the trypsin resistance, and belongs to the technical field of biology. The urate oxidase mutant disclosed by the invention is Y47F / T68L / T75W / D111G / K204F / F259I / K299E, T68L / T75W / K204F / K299E or T68L / T75W / K299E. The invention further discloses a preparation method of the urate oxidase mutant. According to the method, the key sites influencing the thermal stability are mined in the modes of directed evolution, high-throughput screening, multiple virtual screening and the like, and the stability is improved through mutation; the key sites influencing the enzymolysis resistance are excavated through molecular dynamics simulation and solvent accessible surface area calculation, and the enzymolysis resistance effect is improved through mutation. Finally, a plurality of key sites and mutants influencing the stability and the enzymolysis resistance are excavated, and the thermal stability and the enzymolysis resistance are improved.

The invention provides a CcUOX gene of Colletotrichum camelliae and an application thereof. The CcUOX gene is related to pathogenicity and purine metabolism, belongs to the technical field of microbial genetic engineering, comes from Colletotrichum camelliae, and its cDNA sequence consisting of 906 nucleotides is shown in SEQ ID NO:1; a protein encoded by the provided CcUOX gene has an amino acid sequence consisting of 301 amino acids as shown in SEQ ID NO:2; the CcUOX gene can be applied in the field of tea tree anthracnose resistance genetic engineering, and can be applied in the fields of food science and engineering; an engineering strain expressing the CcUOX gene can be used for the degradation, utilization and environmental restoration of caffeine waste, and can be applied to uric acid degradation; and CcUOX can be used as a gene target in the design and screening of fungicidal bacteria for Colletotrichum camelliae.

The invention discloses uric acid-reducing peptide goat milk powder and a preparation method thereof, and belongs to the technical field of enzymes. According to the process for preparing the uric acid lowering peptide by hydrolyzing goat milk through the compound enzyme, the total addition amount of the compound enzyme (the enzyme activity ratio of neutral protease N to neutral protease X is 1: 1) is 4000 U / g-6000 U / g, enzymolysis is conducted for 2-3 h at the temperature of 45-55 DEG C, and under the condition, the xanthine oxidase inhibition rate is 71.67 + / -1.32%-83.64 + / -0.98%. The enzymolysis goat milk containing the uric acid-reducing peptide is subjected to spray drying to prepare the uric acid-reducing peptide goat milk powder, and the storage stability of the goat milk powder is relatively good. The amino acid sequence and the source of the uric acid lowering peptide are separated and identified, the in-vitro uric acid lowering activity of the uric acid lowering peptide is verified through the solid-phase synthesized uric acid lowering peptide, and a theoretical basis and technical support are provided for development of new uric acid lowering peptide goat milk products.

The invention discloses a urate oxidase mutant (rAfUOX) with trypsin and chymotrypsin tolerance and application of the urate oxidase mutant (rAfUOX). The invention provides a urate oxidase mutant with trypsin and chymotrypsin tolerance by modifying a key amino acid residue in a wild type aspergillus flavus urate oxidase molecule through a protein engineering technology. The activity half-life period of the recombinant aspergillus flavus urate oxidase mutant rAfUOXK49Q after trypsin digestion is increased by 244% compared with the activity half-life period of the wild type rAfUOX, and the activity half-life period of the recombinant aspergillus flavus urate oxidase mutant rAfUOXK49Q after chymotrypsin digestion is increased by 191% compared with the activity half-life period of the wild type rAfUOX. Other enzymatic properties are basically consistent with those of the wild type rAfUOX.

The application discloses a tetrapeptide IR4 with uric acid reducing activity, and a preparation method and application thereof, and belongs to the technical field of small molecular peptides. The amino acid sequence of the tetrapeptide IR4 is IDWR, and the tetrapeptide IR4 can be prepared by a solid-phase synthesis method and an enzymatic hydrolysis method. The tetrapeptide IR4 is identified from a porphyra haitanensis protease hydrolysate, has potential interaction with xanthine oxidase, and can reduce the uric acid content of zebrafish by 33.6% (to 5.93+ / -0.47 mu mol / g protein) at a concentration of 100 mu g / mL. Compared with an anserine (which can reduce the uric acid content of zebrafish by 25.7%), the tetrapeptide IR4 has better uric acid reducing activity. Compared with allopurinol (which can reduce the uric acid content of zebrafish to 4.61+ / -1.11 mu mol / g protein), the uric acid reducing ability of the tetrapeptide IR4 and the allopurinol has no significant difference. The tetrapeptide IR4 can be used for preparing uric acid reducing drugs and relieving hyperuricemia.

The invention belongs to the technical field of biology, and particularly relates to regulation and control of cinnamyl aldehyde on hyperuricemia related gene promoter region G quadruplex. According to the application disclosed by the invention, firstly, the capacity of cinnamyl aldehyde for regulating and controlling a G quadruplex in a hyperuricemia related gene promoter region is explored by utilizing circular dichroism, and the influence of cinnamyl aldehyde on regulation and control of a nucleic acid structure is further evaluated by utilizing a fluorospectro photometer, so that support is provided for a molecular mechanism of cinnamyl aldehyde for relieving hyperuricemia on a gene level. In conclusion, the invention discloses a brand new mechanism that cinnamyl aldehyde regulates and controls uric acid metabolism related genes through a targeted G quadruplex structure, a precise action target and a potential drug development direction are provided for treatment of hyperuricemia, and meanwhile, an established small molecule and G quadruplex interaction verification method provides technical reference for similar research.

The invention relates to the technical field of preparation of deep-sea longevity fish precise active peptides, in particular to a deep-sea longevity fish precise active peptide with uric acid reducing and anti-inflammatory effects as well as a preparation method and application of the deep-sea longevity fish precise active peptide. The preparation method comprises the following steps: carrying out medium-temperature pretreatment, carrying out step-by-step enzymolysis with alkaline protease and acid protease, centrifuging, filtering, carrying out ultrafiltration interception and the like, and screening out 10 non-reported non-toxic active peptide fragments. The active peptide has a multi-target synergistic effect, inhibits the activity of xanthine oxidase, regulates and controls the expression of uric acid resorption protein, regulates and controls the expression of uric acid transporter protein, realizes bidirectional regulation of uric acid metabolism, efficiently removes hydroxyl radicals, activates superoxide dismutase and reduces the content of inflammation-related factors. The technology is simple and easy to control, low in equipment requirement and suitable for large-scale production, the active ingredients are natural, safety is high, the active ingredients can serve as functional factors to be applied to functional food, health care products and the like, and safe and diversified intervention schemes are provided for hyperuricemia and related inflammation problems.

The invention discloses a method for promoting hermetia illucens to convert chicken manure by utilizing uric acid degrading bacteria, belongs to the technical field of microorganisms, and solves the problems that in the prior art, when the hermetia illucens treats the chicken manure, the hermetia illucens grow slowly due to too high uric acid content in the chicken manure, and the hermetia illucens sand needs to be fermented again. The invention relates to a method for promoting hermetia illucens to convert chicken manure by using uric acid degrading bacteria, which comprises the following steps: inoculating the uric acid degrading bacteria into an LB broth liquid culture medium, culturing overnight on a horizontal shaking table under the conditions of 37 DEG C and 180 rpm, centrifugally concentrating strain fermentation liquor to obtain a strain working solution, adding the strain working solution into the chicken manure, and treating the chicken manure by using hermetia illucens larvae which are fattened for 1-5 days. When the hermetia illucens treats the chicken manure, uric acid in the chicken manure can be remarkably reduced, growth and development of the hermetia illucens can be promoted, the process steps can be shortened, the problem that the hermetia illucens need to be fermented again is solved, and the production efficiency is improved.

The invention belongs to the technical field of biological fermentation, and particularly relates to a preparation method and application of a fermented rhizoma smilacis glabrae extract. The invention relates to a fermented rhizoma smilacis glabrae extract, which is characterized in that a lactobacillus rhamnosus LR.M8 strain is utilized, the inhibition effect of the rhizoma smilacis glabrae extract on xanthine oxidase is improved through fermentation, and the treatment effect of the rhizoma smilacis glabrae extract on hyperuricemia is improved. The solution for improving the effect of the extract through biological fermentation is provided for solving the problems that the content of effective components in common medicinal and edible extracts is low, and the human body utilization rate is not high. The lactobacillus rhamnosus LR.M8 can effectively improve the adjusting effect of the rhizoma smilacis glabrae medicine and food homologous plant extract on uric acid balance, can reduce damage of inflammation to cells, and promotes in-vivo purine metabolism to recover balance. The fermentation method provided by the invention is simple and remarkable in effect, and has a wide application prospect.

The application discloses a pentapeptide IK5 with uric acid reducing activity as well as a preparation method and application thereof, and belongs to the technical field of small molecular peptides. The amino acid sequence of the pentapeptide IK5 is ITGYK, and the pentapeptide IK5 can be prepared by a solid-phase synthesis method and an enzymolysis method. The pentapeptide IK5 is identified from a protease enzymolysis liquid of a fine weak red winged seaweed, has potential interaction with xanthine oxidase, and can reduce the uric acid content of zebrafish by 44.4% (to 5.93+ / -0.47 mu mol / g protein) at a concentration of 100 mu g / mL. Compared with ananeine (which can reduce the uric acid content of zebrafish by 25.7%), the pentapeptide IK5 has better uric acid reducing activity. Compared with allopurinol (which can reduce the uric acid content of zebrafish to 4.61+ / -1.11 mu mol / g protein), the uric acid reducing ability of the pentapeptide IK5 and the allopurinol has no significant difference. The pentapeptide IK5 can be used for preparing uric acid reducing drugs and relieving hyperuricemia.

The invention relates to a food uric acid reducing composition. The food uric acid reducing composition comprises main materials and auxiliary materials, the mass ratio of the main material to the auxiliary material is 5: 1; the main material is prepared from the following raw materials in parts by weight: 10 to 15 parts of frankincense powder, 10 to 15 parts of tuna peptide, 50 to 100 parts of freeze-dried mulberry powder, 50 to 100 parts of soy isoflavone, 30 to 50 parts of red yeast rice powder, 10 to 20 parts of turmeric powder, 40 to 80 parts of marine red algae powder and 100 to 200 parts of celery seed powder; the auxiliary materials comprise at least one of the following auxiliary materials: a first auxiliary material which is selected from at least one of chicory powder, poria cocos powder and B vitamins; the second auxiliary material is selected from at least one of radix puerariae powder and lophatherum gracile powder; and the third auxiliary material is selected from at least one of sour cherry powder and licorice powder. According to the food uric acid reducing composition, the uric acid reducing effect is improved through combination of specific raw materials, the taste and taste of the product can be improved through food-borne materials, side effects are reduced, and the food uric acid reducing composition is suitable for being taken for a long time.

The invention provides a kiwi flower extract solid beverage with a uric acid reducing effect as well as a preparation method and application thereof, and belongs to the technical field of functional foods. Fresh yellow heart kiwi fruit flowers are sequentially subjected to drying, smashing, microwave enzyme deactivation and vacuum freeze drying operation to obtain kiwi fruit flower powder, then the kiwi fruit flower powder is subjected to gradient targeted extraction, firstly, subcritical water extraction is adopted, the flavone yield is increased, macromolecular impurities are removed through ceramic membrane filtration, an extracting solution A and filter residues are obtained, and the yellow heart kiwi fruit flower powder is obtained. The preparation method comprises the following steps: mixing filter residues with ethanol, then carrying out ultrasonic extraction to improve the extraction rate of polyphenol in the extract, carrying out suction filtration and concentration to obtain an extracting solution B, finally carrying out steady-state processing, mixing the extracting solution A and the extracting solution B, and carrying out freezing granulation to obtain the kiwi flower extract solid beverage with the effect of reducing uric acid and high content of flavonoid and high-phenol effective substances. The solid beverage of the kiwi flower extract has an excellent improvement effect on hyperuricemia.

The invention discloses a breeding method for intensively preventing and controlling goose gout and application of the breeding method, and aims to solve the technical problems of high incidence of goose gout and low survival rate in intensive goose breeding. According to the method, prevention and control are achieved through a cooperative scheme of graded disinfection, precise trace element supplement and environment regulation and control; 50-250 ppm hypochlorous acid is adopted for conducting graded sterilization on the goose house environment, a feeding trough and drinking water, and pathogen breeding and urate deposition inducements are cut off; when the geese are 15-75 days old, a German Q life water trace element solution is added into drinking water, the excretion function of the kidney is enhanced, and metabolic balance is adjusted; meanwhile, the breeding density is controlled to be 1-4 / m, the environment humidity is 55-70%, and ventilation and daily average activity amount are guaranteed. The method is accurate and safe in prevention and control and free of drug residues, the gout incidence rate of goose groups can be reduced to 4% or below, the survival rate is increased to 95% or above, the weight gain efficiency in the breeding period is increased by 10%-15%, the breeding cost and loss are remarkably reduced, the method is suitable for intensive breeding scenes of medium and small meat geese, breeding geese and the like, and comprehensive economic benefits are outstanding.

The invention relates to the field of bioelectrochemical sensors, in particular to an epitaxial gate type FET uric acid sensor and a uric acid concentration detection method. The FET uric acid sensor based on the epitaxial gate type comprises a field effect transistor and an epitaxial gate, the epitaxial grid electrode comprises ITO (Indium Tin Oxide) conductive glass connected with the grid electrode of the field effect transistor, an MXene / Ppy composite modification layer, a polypyrrole layer and a solution tank; the MXene / Ppy composite modification layer and the polypyrrole layer are sequentially deposited on the upper surface of the ITO conductive glass from bottom to top; the polypyrrole layer is provided with a specific imprinting cavity of uric acid to be detected; and the solution tank is arranged on the upper surface of the polypyrrole layer and is used for accommodating detection liquid. According to the invention, rapid, sensitive, accurate and low-cost detection of uric acid molecules in a low-concentration range can be realized.

The invention belongs to the technical field of medical detection, and particularly relates to a method and a system for detecting urate crystal deposition and phagocytosis in synovial organ tissues. The system comprises a synovial organ culture and verification module used for obtaining a human synovial sample, performing synovial tissue culture and forming different synovial organs; the stimulated Raman scattering microscope imaging module is used for imaging the cultured synovial organs; specifically, a specific pump-Stokes wavelength combination, a spatial deposition quantitative analysis and detection module of urate crystals and a spatial phagocytosis quantitative analysis and detection module of urate crystals are adopted; according to the method, the spatial deposition conditions of urate crystals in different synovials and the phagocytosis of osteoarthritis synovial cells on the crystals can be effectively imaged, and the unit quantity density and the intensity density of deposited or phagocytosed crystals are quantitatively calculated and analyzed; therefore, guidance is provided for development of related basic research and clinical quantitative diagnosis of gout.

This invention provides a traditional Chinese medicine composition for treating renal edema, pyelonephritis with ascites, and nephritis. It consists of over 20 herbs including Atractylodes lancea, Areca catechu, Saposhnikovia divaricata, and Alisma plantago-aquatica (specific formulation details can be found in the original text). It can be prepared as pills, granules, or decoctions for oral administration. Core efficacy: 1. Promotes diuresis and reduces swelling, eliminating renal ascites; 2. Clears heat and detoxifies, anti-inflammatory and antibacterial (synergistic effect on fungal infections and cervical erosion); 3. Regulates renal function and improves abnormal uric acid metabolism. Treatment plan: Mild cases: Use five basic formulas (①-⑤) composed of Codonopsis pilosula, Poria cocos, etc., taken three times daily; Severe cases: Use a potent diuretic formula containing Akebia trifoliata and Euphorbia pekinensis (one spoonful of pills twice daily); Treatment course is approximately one month, with a follow-up examination after three months. Advantages: Cure rate reaches 90%, low recurrence rate – pure traditional Chinese medicine preparation, no significant toxic side effects; also treats complications (fungal infection, cervical lesions). This treatment plan has been clinically verified through three generations and has significant efficacy in treating renal edema, ascites, and nephritis.

The present invention provides a method for quantitative detection of uric acid based on enzyme-free colorimetric sensing, belonging to the technical field of analysis and detection. The present invention obtains a blue product TMBox by mixing a buffer solution, a porous Co3O4 catalyst and 3,3',5,5'-tetramethylbenzidine and then filtering; then uric acid liquid is added to reduce TMBox, and after the reaction is completed, the uric acid concentration is judged according to the absorbance change of TMBox. The Co3O4 prepared by the present invention can catalyze the oxidation of TMB without hydrogen peroxide, and the method for quantitative detection of uric acid by enzyme-free colorimetric sensing established based on this does not require natural enzymes and artificial enzymes, and the uric acid detection range is wide (0-600 μmol / L) and the detection limit is low (0.33 μmol / L). The method for quantitative detection of uric acid by enzyme-free colorimetric sensing constructed by the present invention is applied to quantitative detection of uric acid, and the detection method is simple, convenient for promotion and application, and the detection time period is only 2-4min, which can be widely used in the determination of uric acid content in body fluids.

The invention belongs to the technical field of biological medicine, and particularly discloses a tea polyphenol self-polymerization nanoparticle for intracellular delivery of uricase and a preparation method and application thereof.The tea polyphenol self-polymerization nanoparticle comprises a tea polyphenol self-polymerization compound and uricase, the tea polyphenol self-polymerization compound is formed by preliminary self-polymerization of epigallocatechin gallate under catalysis of Mn < 2 + >, and the uricase is prepared from the tea polyphenol self-polymerization compound and the uricase; and mixing the polyphenol self-polymerized compound with uricase, and entrapping the uricase to obtain the tea polyphenol self-polymerized nanoparticles. The tea polyphenol self-polymerization nanoparticles have the functions of uricase combined delivery and synergistic treatment based on EGCG (epigallocatechin gallate) carriers (TPNs), the tertiary structure integrity of uricase can be effectively maintained, and the activity of uricase is ensured; meanwhile, uricase can be protected from being damaged by protease, and the stability of uricase is enhanced. Through the synergistic effect of uricase and TPNs in cells, both symptoms and root causes of intracellular urate crystallization and inflammatory reaction are treated, and a better scheme choice is provided for gout or hyperuricemia.

The invention relates to the technical field of protein drugs, in particular to a urate oxidase variant and a glycosylation modification form thereof. An engineered N-glycosylation site is introduced into urate oxidase, and a pair of engineered disulfide bonds is additionally introduced into a tetramer interface, so that the obtained glycosylation modified urate oxidase variant can improve folding stability, in-vivo performance and immunological properties on the basis of maintaining enzymatic activity.